The E3 Ubiquitin Ligase TRIM25 Inhibits Tembusu Virus Replication in vitro
Duck Tembusu virus (DTMUV) is a newly emerging pathogenic flavivirus that has caused significant economic losses to the duck industry in China since 2010 due to egg production losses and neurological dysfunction. DTMUV is a public health concern because the infection spreads rapidly among birds. Ret...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-09-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2021.722113/full |
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| author | Han Kaikai Han Kaikai Han Kaikai Han Kaikai Dongmin Zhao Dongmin Zhao Dongmin Zhao Yuzhuo Liu Qingtao Liu Qingtao Liu Qingtao Liu Xinmei Huang Xinmei Huang Xinmei Huang Jing Yang Lijiao Zhang Yin Li Yin Li Yin Li |
| author_facet | Han Kaikai Han Kaikai Han Kaikai Han Kaikai Dongmin Zhao Dongmin Zhao Dongmin Zhao Yuzhuo Liu Qingtao Liu Qingtao Liu Qingtao Liu Xinmei Huang Xinmei Huang Xinmei Huang Jing Yang Lijiao Zhang Yin Li Yin Li Yin Li |
| author_sort | Han Kaikai |
| collection | DOAJ |
| description | Duck Tembusu virus (DTMUV) is a newly emerging pathogenic flavivirus that has caused significant economic losses to the duck industry in China since 2010 due to egg production losses and neurological dysfunction. DTMUV is a public health concern because the infection spreads rapidly among birds. Retinoic acid-inducible gene-I (RIG-I)serves as an innate immune sensor and plays a key role in host antiviral defenses. Tripartite motif-containing protein 25 (TRIM25), an E3 ubiquitin ligase, is pivotal for RIG-I ubiquitination and activation. In addition, TRIM25 acts as an interferon-stimulated gene and mediates the antiviral activity. However, the effect of duck TRIM25 on DTMUV has not been assessed. Herein, we reportthe antiviral function of TRIM25 against DTMUV. First, we constructed the pcDNA3.1-c-myc-duTRIM25 plasmid. TRIM25 has a 2052 bp open reading frame that encodes a predicted 684 amino acid protein consisting of a RING finger domain, a B-box domain, a coiled-coil domain, and a PRY/SPRY domain. The protein sequence identity with chicken, mouse, and human TRIM25 is 69.7, 47.8, and 48.3%, respectively. TRIM25 was upregulated in BHK-21 cells, duck embryo fibroblasts, and 293T cellsupon DTMUV infection. The expression of viral RNA and proteins was significantly lower in cells over expressing TRIM25 than in control cells. Furthermore, siRNA-mediated silencing of TRIM25 increased the production of viral progeny. These results help elucidate the molecular mechanisms underlying the host response to DTMUV infection and suggest potential control measures for DTMUV outbreaks. |
| first_indexed | 2024-12-21T20:23:53Z |
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| id | doaj.art-216a8c6c09644ebba56a4d180b9a15b2 |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-12-21T20:23:53Z |
| publishDate | 2021-09-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Veterinary Science |
| spelling | doaj.art-216a8c6c09644ebba56a4d180b9a15b22022-12-21T18:51:26ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692021-09-01810.3389/fvets.2021.722113722113The E3 Ubiquitin Ligase TRIM25 Inhibits Tembusu Virus Replication in vitroHan Kaikai0Han Kaikai1Han Kaikai2Han Kaikai3Dongmin Zhao4Dongmin Zhao5Dongmin Zhao6Yuzhuo Liu7Qingtao Liu8Qingtao Liu9Qingtao Liu10Xinmei Huang11Xinmei Huang12Xinmei Huang13Jing Yang14Lijiao Zhang15Yin Li16Yin Li17Yin Li18Key Laboratory of Veterinary Diagnosis, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaInstitute of Life Sciences, Jiangsu University, Zhenjiang, ChinaCollege of Veterinary Medicine, Nanjing Agricultural University, Nanjing, ChinaJiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology, Nanjing, ChinaKey Laboratory of Veterinary Diagnosis, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaInstitute of Life Sciences, Jiangsu University, Zhenjiang, ChinaCollege of Veterinary Medicine, Nanjing Agricultural University, Nanjing, ChinaKey Laboratory of Veterinary Diagnosis, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaKey Laboratory of Veterinary Diagnosis, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaInstitute of Life Sciences, Jiangsu University, Zhenjiang, ChinaCollege of Veterinary Medicine, Nanjing Agricultural University, Nanjing, ChinaKey Laboratory of Veterinary Diagnosis, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaInstitute of Life Sciences, Jiangsu University, Zhenjiang, ChinaCollege of Veterinary Medicine, Nanjing Agricultural University, Nanjing, ChinaKey Laboratory of Veterinary Diagnosis, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaKey Laboratory of Veterinary Diagnosis, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaKey Laboratory of Veterinary Diagnosis, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaInstitute of Life Sciences, Jiangsu University, Zhenjiang, ChinaCollege of Veterinary Medicine, Nanjing Agricultural University, Nanjing, ChinaDuck Tembusu virus (DTMUV) is a newly emerging pathogenic flavivirus that has caused significant economic losses to the duck industry in China since 2010 due to egg production losses and neurological dysfunction. DTMUV is a public health concern because the infection spreads rapidly among birds. Retinoic acid-inducible gene-I (RIG-I)serves as an innate immune sensor and plays a key role in host antiviral defenses. Tripartite motif-containing protein 25 (TRIM25), an E3 ubiquitin ligase, is pivotal for RIG-I ubiquitination and activation. In addition, TRIM25 acts as an interferon-stimulated gene and mediates the antiviral activity. However, the effect of duck TRIM25 on DTMUV has not been assessed. Herein, we reportthe antiviral function of TRIM25 against DTMUV. First, we constructed the pcDNA3.1-c-myc-duTRIM25 plasmid. TRIM25 has a 2052 bp open reading frame that encodes a predicted 684 amino acid protein consisting of a RING finger domain, a B-box domain, a coiled-coil domain, and a PRY/SPRY domain. The protein sequence identity with chicken, mouse, and human TRIM25 is 69.7, 47.8, and 48.3%, respectively. TRIM25 was upregulated in BHK-21 cells, duck embryo fibroblasts, and 293T cellsupon DTMUV infection. The expression of viral RNA and proteins was significantly lower in cells over expressing TRIM25 than in control cells. Furthermore, siRNA-mediated silencing of TRIM25 increased the production of viral progeny. These results help elucidate the molecular mechanisms underlying the host response to DTMUV infection and suggest potential control measures for DTMUV outbreaks.https://www.frontiersin.org/articles/10.3389/fvets.2021.722113/fullduckTembusu virusTRIM25tissue distributionviral replication |
| spellingShingle | Han Kaikai Han Kaikai Han Kaikai Han Kaikai Dongmin Zhao Dongmin Zhao Dongmin Zhao Yuzhuo Liu Qingtao Liu Qingtao Liu Qingtao Liu Xinmei Huang Xinmei Huang Xinmei Huang Jing Yang Lijiao Zhang Yin Li Yin Li Yin Li The E3 Ubiquitin Ligase TRIM25 Inhibits Tembusu Virus Replication in vitro Frontiers in Veterinary Science duck Tembusu virus TRIM25 tissue distribution viral replication |
| title | The E3 Ubiquitin Ligase TRIM25 Inhibits Tembusu Virus Replication in vitro |
| title_full | The E3 Ubiquitin Ligase TRIM25 Inhibits Tembusu Virus Replication in vitro |
| title_fullStr | The E3 Ubiquitin Ligase TRIM25 Inhibits Tembusu Virus Replication in vitro |
| title_full_unstemmed | The E3 Ubiquitin Ligase TRIM25 Inhibits Tembusu Virus Replication in vitro |
| title_short | The E3 Ubiquitin Ligase TRIM25 Inhibits Tembusu Virus Replication in vitro |
| title_sort | e3 ubiquitin ligase trim25 inhibits tembusu virus replication in vitro |
| topic | duck Tembusu virus TRIM25 tissue distribution viral replication |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2021.722113/full |
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