Assessment of flavonoids contents and in vitro antioxidant activity of Launaea procumbens
<p>Abstract</p> <p>Background</p> <p><it>Launaea procumbens</it> (LP) has been used as a food supplement in Pakistan. In this study methanolic crude extract (LPME) of the whole plant and its different fractions; n-hexane (LPHE); ethyl acetate (LPEE) and chlo...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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BMC
2012-05-01
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| Series: | Chemistry Central Journal |
| Subjects: | |
| Online Access: | http://journal.chemistrycentral.com/content/6/1/43 |
| _version_ | 1818876616780021760 |
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| author | Khan Rahmat Khan Muhammad Sahreen Sumaira Ahmed Mushtaq |
| author_facet | Khan Rahmat Khan Muhammad Sahreen Sumaira Ahmed Mushtaq |
| author_sort | Khan Rahmat |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p><it>Launaea procumbens</it> (LP) has been used as a food supplement in Pakistan. In this study methanolic crude extract (LPME) of the whole plant and its different fractions; n-hexane (LPHE); ethyl acetate (LPEE) and chloroform (LPCE) were studied for the determination of total flavonoid and phenolics contents along with multifaceted <it>in vitro</it> scavenging assays.</p> <p>Results</p> <p>Considerable amount of flavonoid and phenolics contents were found in all the fractions. Methanol and chloroform fraction exhibited efficient scavenging of DPPH·, ABTS·+, ·OH, superoxide, lipid peroxide and nitric oxide free radicals. Significant correlation was found between DPPH·, ABTS·+, superoxide radical, β-carotene bleaching restraint and phosphomolybdenum assay with total flavonoids and phenolics contents. High performance chromatography (HPLC) of LPME revealed the presence of vitexin, orientin, rutin, hyperoside, catechin and myricetin.</p> <p>Conclusion</p> <p>These results reveal the presence of bioactive compounds in LPME, which might be contributed towards the various <it>in vitro</it> scavenging.</p> |
| first_indexed | 2024-12-19T13:45:14Z |
| format | Article |
| id | doaj.art-215c9e0280764c13a476fb1baed5ca2f |
| institution | Directory Open Access Journal |
| issn | 1752-153X |
| language | English |
| last_indexed | 2024-12-19T13:45:14Z |
| publishDate | 2012-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Chemistry Central Journal |
| spelling | doaj.art-215c9e0280764c13a476fb1baed5ca2f2022-12-21T20:18:52ZengBMCChemistry Central Journal1752-153X2012-05-01614310.1186/1752-153X-6-43Assessment of flavonoids contents and in vitro antioxidant activity of Launaea procumbensKhan RahmatKhan MuhammadSahreen SumairaAhmed Mushtaq<p>Abstract</p> <p>Background</p> <p><it>Launaea procumbens</it> (LP) has been used as a food supplement in Pakistan. In this study methanolic crude extract (LPME) of the whole plant and its different fractions; n-hexane (LPHE); ethyl acetate (LPEE) and chloroform (LPCE) were studied for the determination of total flavonoid and phenolics contents along with multifaceted <it>in vitro</it> scavenging assays.</p> <p>Results</p> <p>Considerable amount of flavonoid and phenolics contents were found in all the fractions. Methanol and chloroform fraction exhibited efficient scavenging of DPPH·, ABTS·+, ·OH, superoxide, lipid peroxide and nitric oxide free radicals. Significant correlation was found between DPPH·, ABTS·+, superoxide radical, β-carotene bleaching restraint and phosphomolybdenum assay with total flavonoids and phenolics contents. High performance chromatography (HPLC) of LPME revealed the presence of vitexin, orientin, rutin, hyperoside, catechin and myricetin.</p> <p>Conclusion</p> <p>These results reveal the presence of bioactive compounds in LPME, which might be contributed towards the various <it>in vitro</it> scavenging.</p>http://journal.chemistrycentral.com/content/6/1/43<it>Launaea procumbens</it>Scavenging of DPPH-free radicalsSuperoxide radicalsHPLCFlavonoids |
| spellingShingle | Khan Rahmat Khan Muhammad Sahreen Sumaira Ahmed Mushtaq Assessment of flavonoids contents and in vitro antioxidant activity of Launaea procumbens Chemistry Central Journal <it>Launaea procumbens</it> Scavenging of DPPH-free radicals Superoxide radicals HPLC Flavonoids |
| title | Assessment of flavonoids contents and in vitro antioxidant activity of Launaea procumbens |
| title_full | Assessment of flavonoids contents and in vitro antioxidant activity of Launaea procumbens |
| title_fullStr | Assessment of flavonoids contents and in vitro antioxidant activity of Launaea procumbens |
| title_full_unstemmed | Assessment of flavonoids contents and in vitro antioxidant activity of Launaea procumbens |
| title_short | Assessment of flavonoids contents and in vitro antioxidant activity of Launaea procumbens |
| title_sort | assessment of flavonoids contents and in vitro antioxidant activity of launaea procumbens |
| topic | <it>Launaea procumbens</it> Scavenging of DPPH-free radicals Superoxide radicals HPLC Flavonoids |
| url | http://journal.chemistrycentral.com/content/6/1/43 |
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