Molecular paleoparasitological diagnosis of Ascaris sp. from coprolites: new scenery of ascariasis in pre-Colombian South America times
Paleoparasitological studies using microscopy showed that Ascarisand Trichuris trichiura are the human intestinal parasites most found in archaeological sites. However, in pre-Columbian South American archaeological sites, Ascaris is rare. In this work we standardized a molecular methodology for Asc...
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2008-02-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762008000100017 |
| _version_ | 1797718202879311872 |
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| author | Daniela Leles Adauto Araújo Luiz Fernando Ferreira Ana Carolina Paulo Vicente Alena Mayo Iñiguez |
| author_facet | Daniela Leles Adauto Araújo Luiz Fernando Ferreira Ana Carolina Paulo Vicente Alena Mayo Iñiguez |
| author_sort | Daniela Leles |
| collection | DOAJ |
| description | Paleoparasitological studies using microscopy showed that Ascarisand Trichuris trichiura are the human intestinal parasites most found in archaeological sites. However, in pre-Columbian South American archaeological sites, Ascaris is rare. In this work we standardized a molecular methodology for Ascaris diagnosis directly from ancient DNA retrieved from coprolites. Using cythochrome b gene (142 bp) target, ancient DNA sequences were retrieved from South American samples, negative by microscopy. Moreover, the methodology applied was sensitive enough to detect ancient DNA extracted from 30 Ascaris eggs from an European coprolite. These results revealed a new scenery for the paleodistribution of Ascaris in South America. |
| first_indexed | 2024-03-12T08:47:30Z |
| format | Article |
| id | doaj.art-215ef9c1bdf24a5a87e6372b5dce2b78 |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T08:47:30Z |
| publishDate | 2008-02-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-215ef9c1bdf24a5a87e6372b5dce2b782023-09-02T16:25:08ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80602008-02-011031106108Molecular paleoparasitological diagnosis of Ascaris sp. from coprolites: new scenery of ascariasis in pre-Colombian South America timesDaniela LelesAdauto AraújoLuiz Fernando FerreiraAna Carolina Paulo VicenteAlena Mayo IñiguezPaleoparasitological studies using microscopy showed that Ascarisand Trichuris trichiura are the human intestinal parasites most found in archaeological sites. However, in pre-Columbian South American archaeological sites, Ascaris is rare. In this work we standardized a molecular methodology for Ascaris diagnosis directly from ancient DNA retrieved from coprolites. Using cythochrome b gene (142 bp) target, ancient DNA sequences were retrieved from South American samples, negative by microscopy. Moreover, the methodology applied was sensitive enough to detect ancient DNA extracted from 30 Ascaris eggs from an European coprolite. These results revealed a new scenery for the paleodistribution of Ascaris in South America.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762008000100017Ascarismolecular paleoparasitological diagnosispaleodistribution |
| spellingShingle | Daniela Leles Adauto Araújo Luiz Fernando Ferreira Ana Carolina Paulo Vicente Alena Mayo Iñiguez Molecular paleoparasitological diagnosis of Ascaris sp. from coprolites: new scenery of ascariasis in pre-Colombian South America times Memorias do Instituto Oswaldo Cruz Ascaris molecular paleoparasitological diagnosis paleodistribution |
| title | Molecular paleoparasitological diagnosis of Ascaris sp. from coprolites: new scenery of ascariasis in pre-Colombian South America times |
| title_full | Molecular paleoparasitological diagnosis of Ascaris sp. from coprolites: new scenery of ascariasis in pre-Colombian South America times |
| title_fullStr | Molecular paleoparasitological diagnosis of Ascaris sp. from coprolites: new scenery of ascariasis in pre-Colombian South America times |
| title_full_unstemmed | Molecular paleoparasitological diagnosis of Ascaris sp. from coprolites: new scenery of ascariasis in pre-Colombian South America times |
| title_short | Molecular paleoparasitological diagnosis of Ascaris sp. from coprolites: new scenery of ascariasis in pre-Colombian South America times |
| title_sort | molecular paleoparasitological diagnosis of ascaris sp from coprolites new scenery of ascariasis in pre colombian south america times |
| topic | Ascaris molecular paleoparasitological diagnosis paleodistribution |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762008000100017 |
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