Rhamnolipid 89 Biosurfactant Is Effective against <i>Streptococcus oralis</i> Biofilm and Preserves Osteoblast Behavior: Perspectives in Dental Implantology
Biofilm-related peri-implant diseases represent the major complication for osteointegrated dental implants, requiring complex treatments or implant removal. Microbial biosurfactants emerged as new antibiofilm coating agents for implantable devices thanks to their high biocompatibility. This study ai...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-09-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/18/14014 |
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| author | Erica Tambone Chiara Ceresa Alice Marchetti Silvia Chiera Adriano Anesi Giandomenico Nollo Iole Caola Michela Bosetti Letizia Fracchia Paolo Ghensi Francesco Tessarolo |
| author_facet | Erica Tambone Chiara Ceresa Alice Marchetti Silvia Chiera Adriano Anesi Giandomenico Nollo Iole Caola Michela Bosetti Letizia Fracchia Paolo Ghensi Francesco Tessarolo |
| author_sort | Erica Tambone |
| collection | DOAJ |
| description | Biofilm-related peri-implant diseases represent the major complication for osteointegrated dental implants, requiring complex treatments or implant removal. Microbial biosurfactants emerged as new antibiofilm coating agents for implantable devices thanks to their high biocompatibility. This study aimed to assess the efficacy of the rhamnolipid 89 biosurfactant (R89BS) in limiting <i>Streptococcus oralis</i> biofilm formation and dislodging sessile cells from medical grade titanium, but preserving adhesion and proliferation of human osteoblasts. The inhibitory activity of a R89BS coating on <i>S. oralis</i> biofilm formation was assayed by quantifying biofilm biomass and microbial cells on titanium discs incubated up to 72 h. R89BS dispersal activity was addressed by measuring residual biomass of pre-formed biofilms after rhamnolipid treatment up to 24 h. Adhesion and proliferation of human primary osteoblasts on R89BS-coated titanium were evaluated by cell count and adenosine-triphosphate quantification, while cell differentiation was studied by measuring alkaline phosphatase activity and observing mineral deposition. Results showed that R89BS coating inhibited <i>S. oralis</i> biofilm formation by 80% at 72 h and dislodged 63–86% of pre-formed biofilms in 24 h according to concentration. No change in the adhesion of human osteoblasts was observed, whereas proliferation was reduced accompanied by an increase in cell differentiation. R89BS effectively counteracts <i>S. oralis</i> biofilm formation on titanium and preserves overall osteoblasts behavior representing a promising preventive strategy against biofilm-related peri-implant diseases. |
| first_indexed | 2024-03-10T22:40:01Z |
| format | Article |
| id | doaj.art-216e1564a3754f2983b93424a8614921 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T22:40:01Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-216e1564a3754f2983b93424a86149212023-11-19T11:06:35ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-09-0124181401410.3390/ijms241814014Rhamnolipid 89 Biosurfactant Is Effective against <i>Streptococcus oralis</i> Biofilm and Preserves Osteoblast Behavior: Perspectives in Dental ImplantologyErica Tambone0Chiara Ceresa1Alice Marchetti2Silvia Chiera3Adriano Anesi4Giandomenico Nollo5Iole Caola6Michela Bosetti7Letizia Fracchia8Paolo Ghensi9Francesco Tessarolo10Department of Industrial Engineering & BIOtech, University of Trento, 38123 Trento, ItalyDepartment of Pharmaceutical Sciences, Università del Piemonte Orientale “A. Avogadro”, 28100 Novara, ItalyDepartment of Pharmaceutical Sciences, Università del Piemonte Orientale “A. Avogadro”, 28100 Novara, ItalyDepartment of Industrial Engineering & BIOtech, University of Trento, 38123 Trento, ItalyDepartment of Laboratory Medicine, Azienda Provinciale per i Servizi Sanitari, 38122 Trento, ItalyDepartment of Industrial Engineering & BIOtech, University of Trento, 38123 Trento, ItalyDepartment of Laboratory Medicine, Azienda Provinciale per i Servizi Sanitari, 38122 Trento, ItalyDepartment of Pharmaceutical Sciences, Università del Piemonte Orientale “A. Avogadro”, 28100 Novara, ItalyDepartment of Pharmaceutical Sciences, Università del Piemonte Orientale “A. Avogadro”, 28100 Novara, ItalyDepartment CIBIO, University of Trento, 38123 Trento, ItalyDepartment of Industrial Engineering & BIOtech, University of Trento, 38123 Trento, ItalyBiofilm-related peri-implant diseases represent the major complication for osteointegrated dental implants, requiring complex treatments or implant removal. Microbial biosurfactants emerged as new antibiofilm coating agents for implantable devices thanks to their high biocompatibility. This study aimed to assess the efficacy of the rhamnolipid 89 biosurfactant (R89BS) in limiting <i>Streptococcus oralis</i> biofilm formation and dislodging sessile cells from medical grade titanium, but preserving adhesion and proliferation of human osteoblasts. The inhibitory activity of a R89BS coating on <i>S. oralis</i> biofilm formation was assayed by quantifying biofilm biomass and microbial cells on titanium discs incubated up to 72 h. R89BS dispersal activity was addressed by measuring residual biomass of pre-formed biofilms after rhamnolipid treatment up to 24 h. Adhesion and proliferation of human primary osteoblasts on R89BS-coated titanium were evaluated by cell count and adenosine-triphosphate quantification, while cell differentiation was studied by measuring alkaline phosphatase activity and observing mineral deposition. Results showed that R89BS coating inhibited <i>S. oralis</i> biofilm formation by 80% at 72 h and dislodged 63–86% of pre-formed biofilms in 24 h according to concentration. No change in the adhesion of human osteoblasts was observed, whereas proliferation was reduced accompanied by an increase in cell differentiation. R89BS effectively counteracts <i>S. oralis</i> biofilm formation on titanium and preserves overall osteoblasts behavior representing a promising preventive strategy against biofilm-related peri-implant diseases.https://www.mdpi.com/1422-0067/24/18/14014antibiofilm activitybiocompatibilitydental implantsdislodging actionmicrobial biosurfactantsosteoblasts |
| spellingShingle | Erica Tambone Chiara Ceresa Alice Marchetti Silvia Chiera Adriano Anesi Giandomenico Nollo Iole Caola Michela Bosetti Letizia Fracchia Paolo Ghensi Francesco Tessarolo Rhamnolipid 89 Biosurfactant Is Effective against <i>Streptococcus oralis</i> Biofilm and Preserves Osteoblast Behavior: Perspectives in Dental Implantology International Journal of Molecular Sciences antibiofilm activity biocompatibility dental implants dislodging action microbial biosurfactants osteoblasts |
| title | Rhamnolipid 89 Biosurfactant Is Effective against <i>Streptococcus oralis</i> Biofilm and Preserves Osteoblast Behavior: Perspectives in Dental Implantology |
| title_full | Rhamnolipid 89 Biosurfactant Is Effective against <i>Streptococcus oralis</i> Biofilm and Preserves Osteoblast Behavior: Perspectives in Dental Implantology |
| title_fullStr | Rhamnolipid 89 Biosurfactant Is Effective against <i>Streptococcus oralis</i> Biofilm and Preserves Osteoblast Behavior: Perspectives in Dental Implantology |
| title_full_unstemmed | Rhamnolipid 89 Biosurfactant Is Effective against <i>Streptococcus oralis</i> Biofilm and Preserves Osteoblast Behavior: Perspectives in Dental Implantology |
| title_short | Rhamnolipid 89 Biosurfactant Is Effective against <i>Streptococcus oralis</i> Biofilm and Preserves Osteoblast Behavior: Perspectives in Dental Implantology |
| title_sort | rhamnolipid 89 biosurfactant is effective against i streptococcus oralis i biofilm and preserves osteoblast behavior perspectives in dental implantology |
| topic | antibiofilm activity biocompatibility dental implants dislodging action microbial biosurfactants osteoblasts |
| url | https://www.mdpi.com/1422-0067/24/18/14014 |
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