Chemical profile of Nepeta cataria L. var. citriodora (Becker) essential oil and in vitro evaluation of biological activities
Essential oil (EO) obtained by hydrodistillation in a Clevenger-type apparatus from aerial parts of Nepeta cataria L. var. citriodora (Becker), cultivated in Serbia was subjected to gas chromatography-mass spectroscopy (GC-MS) to determine the composition. Furthermore, N. cataria var. citriodora es...
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| Format: | Article |
| Language: | English |
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University of Life Sciences in Lublin - Publishing House
2022-08-01
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| Series: | Acta Scientiarum Polonorum: Hortorum Cultus |
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| Online Access: | https://czasopisma.up.lublin.pl/index.php/asphc/article/view/4516 |
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| author | Milica Acimovic Vanja Seregelj Katarina Simić Ana Varga Lato Pezo Jelena Vulić Ivana Cabarkapa |
| author_facet | Milica Acimovic Vanja Seregelj Katarina Simić Ana Varga Lato Pezo Jelena Vulić Ivana Cabarkapa |
| author_sort | Milica Acimovic |
| collection | DOAJ |
| description |
Essential oil (EO) obtained by hydrodistillation in a Clevenger-type apparatus from aerial parts of Nepeta cataria L. var. citriodora (Becker), cultivated in Serbia was subjected to gas chromatography-mass spectroscopy (GC-MS) to determine the composition. Furthermore, N. cataria var. citriodora essential oil was
tested to determine its antimicrobial, antioxidant, antihyperglycemic and anti-inflammatory activities in vitro.
The antimicrobial activity was tested by broth microdilution method against 16 bacterial strains from American Type Culture Collection (ATCC). Four common tests for measuring in vitro antioxidant activity were used: 2, 2-diphenyl-1-picrylhydrazyl assay (DPPH), reducing power (RP), 2,2-azino-bis-3-ethylbenzothiazoline-
6-sulfonic acid (ABTS) and β-carotene bleaching assay (BCB). Antihyperglycemic activity was examined by using α-glucosidase inhibitory potential (AHgA), while anti-inflammatory activity (AIA) was determined by protein denaturation bioassay, using egg albumin. In total, 36 compounds were isolated and detected by GC-MS technique in N. cataria var. citriodora EO. The EO is mainly comprised of oxygenated monoterpenes (93.1%), and the main compounds were two monoterpenoid alcohols, nerol (38.5%) and geraniol (24.9%), followed by two aliphatic aldehyde, geranial (14.6%) and neral (11.0%). Antimicrobial activity of this EO shows growth inhibition of all tested bacteria strains, and exhibited good antioxidant, antihyperglycemic and anti-inflammatory activities. The EO obtained from N. cataria var. citriodora grown in Serbia shows valuable biological activity, indicating its potential for use as a supplement in everyday diet and as a natural preservative in food industry.
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| first_indexed | 2024-03-09T00:12:53Z |
| format | Article |
| id | doaj.art-216eef00fc95472bb1004f32eee8889d |
| institution | Directory Open Access Journal |
| issn | 1644-0692 2545-1405 |
| language | English |
| last_indexed | 2024-03-09T00:12:53Z |
| publishDate | 2022-08-01 |
| publisher | University of Life Sciences in Lublin - Publishing House |
| record_format | Article |
| series | Acta Scientiarum Polonorum: Hortorum Cultus |
| spelling | doaj.art-216eef00fc95472bb1004f32eee8889d2023-12-12T09:40:01ZengUniversity of Life Sciences in Lublin - Publishing HouseActa Scientiarum Polonorum: Hortorum Cultus1644-06922545-14052022-08-0121410.24326/asphc.2022.4.7Chemical profile of Nepeta cataria L. var. citriodora (Becker) essential oil and in vitro evaluation of biological activitiesMilica Acimovic0Vanja Seregelj1Katarina Simić2Ana Varga3Lato Pezo4Jelena Vulić5Ivana Cabarkapa6Institute of Field and Vegetable Crops Novi SadFaculty of Technology, University of Novi Sad, SerbiaInstitute of Chemistry, Technology and Metallurgy, University of Belgrade, SerbiaInstitute of Food Technology, University of Novi Sad, SerbiaInstitute of General and Physical Chemistry, University of Belgrade, SerbiaFaculty of Technology, University of Novi Sad, SerbiaInstitute of Food Technology, University of Novi Sad, Serbia Essential oil (EO) obtained by hydrodistillation in a Clevenger-type apparatus from aerial parts of Nepeta cataria L. var. citriodora (Becker), cultivated in Serbia was subjected to gas chromatography-mass spectroscopy (GC-MS) to determine the composition. Furthermore, N. cataria var. citriodora essential oil was tested to determine its antimicrobial, antioxidant, antihyperglycemic and anti-inflammatory activities in vitro. The antimicrobial activity was tested by broth microdilution method against 16 bacterial strains from American Type Culture Collection (ATCC). Four common tests for measuring in vitro antioxidant activity were used: 2, 2-diphenyl-1-picrylhydrazyl assay (DPPH), reducing power (RP), 2,2-azino-bis-3-ethylbenzothiazoline- 6-sulfonic acid (ABTS) and β-carotene bleaching assay (BCB). Antihyperglycemic activity was examined by using α-glucosidase inhibitory potential (AHgA), while anti-inflammatory activity (AIA) was determined by protein denaturation bioassay, using egg albumin. In total, 36 compounds were isolated and detected by GC-MS technique in N. cataria var. citriodora EO. The EO is mainly comprised of oxygenated monoterpenes (93.1%), and the main compounds were two monoterpenoid alcohols, nerol (38.5%) and geraniol (24.9%), followed by two aliphatic aldehyde, geranial (14.6%) and neral (11.0%). Antimicrobial activity of this EO shows growth inhibition of all tested bacteria strains, and exhibited good antioxidant, antihyperglycemic and anti-inflammatory activities. The EO obtained from N. cataria var. citriodora grown in Serbia shows valuable biological activity, indicating its potential for use as a supplement in everyday diet and as a natural preservative in food industry. https://czasopisma.up.lublin.pl/index.php/asphc/article/view/4516lemon catnipantimicrobial activityantioxidant activityantihyperglycemic activityanti-inflammatory activity |
| spellingShingle | Milica Acimovic Vanja Seregelj Katarina Simić Ana Varga Lato Pezo Jelena Vulić Ivana Cabarkapa Chemical profile of Nepeta cataria L. var. citriodora (Becker) essential oil and in vitro evaluation of biological activities Acta Scientiarum Polonorum: Hortorum Cultus lemon catnip antimicrobial activity antioxidant activity antihyperglycemic activity anti-inflammatory activity |
| title | Chemical profile of Nepeta cataria L. var. citriodora (Becker) essential oil and in vitro evaluation of biological activities |
| title_full | Chemical profile of Nepeta cataria L. var. citriodora (Becker) essential oil and in vitro evaluation of biological activities |
| title_fullStr | Chemical profile of Nepeta cataria L. var. citriodora (Becker) essential oil and in vitro evaluation of biological activities |
| title_full_unstemmed | Chemical profile of Nepeta cataria L. var. citriodora (Becker) essential oil and in vitro evaluation of biological activities |
| title_short | Chemical profile of Nepeta cataria L. var. citriodora (Becker) essential oil and in vitro evaluation of biological activities |
| title_sort | chemical profile of nepeta cataria l var citriodora becker essential oil and in vitro evaluation of biological activities |
| topic | lemon catnip antimicrobial activity antioxidant activity antihyperglycemic activity anti-inflammatory activity |
| url | https://czasopisma.up.lublin.pl/index.php/asphc/article/view/4516 |
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