Polyvinylpyrrolidone—Alginate—Carbonate Hydroxyapatite Porous Composites for Dental Applications
An alternative approach for the currently used replacement therapy in dentistry is to apply materials that restore tooth tissue. Among them, composites, based on biopolymers with calcium phosphates, and cells can be applied. In the present work, a composite based on polyvinylpyrrolidone (PVP) and al...
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2023-06-01
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| author | Anna A. Forysenkova Inna V. Fadeeva Dina V. Deyneko Alevtina N. Gosteva Georgy V. Mamin Darya V. Shurtakova Galina A. Davydova Viktoriya G. Yankova Iulian V. Antoniac Julietta V. Rau |
| author_facet | Anna A. Forysenkova Inna V. Fadeeva Dina V. Deyneko Alevtina N. Gosteva Georgy V. Mamin Darya V. Shurtakova Galina A. Davydova Viktoriya G. Yankova Iulian V. Antoniac Julietta V. Rau |
| author_sort | Anna A. Forysenkova |
| collection | DOAJ |
| description | An alternative approach for the currently used replacement therapy in dentistry is to apply materials that restore tooth tissue. Among them, composites, based on biopolymers with calcium phosphates, and cells can be applied. In the present work, a composite based on polyvinylpyrrolidone (PVP) and alginate (Alg) with carbonate hydroxyapatite (CHA) was prepared and characterized. The composite was investigated by X-ray diffraction, infrared spectroscopy, electron paramagnetic resonance (EPR) and scanning electron microscopy methods, and the microstructure, porosity, and swelling properties of the material were described. In vitro studies included the MTT test using mouse fibroblasts, and adhesion and survivability tests with human dental pulp stem cells (DPSC). The mineral component of the composite corresponded to CHA with an admixture of amorphous calcium phosphate. The presence of a bond between the polymer matrix and CHA particles was shown by EPR. The structure of the material was represented by micro- (30–190 μm) and nano-pores (average 8.71 ± 4.15 nm). The swelling measurements attested that CHA addition increased the polymer matrix hydrophilicity by 200%. <i>In vitro</i> studies demonstrated the biocompatibility of PVP-Alg-CHA (95 ± 5% cell viability), and DPSC located inside the pores. It was concluded that the PVP-Alg-CHA porous composite is promising for dentistry applications. |
| first_indexed | 2024-03-11T02:12:08Z |
| format | Article |
| id | doaj.art-216e022ddf3f4a98a241e1bec66da9ae |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-11T02:12:08Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Materials |
| spelling | doaj.art-216e022ddf3f4a98a241e1bec66da9ae2023-11-18T11:26:50ZengMDPI AGMaterials1996-19442023-06-011612447810.3390/ma16124478Polyvinylpyrrolidone—Alginate—Carbonate Hydroxyapatite Porous Composites for Dental ApplicationsAnna A. Forysenkova0Inna V. Fadeeva1Dina V. Deyneko2Alevtina N. Gosteva3Georgy V. Mamin4Darya V. Shurtakova5Galina A. Davydova6Viktoriya G. Yankova7Iulian V. Antoniac8Julietta V. Rau9A.A. Baikov Institute of Metallurgy and Material Science RAS, Leninsky, 49, 119334 Moscow, RussiaA.A. Baikov Institute of Metallurgy and Material Science RAS, Leninsky, 49, 119334 Moscow, RussiaChemistry Department, Lomonosov Moscow State University, Leninskie Gory 1, 119991 Moscow, RussiaTananaev Institute of Chemistry, Kola Science Centre RAS, Akademgorodok 26A, 184209 Apatity, RussiaInstitute of Physics, Kazan Federal University, Kremlevskaya 18, 420008 Kazan, RussiaInstitute of Physics, Kazan Federal University, Kremlevskaya 18, 420008 Kazan, RussiaInstitute of Theoretical and Experimental Biophysics of RAS, Institutskaya 3, Puschino, 142290 Moscow, RussiaDepartment of Analytical, Physical and Colloid Chemistry, Institute of Pharmacy, I.M. Sechenov First Moscow State Medical University, Trubetskaya 8, Build. 2, 119991 Moscow, RussiaFaculty of Materials Science and Engineering, University Politehnica of Bucharest, 313 Splaiul Independentei Street, District 6, 060042 Bucharest, RomaniaDepartment of Analytical, Physical and Colloid Chemistry, Institute of Pharmacy, I.M. Sechenov First Moscow State Medical University, Trubetskaya 8, Build. 2, 119991 Moscow, RussiaAn alternative approach for the currently used replacement therapy in dentistry is to apply materials that restore tooth tissue. Among them, composites, based on biopolymers with calcium phosphates, and cells can be applied. In the present work, a composite based on polyvinylpyrrolidone (PVP) and alginate (Alg) with carbonate hydroxyapatite (CHA) was prepared and characterized. The composite was investigated by X-ray diffraction, infrared spectroscopy, electron paramagnetic resonance (EPR) and scanning electron microscopy methods, and the microstructure, porosity, and swelling properties of the material were described. In vitro studies included the MTT test using mouse fibroblasts, and adhesion and survivability tests with human dental pulp stem cells (DPSC). The mineral component of the composite corresponded to CHA with an admixture of amorphous calcium phosphate. The presence of a bond between the polymer matrix and CHA particles was shown by EPR. The structure of the material was represented by micro- (30–190 μm) and nano-pores (average 8.71 ± 4.15 nm). The swelling measurements attested that CHA addition increased the polymer matrix hydrophilicity by 200%. <i>In vitro</i> studies demonstrated the biocompatibility of PVP-Alg-CHA (95 ± 5% cell viability), and DPSC located inside the pores. It was concluded that the PVP-Alg-CHA porous composite is promising for dentistry applications.https://www.mdpi.com/1996-1944/16/12/4478compositehydroxyapatitecarbonate hydroxyapatitealginatepolyvinylpyrrolidone |
| spellingShingle | Anna A. Forysenkova Inna V. Fadeeva Dina V. Deyneko Alevtina N. Gosteva Georgy V. Mamin Darya V. Shurtakova Galina A. Davydova Viktoriya G. Yankova Iulian V. Antoniac Julietta V. Rau Polyvinylpyrrolidone—Alginate—Carbonate Hydroxyapatite Porous Composites for Dental Applications Materials composite hydroxyapatite carbonate hydroxyapatite alginate polyvinylpyrrolidone |
| title | Polyvinylpyrrolidone—Alginate—Carbonate Hydroxyapatite Porous Composites for Dental Applications |
| title_full | Polyvinylpyrrolidone—Alginate—Carbonate Hydroxyapatite Porous Composites for Dental Applications |
| title_fullStr | Polyvinylpyrrolidone—Alginate—Carbonate Hydroxyapatite Porous Composites for Dental Applications |
| title_full_unstemmed | Polyvinylpyrrolidone—Alginate—Carbonate Hydroxyapatite Porous Composites for Dental Applications |
| title_short | Polyvinylpyrrolidone—Alginate—Carbonate Hydroxyapatite Porous Composites for Dental Applications |
| title_sort | polyvinylpyrrolidone alginate carbonate hydroxyapatite porous composites for dental applications |
| topic | composite hydroxyapatite carbonate hydroxyapatite alginate polyvinylpyrrolidone |
| url | https://www.mdpi.com/1996-1944/16/12/4478 |
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