Human mesenchymal stem cell secretome lowers caspase-3 levels and apoptosis in hepatocytes of cholestatic rats
Liver cirrhosis is ranked 15th as a cause of morbidity and is ranked 11th as a leading cause of mortality in the world. Definitive therapy is liver transplantation, but it is constrained by difficulties in finding a transplant donor Hu MSCs cell-based therapy can help liver regeneration directly, th...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Bangladesh Society for Microbiology, Immunology, and Advanced Biotechnology
2024-04-01
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| Series: | Journal of Advanced Biotechnology and Experimental Therapeutics |
| Subjects: | |
| Online Access: | https://www.bsmiab.org/jabet/?mno=163612 |
| _version_ | 1827355924223754240 |
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| author | Naufal Faruq Purwanto Neni Susilaningsih Bernardus Parish Budiono Nani Maharani Ignatius Riwanto |
| author_facet | Naufal Faruq Purwanto Neni Susilaningsih Bernardus Parish Budiono Nani Maharani Ignatius Riwanto |
| author_sort | Naufal Faruq Purwanto |
| collection | DOAJ |
| description | Liver cirrhosis is ranked 15th as a cause of morbidity and is ranked 11th as a leading cause of mortality in the world. Definitive therapy is liver transplantation, but it is constrained by difficulties in finding a transplant donor Hu MSCs cell-based therapy can help liver regeneration directly, through hepatogenic differentiation, or indirectly through the paracrine secretome. This study aims to determine the effect of Human Mesenchymal Stem Cell Secretome administration on caspase 3 levels and apoptotic hepatic cell count in a rat model with cholestasis after choledochal duct ligation receiving standard UDCA (Urso Deoxy Cholid Acid) therapy. Twenty-four male Wistar rats were subjected to a choledochal duct ligation and were included in this randomized experimental study. After surgical intervention, all rats were randomly assigned into 4 group; control, administration of UDCA, Hu MSC-S, and a combination of UDCA and Human Mesenchymal Stem Cell Secretome for 4 weeks. Caspase-3 levels were assessed from the blood sample, while the apoptosis of hepatocyte evaluated using histopathologic examination of the liver. Caspase-3 levels was significantly lower in the UDCA and Human Mesenchymal Stem Cell Secretome treated groups (6.23 ± 0,30), compared to the UDCA (8.77 ± 0.55) and Human Mesenchymal Stem Cell Secretome alone (7.30 ± 0.42) (ANOVA p < 0,05). The apoptosis of hepatocyte was significantly lower in the combination group (8,88 ± 5,46) compared to the UDCA (29,00 ± 4,52) and Human Mesenchymal Stem Cell Secretome alone (20,83 ± 4,14) (ANOVA p < 0,05). In conclusion, addition of Human Mesenchymal Stem Cell Secretome to UDCA lowered caspase-3 levels and apoptotic cell count in rats with hepatic cholestasis after choledochal duct ligation and better compared to each alone.
Keywords: Mesenchymal Stem Cell Secretome, Capsase-3, Apoptotic Cell, Liver Regeneration, Cholestasis [ J Adv Biotechnol Exp Ther 2024; 7(1.000): 44-52] |
| first_indexed | 2024-03-08T04:51:21Z |
| format | Article |
| id | doaj.art-2161452ac7e8481786885dc4a5ce672c |
| institution | Directory Open Access Journal |
| issn | 2616-4760 |
| language | English |
| last_indexed | 2024-03-08T04:51:21Z |
| publishDate | 2024-04-01 |
| publisher | Bangladesh Society for Microbiology, Immunology, and Advanced Biotechnology |
| record_format | Article |
| series | Journal of Advanced Biotechnology and Experimental Therapeutics |
| spelling | doaj.art-2161452ac7e8481786885dc4a5ce672c2024-02-08T05:03:01ZengBangladesh Society for Microbiology, Immunology, and Advanced BiotechnologyJournal of Advanced Biotechnology and Experimental Therapeutics2616-47602024-04-0171445210.5455/jabet.2024.d04163612Human mesenchymal stem cell secretome lowers caspase-3 levels and apoptosis in hepatocytes of cholestatic ratsNaufal Faruq Purwanto0Neni Susilaningsih1Bernardus Parish Budiono2Nani Maharani3Ignatius Riwanto4Faculty of Medicine, Diponegoro University, Semarang, Indonesia Department of Anatomy Histology, Faculty of Medicine, Diponegoro University, Semarang, Indonesia Department of Digestive Surgery, Dr Kariadi General Hospital / Faculty of Medicine, Diponegoro University, Semarang, Indonesia Department of Pharmacology and Therapeutic, Faculty of Medicine, Diponegoro University, Semarang, Indonesia Department of Digestive Surgery, Dr Kariadi General Hospital / Faculty of Medicine, Diponegoro University, Semarang, IndonesiaLiver cirrhosis is ranked 15th as a cause of morbidity and is ranked 11th as a leading cause of mortality in the world. Definitive therapy is liver transplantation, but it is constrained by difficulties in finding a transplant donor Hu MSCs cell-based therapy can help liver regeneration directly, through hepatogenic differentiation, or indirectly through the paracrine secretome. This study aims to determine the effect of Human Mesenchymal Stem Cell Secretome administration on caspase 3 levels and apoptotic hepatic cell count in a rat model with cholestasis after choledochal duct ligation receiving standard UDCA (Urso Deoxy Cholid Acid) therapy. Twenty-four male Wistar rats were subjected to a choledochal duct ligation and were included in this randomized experimental study. After surgical intervention, all rats were randomly assigned into 4 group; control, administration of UDCA, Hu MSC-S, and a combination of UDCA and Human Mesenchymal Stem Cell Secretome for 4 weeks. Caspase-3 levels were assessed from the blood sample, while the apoptosis of hepatocyte evaluated using histopathologic examination of the liver. Caspase-3 levels was significantly lower in the UDCA and Human Mesenchymal Stem Cell Secretome treated groups (6.23 ± 0,30), compared to the UDCA (8.77 ± 0.55) and Human Mesenchymal Stem Cell Secretome alone (7.30 ± 0.42) (ANOVA p < 0,05). The apoptosis of hepatocyte was significantly lower in the combination group (8,88 ± 5,46) compared to the UDCA (29,00 ± 4,52) and Human Mesenchymal Stem Cell Secretome alone (20,83 ± 4,14) (ANOVA p < 0,05). In conclusion, addition of Human Mesenchymal Stem Cell Secretome to UDCA lowered caspase-3 levels and apoptotic cell count in rats with hepatic cholestasis after choledochal duct ligation and better compared to each alone. Keywords: Mesenchymal Stem Cell Secretome, Capsase-3, Apoptotic Cell, Liver Regeneration, Cholestasis [ J Adv Biotechnol Exp Ther 2024; 7(1.000): 44-52]https://www.bsmiab.org/jabet/?mno=163612mesenchymal stem cell secretomecapsase-3apoptotic cellliver regenerationcholestasis |
| spellingShingle | Naufal Faruq Purwanto Neni Susilaningsih Bernardus Parish Budiono Nani Maharani Ignatius Riwanto Human mesenchymal stem cell secretome lowers caspase-3 levels and apoptosis in hepatocytes of cholestatic rats Journal of Advanced Biotechnology and Experimental Therapeutics mesenchymal stem cell secretome capsase-3 apoptotic cell liver regeneration cholestasis |
| title | Human mesenchymal stem cell secretome lowers caspase-3 levels and apoptosis in hepatocytes of cholestatic rats |
| title_full | Human mesenchymal stem cell secretome lowers caspase-3 levels and apoptosis in hepatocytes of cholestatic rats |
| title_fullStr | Human mesenchymal stem cell secretome lowers caspase-3 levels and apoptosis in hepatocytes of cholestatic rats |
| title_full_unstemmed | Human mesenchymal stem cell secretome lowers caspase-3 levels and apoptosis in hepatocytes of cholestatic rats |
| title_short | Human mesenchymal stem cell secretome lowers caspase-3 levels and apoptosis in hepatocytes of cholestatic rats |
| title_sort | human mesenchymal stem cell secretome lowers caspase 3 levels and apoptosis in hepatocytes of cholestatic rats |
| topic | mesenchymal stem cell secretome capsase-3 apoptotic cell liver regeneration cholestasis |
| url | https://www.bsmiab.org/jabet/?mno=163612 |
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