Monkey Viperin Restricts Porcine Reproductive and Respiratory Syndrome Virus Replication.
Porcine reproductive and respiratory syndrome virus (PRRSV) is an important pathogen which causes huge economic damage globally in the swine industry. Current vaccination strategies provide only limited protection against PRRSV infection. Viperin is an interferon (IFN) stimulated protein that inhibi...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2016-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4883763?pdf=render |
| _version_ | 1818304817681924096 |
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| author | Jianyu Fang Haiyan Wang Juan Bai Qiaoya Zhang Yufeng Li Fei Liu Ping Jiang |
| author_facet | Jianyu Fang Haiyan Wang Juan Bai Qiaoya Zhang Yufeng Li Fei Liu Ping Jiang |
| author_sort | Jianyu Fang |
| collection | DOAJ |
| description | Porcine reproductive and respiratory syndrome virus (PRRSV) is an important pathogen which causes huge economic damage globally in the swine industry. Current vaccination strategies provide only limited protection against PRRSV infection. Viperin is an interferon (IFN) stimulated protein that inhibits some virus infections via IFN-dependent or IFN-independent pathways. However, the role of viperin in PRRSV infection is not well understood. In this study, we cloned the full-length monkey viperin (mViperin) complementary DNA (cDNA) from IFN-α-treated African green monkey Marc-145 cells. It was found that the mViperin is up-regulated following PRRSV infection in Marc-145 cells along with elevated IRF-1 gene levels. IFN-α induced mViperin expression in a dose- and time-dependent manner and strongly inhibits PRRSV replication in Marc-145 cells. Overexpression of mViperin suppresses PRRSV replication by blocking the early steps of PRRSV entry and genome replication and translation but not inhibiting assembly and release. And mViperin co-localized with PRRSV GP5 and N protein, but only interacted with N protein in distinct cytoplasmic loci. Furthermore, it was found that the 13-16 amino acids of mViperin were essential for inhibiting PRRSV replication, by disrupting the distribution of mViperin protein from the granular distribution to a homogeneous distribution in the cytoplasm. These results could be helpful in the future development of novel antiviral therapies against PRRSV infection. |
| first_indexed | 2024-12-13T06:16:44Z |
| format | Article |
| id | doaj.art-216bffbdb944483b9a807df185361259 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T06:16:44Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS ONE |
| spelling | doaj.art-216bffbdb944483b9a807df1853612592022-12-21T23:56:56ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01115e015651310.1371/journal.pone.0156513Monkey Viperin Restricts Porcine Reproductive and Respiratory Syndrome Virus Replication.Jianyu FangHaiyan WangJuan BaiQiaoya ZhangYufeng LiFei LiuPing JiangPorcine reproductive and respiratory syndrome virus (PRRSV) is an important pathogen which causes huge economic damage globally in the swine industry. Current vaccination strategies provide only limited protection against PRRSV infection. Viperin is an interferon (IFN) stimulated protein that inhibits some virus infections via IFN-dependent or IFN-independent pathways. However, the role of viperin in PRRSV infection is not well understood. In this study, we cloned the full-length monkey viperin (mViperin) complementary DNA (cDNA) from IFN-α-treated African green monkey Marc-145 cells. It was found that the mViperin is up-regulated following PRRSV infection in Marc-145 cells along with elevated IRF-1 gene levels. IFN-α induced mViperin expression in a dose- and time-dependent manner and strongly inhibits PRRSV replication in Marc-145 cells. Overexpression of mViperin suppresses PRRSV replication by blocking the early steps of PRRSV entry and genome replication and translation but not inhibiting assembly and release. And mViperin co-localized with PRRSV GP5 and N protein, but only interacted with N protein in distinct cytoplasmic loci. Furthermore, it was found that the 13-16 amino acids of mViperin were essential for inhibiting PRRSV replication, by disrupting the distribution of mViperin protein from the granular distribution to a homogeneous distribution in the cytoplasm. These results could be helpful in the future development of novel antiviral therapies against PRRSV infection.http://europepmc.org/articles/PMC4883763?pdf=render |
| spellingShingle | Jianyu Fang Haiyan Wang Juan Bai Qiaoya Zhang Yufeng Li Fei Liu Ping Jiang Monkey Viperin Restricts Porcine Reproductive and Respiratory Syndrome Virus Replication. PLoS ONE |
| title | Monkey Viperin Restricts Porcine Reproductive and Respiratory Syndrome Virus Replication. |
| title_full | Monkey Viperin Restricts Porcine Reproductive and Respiratory Syndrome Virus Replication. |
| title_fullStr | Monkey Viperin Restricts Porcine Reproductive and Respiratory Syndrome Virus Replication. |
| title_full_unstemmed | Monkey Viperin Restricts Porcine Reproductive and Respiratory Syndrome Virus Replication. |
| title_short | Monkey Viperin Restricts Porcine Reproductive and Respiratory Syndrome Virus Replication. |
| title_sort | monkey viperin restricts porcine reproductive and respiratory syndrome virus replication |
| url | http://europepmc.org/articles/PMC4883763?pdf=render |
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