Gut dysbiosis contributes to chlamydial induction of hydrosalpinx in the upper genital tract
Chlamydia trachomatis is one of the most common sexually infections that cause infertility, and its genital infection induces tubal adhesion and hydrosalpinx. Intravaginal Chlamydia muridarum infection in mice can induce hydrosalpinx in the upper genital tract and it has been used for studying C. tr...
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Frontiers Media S.A.
2023-04-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1142283/full |
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| author | Qi Tian Qi Tian Tianyuan Zhang Luying Wang Jingyue Ma Xin Sun |
| author_facet | Qi Tian Qi Tian Tianyuan Zhang Luying Wang Jingyue Ma Xin Sun |
| author_sort | Qi Tian |
| collection | DOAJ |
| description | Chlamydia trachomatis is one of the most common sexually infections that cause infertility, and its genital infection induces tubal adhesion and hydrosalpinx. Intravaginal Chlamydia muridarum infection in mice can induce hydrosalpinx in the upper genital tract and it has been used for studying C. trachomatis pathogenicity. DBA2/J strain mice were known to be resistant to the chlamydial induction of hydrosalpinx. In this study, we took advantage of this feature of DBA2/J mice to evaluate the role of antibiotic induced dysbiosis in chlamydial pathogenicity. Antibiotics (vancomycin and gentamicin) were orally administrated to induce dysbiosis in the gut of DBA2/J mice. The mice with or without antibiotic treatment were evaluated for gut and genital dysbiosis and then intravaginally challenged by C. muridarum. Chlamydial burden was tested and genital pathologies were evaluated. We found that oral antibiotics significantly enhanced chlamydial induction of genital hydrosalpinx. And the antibiotic treatment induced severe dysbiosis in the GI tract, including significantly reduced fecal DNA and increased ratios of firmicutes over bacteroidetes. The oral antibiotic did not alter chlamydial infection or microbiota in the mouse genital tracts. Our study showed that the oral antibiotics-enhanced hydrosalpinx correlated with dysbiosis in gut, providing the evidence for associating gut microbiome with chlamydial genital pathogenicity. |
| first_indexed | 2024-04-09T18:16:23Z |
| format | Article |
| id | doaj.art-21601764bf244be494fdca457bf477f5 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-09T18:16:23Z |
| publishDate | 2023-04-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-21601764bf244be494fdca457bf477f52023-04-13T04:25:05ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-04-011410.3389/fmicb.2023.11422831142283Gut dysbiosis contributes to chlamydial induction of hydrosalpinx in the upper genital tractQi Tian0Qi Tian1Tianyuan Zhang2Luying Wang3Jingyue Ma4Xin Sun5Department of Obstetrics and Gynecology, Hunan Provincial Maternal and Child Health Care Hospital, Changsha, Hunan, ChinaNational Health Commission Key Laboratory for Birth Defect Research and Prevention, Hunan Provincial Maternal and Child Health Care Hospital, Changsha, Hunan, ChinaKey Lab of Molecular Virology and Immunology, Institute Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, ChinaDepartment of Obstetrics and Gynecology, Third Xiangya Hospital, Central South University, Changsha, Hunan, ChinaDepartment of Dermatovenereology, Tianjin Medical University General Hospital, Tianjin, ChinaDepartment of Obstetrics and Gynecology, Third Xiangya Hospital, Central South University, Changsha, Hunan, ChinaChlamydia trachomatis is one of the most common sexually infections that cause infertility, and its genital infection induces tubal adhesion and hydrosalpinx. Intravaginal Chlamydia muridarum infection in mice can induce hydrosalpinx in the upper genital tract and it has been used for studying C. trachomatis pathogenicity. DBA2/J strain mice were known to be resistant to the chlamydial induction of hydrosalpinx. In this study, we took advantage of this feature of DBA2/J mice to evaluate the role of antibiotic induced dysbiosis in chlamydial pathogenicity. Antibiotics (vancomycin and gentamicin) were orally administrated to induce dysbiosis in the gut of DBA2/J mice. The mice with or without antibiotic treatment were evaluated for gut and genital dysbiosis and then intravaginally challenged by C. muridarum. Chlamydial burden was tested and genital pathologies were evaluated. We found that oral antibiotics significantly enhanced chlamydial induction of genital hydrosalpinx. And the antibiotic treatment induced severe dysbiosis in the GI tract, including significantly reduced fecal DNA and increased ratios of firmicutes over bacteroidetes. The oral antibiotic did not alter chlamydial infection or microbiota in the mouse genital tracts. Our study showed that the oral antibiotics-enhanced hydrosalpinx correlated with dysbiosis in gut, providing the evidence for associating gut microbiome with chlamydial genital pathogenicity.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1142283/fullOral antibioticsgut dysbiosisChlamydia muridarumgenital tractpathology |
| spellingShingle | Qi Tian Qi Tian Tianyuan Zhang Luying Wang Jingyue Ma Xin Sun Gut dysbiosis contributes to chlamydial induction of hydrosalpinx in the upper genital tract Frontiers in Microbiology Oral antibiotics gut dysbiosis Chlamydia muridarum genital tract pathology |
| title | Gut dysbiosis contributes to chlamydial induction of hydrosalpinx in the upper genital tract |
| title_full | Gut dysbiosis contributes to chlamydial induction of hydrosalpinx in the upper genital tract |
| title_fullStr | Gut dysbiosis contributes to chlamydial induction of hydrosalpinx in the upper genital tract |
| title_full_unstemmed | Gut dysbiosis contributes to chlamydial induction of hydrosalpinx in the upper genital tract |
| title_short | Gut dysbiosis contributes to chlamydial induction of hydrosalpinx in the upper genital tract |
| title_sort | gut dysbiosis contributes to chlamydial induction of hydrosalpinx in the upper genital tract |
| topic | Oral antibiotics gut dysbiosis Chlamydia muridarum genital tract pathology |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1142283/full |
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