Restoring Genetic Resource through In Vitro Culturing Testicular Cells from the Cryo-Preserved Tissue of the American Shad (<i>Alosa sapidissima</i>)
Germ cells, as opposed to somatic cells, can transmit heredity information between generations. Cryopreservation and in vitro culture of germ cells are key techniques for genetic resource preservation and cellular engineering breeding. In this study, two types of cryopreserved samples, namely testis...
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MDPI AG
2022-05-01
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| author | Hong-Yan Xu Xiao-You Hong Chao-Yue Zhong Xu-Ling Wu Xin-Ping Zhu |
| author_facet | Hong-Yan Xu Xiao-You Hong Chao-Yue Zhong Xu-Ling Wu Xin-Ping Zhu |
| author_sort | Hong-Yan Xu |
| collection | DOAJ |
| description | Germ cells, as opposed to somatic cells, can transmit heredity information between generations. Cryopreservation and in vitro culture of germ cells are key techniques for genetic resource preservation and cellular engineering breeding. In this study, two types of cryopreserved samples, namely testis pieces and testicular cells of American shad, were comparatively analyzed for cell viability. The results showed that the cell viability of the cryopreserved testis pieces was much higher than that of the cryopreserved testicular cells. The viability of cells from the cryopreserved testis pieces ranged from 65.2 ± 2.2 (%) to 93.8 ± 0.6 (%), whereas the viability of the dissociated cells after cryopreservation was 38.5 ± 0.8 (%) to 87.1 ± 2.6 (%). Intriguingly, the testicular cells from the post-thaw testicular tissue could be cultured in vitro. Likewise, most of the cultured cells exhibited germ cell properties and highly expressed Vasa and PCNA protein. This study is the first attempt to effectively preserve and culture the male germ cells through freezing tissues in the American shad. The findings of this study would benefit further investigations on genetic resource preservation and other manipulations of germ cells in a commercially and ecologically important fish species. |
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| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-10T03:18:09Z |
| publishDate | 2022-05-01 |
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| spelling | doaj.art-21694faabd234c1abe0a9ab66584fc602023-11-23T10:08:29ZengMDPI AGBiology2079-77372022-05-0111579010.3390/biology11050790Restoring Genetic Resource through In Vitro Culturing Testicular Cells from the Cryo-Preserved Tissue of the American Shad (<i>Alosa sapidissima</i>)Hong-Yan Xu0Xiao-You Hong1Chao-Yue Zhong2Xu-Ling Wu3Xin-Ping Zhu4Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Sciences of Chongqing, College of Fisheries, Southwest University, Chongqing 402460, ChinaKey Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation of Ministry of Agriculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, ChinaKey Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation of Ministry of Agriculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, ChinaKey Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation of Ministry of Agriculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, ChinaKey Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation of Ministry of Agriculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, ChinaGerm cells, as opposed to somatic cells, can transmit heredity information between generations. Cryopreservation and in vitro culture of germ cells are key techniques for genetic resource preservation and cellular engineering breeding. In this study, two types of cryopreserved samples, namely testis pieces and testicular cells of American shad, were comparatively analyzed for cell viability. The results showed that the cell viability of the cryopreserved testis pieces was much higher than that of the cryopreserved testicular cells. The viability of cells from the cryopreserved testis pieces ranged from 65.2 ± 2.2 (%) to 93.8 ± 0.6 (%), whereas the viability of the dissociated cells after cryopreservation was 38.5 ± 0.8 (%) to 87.1 ± 2.6 (%). Intriguingly, the testicular cells from the post-thaw testicular tissue could be cultured in vitro. Likewise, most of the cultured cells exhibited germ cell properties and highly expressed Vasa and PCNA protein. This study is the first attempt to effectively preserve and culture the male germ cells through freezing tissues in the American shad. The findings of this study would benefit further investigations on genetic resource preservation and other manipulations of germ cells in a commercially and ecologically important fish species.https://www.mdpi.com/2079-7737/11/5/790male germ cellscryopreservation of genetic resourcecell cultureAmerican shad |
| spellingShingle | Hong-Yan Xu Xiao-You Hong Chao-Yue Zhong Xu-Ling Wu Xin-Ping Zhu Restoring Genetic Resource through In Vitro Culturing Testicular Cells from the Cryo-Preserved Tissue of the American Shad (<i>Alosa sapidissima</i>) Biology male germ cells cryopreservation of genetic resource cell culture American shad |
| title | Restoring Genetic Resource through In Vitro Culturing Testicular Cells from the Cryo-Preserved Tissue of the American Shad (<i>Alosa sapidissima</i>) |
| title_full | Restoring Genetic Resource through In Vitro Culturing Testicular Cells from the Cryo-Preserved Tissue of the American Shad (<i>Alosa sapidissima</i>) |
| title_fullStr | Restoring Genetic Resource through In Vitro Culturing Testicular Cells from the Cryo-Preserved Tissue of the American Shad (<i>Alosa sapidissima</i>) |
| title_full_unstemmed | Restoring Genetic Resource through In Vitro Culturing Testicular Cells from the Cryo-Preserved Tissue of the American Shad (<i>Alosa sapidissima</i>) |
| title_short | Restoring Genetic Resource through In Vitro Culturing Testicular Cells from the Cryo-Preserved Tissue of the American Shad (<i>Alosa sapidissima</i>) |
| title_sort | restoring genetic resource through in vitro culturing testicular cells from the cryo preserved tissue of the american shad i alosa sapidissima i |
| topic | male germ cells cryopreservation of genetic resource cell culture American shad |
| url | https://www.mdpi.com/2079-7737/11/5/790 |
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