Magnetic Resonance Imaging for Characterization of a Chick Embryo Model of Cancer Cell Metastases
Metastasis is the most common cause of death for patients with cancer. To fully understand the steps involved in metastatic dissemination, in vivo models are required, of which murine ones are the most common. Therefore, preclinical imaging methods such as magnetic resonance imaging (MRI) have mainl...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publications
2018-10-01
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| Series: | Molecular Imaging |
| Online Access: | https://doi.org/10.1177/1536012118809585 |
| _version_ | 1797283642424164352 |
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| author | Anne Herrmann PhD Arthur Taylor PhD Patricia Murray PhD Harish Poptani PhD Violaine Sée PhD |
| author_facet | Anne Herrmann PhD Arthur Taylor PhD Patricia Murray PhD Harish Poptani PhD Violaine Sée PhD |
| author_sort | Anne Herrmann PhD |
| collection | DOAJ |
| description | Metastasis is the most common cause of death for patients with cancer. To fully understand the steps involved in metastatic dissemination, in vivo models are required, of which murine ones are the most common. Therefore, preclinical imaging methods such as magnetic resonance imaging (MRI) have mainly been developed for small mammals and their potential to monitor cancer growth and metastasis in nonmammalian models is not fully harnessed. We have here used MRI to measure primary neuroblastoma tumor size and metastasis in a chick embryo model. We compared its sensitivity and accuracy to end-point fluorescence detection upon dissection. Human neuroblastoma cells labeled with green fluorescent protein (GFP) and micron-sized iron particles were implanted on the extraembryonic chorioallantoic membrane of the chick at E7. T 2 RARE, T 2 -weighted fast low angle shot (FLASH) as well as time-of-flight MR angiography imaging were applied at E14. Micron-sized iron particle labeling of neuroblastoma cells allowed in ovo observation of the primary tumor and tumor volume measurement noninvasively. Moreover, T 2 weighted and FLASH imaging permitted the detection of small metastatic deposits in the chick embryo, thereby reinforcing the potential of this convenient, 3R compliant, in vivo model for cancer research. |
| first_indexed | 2024-03-07T17:33:47Z |
| format | Article |
| id | doaj.art-216426f32cd94b24ad149328d7c9fa47 |
| institution | Directory Open Access Journal |
| issn | 1536-0121 |
| language | English |
| last_indexed | 2024-03-07T17:33:47Z |
| publishDate | 2018-10-01 |
| publisher | SAGE Publications |
| record_format | Article |
| series | Molecular Imaging |
| spelling | doaj.art-216426f32cd94b24ad149328d7c9fa472024-03-02T17:13:16ZengSAGE PublicationsMolecular Imaging1536-01212018-10-011710.1177/1536012118809585Magnetic Resonance Imaging for Characterization of a Chick Embryo Model of Cancer Cell MetastasesAnne Herrmann PhD0Arthur Taylor PhD1Patricia Murray PhD2Harish Poptani PhD3Violaine Sée PhD4 Department of Biochemistry, University of Liverpool, Liverpool, United Kingdom Centre for Preclinical Imaging, Department of Cellular and Molecular Physiology, University of Liverpool, Liverpool, United Kingdom Centre for Preclinical Imaging, Department of Cellular and Molecular Physiology, University of Liverpool, Liverpool, United Kingdom Centre for Preclinical Imaging, Department of Cellular and Molecular Physiology, University of Liverpool, Liverpool, United Kingdom Department of Biochemistry, University of Liverpool, Liverpool, United KingdomMetastasis is the most common cause of death for patients with cancer. To fully understand the steps involved in metastatic dissemination, in vivo models are required, of which murine ones are the most common. Therefore, preclinical imaging methods such as magnetic resonance imaging (MRI) have mainly been developed for small mammals and their potential to monitor cancer growth and metastasis in nonmammalian models is not fully harnessed. We have here used MRI to measure primary neuroblastoma tumor size and metastasis in a chick embryo model. We compared its sensitivity and accuracy to end-point fluorescence detection upon dissection. Human neuroblastoma cells labeled with green fluorescent protein (GFP) and micron-sized iron particles were implanted on the extraembryonic chorioallantoic membrane of the chick at E7. T 2 RARE, T 2 -weighted fast low angle shot (FLASH) as well as time-of-flight MR angiography imaging were applied at E14. Micron-sized iron particle labeling of neuroblastoma cells allowed in ovo observation of the primary tumor and tumor volume measurement noninvasively. Moreover, T 2 weighted and FLASH imaging permitted the detection of small metastatic deposits in the chick embryo, thereby reinforcing the potential of this convenient, 3R compliant, in vivo model for cancer research.https://doi.org/10.1177/1536012118809585 |
| spellingShingle | Anne Herrmann PhD Arthur Taylor PhD Patricia Murray PhD Harish Poptani PhD Violaine Sée PhD Magnetic Resonance Imaging for Characterization of a Chick Embryo Model of Cancer Cell Metastases Molecular Imaging |
| title | Magnetic Resonance Imaging for Characterization of a Chick Embryo Model of Cancer Cell Metastases |
| title_full | Magnetic Resonance Imaging for Characterization of a Chick Embryo Model of Cancer Cell Metastases |
| title_fullStr | Magnetic Resonance Imaging for Characterization of a Chick Embryo Model of Cancer Cell Metastases |
| title_full_unstemmed | Magnetic Resonance Imaging for Characterization of a Chick Embryo Model of Cancer Cell Metastases |
| title_short | Magnetic Resonance Imaging for Characterization of a Chick Embryo Model of Cancer Cell Metastases |
| title_sort | magnetic resonance imaging for characterization of a chick embryo model of cancer cell metastases |
| url | https://doi.org/10.1177/1536012118809585 |
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