Prevalence of Genomic Resistance to Macrolide in Mycoplasma Isolates among Children with Community Acquired Pneumonia
Introduction: Mycoplasma pneumonia is traditionally susceptible to macrolides, tetracycline and fluroquinolones. Since, tetracycline and fluoroquinolone are used cautiously in children, macrolides remain the antibiotic of choice for treating Mycoplasma pneumonia. But, resistance to macrolides ha...
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| Format: | Article |
| Language: | English |
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JCDR Research and Publications Private Limited
2022-10-01
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| Series: | Journal of Clinical and Diagnostic Research |
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| Online Access: | https://www.jcdr.net/articles/PDF/17076/58540_CE(AD)_F(SK)_PF1(SC_SS)_PFA(SS)_PN(SS).pdf |
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| author | Sivasambo Kalpana Shanmugam Selvakumar Velmurugan Lakshmi Prabhu Dhandapani Prem Suruliraj |
| author_facet | Sivasambo Kalpana Shanmugam Selvakumar Velmurugan Lakshmi Prabhu Dhandapani Prem Suruliraj |
| author_sort | Sivasambo Kalpana |
| collection | DOAJ |
| description | Introduction: Mycoplasma pneumonia is traditionally
susceptible to macrolides, tetracycline and fluroquinolones.
Since, tetracycline and fluoroquinolone are used cautiously in
children, macrolides remain the antibiotic of choice for treating
Mycoplasma pneumonia. But, resistance to macrolides has
been reported in mycoplasma since the 2000s especially from
Asia. Currently, there is no evidence on macrolide resistance of
mycoplasma pneumoniae from India.
Aim: To identify the prevalence of genomic resistance to
macrolides in mycoplasma isolates among children hospitalised
with community acquired pneumonia.
Materials and Methods: This descriptive cross-sectional study
was conducted in Department of Paediatrics at Institute of
Child Health and Hospital for Children, Chennai, Tamil Nadu,
India (tertiary care centre for children in South India), from
September 2019 to August 2020. Children between 2 months
to 12 years of age, who were hospitalised with community
acquired pneumonia were included in the study. The sampling procedure used was induced sputum or mini bronchoalveolar
lavage (in intubated children). The samples were processed for
culture in Pleuropneumonia Like Organisms (PPLO) agar. The
culture isolates showing the typical fried egg colonies were
subjected to polymerase chain reaction to detect the presence of
resistance conferring mutation in the P1 adhesin gene MPN141.
Chi-square test was used to test statistical significance.
Results: Among the 268 children included in the study,
mycoplasma pneumonia was positive in 33 (12.3%) cases.
Mycoplasma pneumoniae was most common in children
aged 1-5 years old (51.5%), followed by infants (36.4%) and
children aged 5-12 (12.1%). There was no significant difference
in distribution among males (39.4%) and females (60.6%)
(p-value=0.08). None of the mycoplasma isolates in the study
showed mutation for resistance conferring genes.
Conclusion: Macrolide resistance conferring genes were not
identified in the study population, which may indicate that the
mycoplasma strains from this part of India are still susceptible
to macrolides. |
| first_indexed | 2024-04-10T22:16:02Z |
| format | Article |
| id | doaj.art-21627ba4aa83441fb37feb88cd1ca5f4 |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-04-10T22:16:02Z |
| publishDate | 2022-10-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-21627ba4aa83441fb37feb88cd1ca5f42023-01-18T08:27:23ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2022-10-011610SC15SC1710.7860/JCDR/2022/58540.17076Prevalence of Genomic Resistance to Macrolide in Mycoplasma Isolates among Children with Community Acquired PneumoniaSivasambo Kalpana0Shanmugam Selvakumar1 Velmurugan Lakshmi 2Prabhu Dhandapani3Prem Suruliraj4Assistant Professor, Department of Paediatrics, Institute of Child Health and Hospital for Children, Chennai, Tamil Nadu, India.Assistant Professor, Department of Paediatrics, Institute of Child Health and Hospital for Children, Chennai, Tamil Nadu, India.Professor, Department of Paediatrics, Institute of Child Health and Hospital for Children, Chennai, Tamil Nadu, India.Assistant Professor, Department of Microbiology, Dr. A.L.M. PG Institute of Basic Medical Sciences, Chennai, Tamil Nadu, India.Junior Resident, Department of Paediatrics, Institute of Child Health and Hospital for Children, Chennai, Tamil Nadu, India.Introduction: Mycoplasma pneumonia is traditionally susceptible to macrolides, tetracycline and fluroquinolones. Since, tetracycline and fluoroquinolone are used cautiously in children, macrolides remain the antibiotic of choice for treating Mycoplasma pneumonia. But, resistance to macrolides has been reported in mycoplasma since the 2000s especially from Asia. Currently, there is no evidence on macrolide resistance of mycoplasma pneumoniae from India. Aim: To identify the prevalence of genomic resistance to macrolides in mycoplasma isolates among children hospitalised with community acquired pneumonia. Materials and Methods: This descriptive cross-sectional study was conducted in Department of Paediatrics at Institute of Child Health and Hospital for Children, Chennai, Tamil Nadu, India (tertiary care centre for children in South India), from September 2019 to August 2020. Children between 2 months to 12 years of age, who were hospitalised with community acquired pneumonia were included in the study. The sampling procedure used was induced sputum or mini bronchoalveolar lavage (in intubated children). The samples were processed for culture in Pleuropneumonia Like Organisms (PPLO) agar. The culture isolates showing the typical fried egg colonies were subjected to polymerase chain reaction to detect the presence of resistance conferring mutation in the P1 adhesin gene MPN141. Chi-square test was used to test statistical significance. Results: Among the 268 children included in the study, mycoplasma pneumonia was positive in 33 (12.3%) cases. Mycoplasma pneumoniae was most common in children aged 1-5 years old (51.5%), followed by infants (36.4%) and children aged 5-12 (12.1%). There was no significant difference in distribution among males (39.4%) and females (60.6%) (p-value=0.08). None of the mycoplasma isolates in the study showed mutation for resistance conferring genes. Conclusion: Macrolide resistance conferring genes were not identified in the study population, which may indicate that the mycoplasma strains from this part of India are still susceptible to macrolides.https://www.jcdr.net/articles/PDF/17076/58540_CE(AD)_F(SK)_PF1(SC_SS)_PFA(SS)_PN(SS).pdfantibioticbronchoalveolar lavagemycoplasma pneumoniaemutationsusceptible |
| spellingShingle | Sivasambo Kalpana Shanmugam Selvakumar Velmurugan Lakshmi Prabhu Dhandapani Prem Suruliraj Prevalence of Genomic Resistance to Macrolide in Mycoplasma Isolates among Children with Community Acquired Pneumonia Journal of Clinical and Diagnostic Research antibiotic bronchoalveolar lavage mycoplasma pneumoniae mutation susceptible |
| title | Prevalence of Genomic Resistance to Macrolide in Mycoplasma Isolates among Children with Community Acquired Pneumonia |
| title_full | Prevalence of Genomic Resistance to Macrolide in Mycoplasma Isolates among Children with Community Acquired Pneumonia |
| title_fullStr | Prevalence of Genomic Resistance to Macrolide in Mycoplasma Isolates among Children with Community Acquired Pneumonia |
| title_full_unstemmed | Prevalence of Genomic Resistance to Macrolide in Mycoplasma Isolates among Children with Community Acquired Pneumonia |
| title_short | Prevalence of Genomic Resistance to Macrolide in Mycoplasma Isolates among Children with Community Acquired Pneumonia |
| title_sort | prevalence of genomic resistance to macrolide in mycoplasma isolates among children with community acquired pneumonia |
| topic | antibiotic bronchoalveolar lavage mycoplasma pneumoniae mutation susceptible |
| url | https://www.jcdr.net/articles/PDF/17076/58540_CE(AD)_F(SK)_PF1(SC_SS)_PFA(SS)_PN(SS).pdf |
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