Zataria multiflora Boiss. Essential Oil Induce Apoptosis in Two Human Colon Cancer Cell Lines (HCT116 & SW48)
Background: Zataria multiflora Boiss. is known by the common Persian name “Avishan-e-Shirazi”, is one of the best-known medicinal herbs belonging to the Labiatae (Lamiaceae) family. The aim of this study was to evaluate the anticancer effects and the underlying mechanisms of how Z. multiflora Boiss....
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2020-04-01
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| Series: | Iranian Journal of Public Health |
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| Online Access: | https://ijph.tums.ac.ir/index.php/ijph/article/view/12366 |
| _version_ | 1818564820223393792 |
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| author | Narges AHANI Mohammad Hossein SANGTARASH Majid ALIPOUR ESKANDANI Massoud HOUSHMAND |
| author_facet | Narges AHANI Mohammad Hossein SANGTARASH Majid ALIPOUR ESKANDANI Massoud HOUSHMAND |
| author_sort | Narges AHANI |
| collection | DOAJ |
| description | Background: Zataria multiflora Boiss. is known by the common Persian name “Avishan-e-Shirazi”, is one of the best-known medicinal herbs belonging to the Labiatae (Lamiaceae) family. The aim of this study was to evaluate the anticancer effects and the underlying mechanisms of how Z. multiflora Boiss., essential oil induced apoptosis in the human colorectal tumor cell lines (HCT116 & SW48).
Methods: This study was conducted in National Institute of Genetic Engineering and Biotechnology (NIGEB) (Tehran, Iran) from 2017 to 2019. The cytotoxicity of this essential oil was assessed by 3- (4,5-di-methylthiazol-2-yl)- 2,5-diphenyltetra-zolium bromide (MTT) assay, trypan blue exclusion, and colony formation assays. We assessed apoptosis and measure intracellular reactive oxygen species (ROS) by flow cytometry. Then gene expression was analyzed by Quantitative Real-Time RT-PCR.
Results: Z. multiflora Boiss., essential oil time- and dose-dependently inhibits cell proliferation and also induced apoptosis in both cell lines via UCP2-related mitochondrial pathway by the induction of intracellular ROS.
Conclusion: Z. multiflora Boiss., essential oil could be a good candidate for use as an inhibitor of the growth of colorectal tumor cells. |
| first_indexed | 2024-12-14T01:33:32Z |
| format | Article |
| id | doaj.art-216fef73e8df4bbd9c3155919fbf839f |
| institution | Directory Open Access Journal |
| issn | 2251-6085 2251-6093 |
| language | English |
| last_indexed | 2024-12-14T01:33:32Z |
| publishDate | 2020-04-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Public Health |
| spelling | doaj.art-216fef73e8df4bbd9c3155919fbf839f2022-12-21T23:21:57ZengTehran University of Medical SciencesIranian Journal of Public Health2251-60852251-60932020-04-01494Zataria multiflora Boiss. Essential Oil Induce Apoptosis in Two Human Colon Cancer Cell Lines (HCT116 & SW48)Narges AHANI0Mohammad Hossein SANGTARASH1Majid ALIPOUR ESKANDANI2Massoud HOUSHMAND3Department of Biology, Faculty of Sciences, University of Sistan and Baluchestan, Zahedan, IranDepartment of Biology, Faculty of Sciences, University of Sistan and Baluchestan, Zahedan, IranDepartment of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, University of Zabol, Zabol, IranDepartment of Medical Genetics, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran AND Research Center, Knowledge University, Erbil, Kurdistan Region, IraqBackground: Zataria multiflora Boiss. is known by the common Persian name “Avishan-e-Shirazi”, is one of the best-known medicinal herbs belonging to the Labiatae (Lamiaceae) family. The aim of this study was to evaluate the anticancer effects and the underlying mechanisms of how Z. multiflora Boiss., essential oil induced apoptosis in the human colorectal tumor cell lines (HCT116 & SW48). Methods: This study was conducted in National Institute of Genetic Engineering and Biotechnology (NIGEB) (Tehran, Iran) from 2017 to 2019. The cytotoxicity of this essential oil was assessed by 3- (4,5-di-methylthiazol-2-yl)- 2,5-diphenyltetra-zolium bromide (MTT) assay, trypan blue exclusion, and colony formation assays. We assessed apoptosis and measure intracellular reactive oxygen species (ROS) by flow cytometry. Then gene expression was analyzed by Quantitative Real-Time RT-PCR. Results: Z. multiflora Boiss., essential oil time- and dose-dependently inhibits cell proliferation and also induced apoptosis in both cell lines via UCP2-related mitochondrial pathway by the induction of intracellular ROS. Conclusion: Z. multiflora Boiss., essential oil could be a good candidate for use as an inhibitor of the growth of colorectal tumor cells.https://ijph.tums.ac.ir/index.php/ijph/article/view/12366Zataria multiflora Boiss.Essential oil;ApoptosisColon cancer |
| spellingShingle | Narges AHANI Mohammad Hossein SANGTARASH Majid ALIPOUR ESKANDANI Massoud HOUSHMAND Zataria multiflora Boiss. Essential Oil Induce Apoptosis in Two Human Colon Cancer Cell Lines (HCT116 & SW48) Iranian Journal of Public Health Zataria multiflora Boiss. Essential oil; Apoptosis Colon cancer |
| title | Zataria multiflora Boiss. Essential Oil Induce Apoptosis in Two Human Colon Cancer Cell Lines (HCT116 & SW48) |
| title_full | Zataria multiflora Boiss. Essential Oil Induce Apoptosis in Two Human Colon Cancer Cell Lines (HCT116 & SW48) |
| title_fullStr | Zataria multiflora Boiss. Essential Oil Induce Apoptosis in Two Human Colon Cancer Cell Lines (HCT116 & SW48) |
| title_full_unstemmed | Zataria multiflora Boiss. Essential Oil Induce Apoptosis in Two Human Colon Cancer Cell Lines (HCT116 & SW48) |
| title_short | Zataria multiflora Boiss. Essential Oil Induce Apoptosis in Two Human Colon Cancer Cell Lines (HCT116 & SW48) |
| title_sort | zataria multiflora boiss essential oil induce apoptosis in two human colon cancer cell lines hct116 sw48 |
| topic | Zataria multiflora Boiss. Essential oil; Apoptosis Colon cancer |
| url | https://ijph.tums.ac.ir/index.php/ijph/article/view/12366 |
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