Key auxin response factor (ARF) genes constraining wheat tillering of mutant dmc
Tillering ability is a key agronomy trait for wheat (Triticum aestivum L.) production. Studies on a dwarf monoculm wheat mutant (dmc) showed that ARF11 played an important role in tillering of wheat. In this study, a total of 67 ARF family members were identified and clustered to two main classes wi...
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PeerJ Inc.
2021-09-01
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| Online Access: | https://peerj.com/articles/12221.pdf |
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| author | Junchang Li Yumei Jiang Jing Zhang Yongjing Ni Zhixin Jiao Huijuan Li Ting Wang Peipei Zhang Wenlong Guo Lei Li Hongjie Liu Hairong Zhang Qiaoyun Li Jishan Niu |
| author_facet | Junchang Li Yumei Jiang Jing Zhang Yongjing Ni Zhixin Jiao Huijuan Li Ting Wang Peipei Zhang Wenlong Guo Lei Li Hongjie Liu Hairong Zhang Qiaoyun Li Jishan Niu |
| author_sort | Junchang Li |
| collection | DOAJ |
| description | Tillering ability is a key agronomy trait for wheat (Triticum aestivum L.) production. Studies on a dwarf monoculm wheat mutant (dmc) showed that ARF11 played an important role in tillering of wheat. In this study, a total of 67 ARF family members were identified and clustered to two main classes with four subgroups based on their protein structures. The promoter regions of T. aestivum ARF (TaARF) genes contain a large number of cis-acting elements closely related to plant growth and development, and hormone response. The segmental duplication events occurred commonly and played a major role in the expansion of TaARFs. The gene collinearity degrees of the ARFs between wheat and other grasses, rice and maize, were significantly high. The evolution distances among TaARFs determine their expression profiles, such as homoeologous genes have similar expression profiles, like TaARF4-3A-1, TaARF4-3A-2 and their homoeologous genes. The expression profiles of TaARFs in various tissues or organs indicated TaARF3, TaARF4, TaARF9 and TaARF22 and their homoeologous genes played basic roles during wheat development. TaARF4, TaARF9, TaARF12, TaARF15, TaARF17, TaARF21, TaARF25 and their homoeologous genes probably played basic roles in tiller development. qRT-PCR analyses of 20 representative TaARF genes revealed that the abnormal expressions of TaARF11 and TaARF14 were major causes constraining the tillering of dmc. Indole-3-acetic acid (IAA) contents in dmc were significantly less than that in Guomai 301 at key tillering stages. Exogenous IAA application significantly promoted wheat tillering, and affected the transcriptions of TaARFs. These data suggested that TaARFs as well as IAA signaling were involved in controlling wheat tillering. This study provided valuable clues for functional characterization of ARFs in wheat. |
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| language | English |
| last_indexed | 2024-03-09T08:06:13Z |
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| spelling | doaj.art-217275019e76452684622abc5cf76f3b2023-12-02T23:47:46ZengPeerJ Inc.PeerJ2167-83592021-09-019e1222110.7717/peerj.12221Key auxin response factor (ARF) genes constraining wheat tillering of mutant dmcJunchang Li0Yumei Jiang1Jing Zhang2Yongjing Ni3Zhixin Jiao4Huijuan Li5Ting Wang6Peipei Zhang7Wenlong Guo8Lei Li9Hongjie Liu10Hairong Zhang11Qiaoyun Li12Jishan Niu13National Centre of Engineering and Technological Research for Wheat/National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou, Henan, ChinaNational Centre of Engineering and Technological Research for Wheat/National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou, Henan, ChinaNational Centre of Engineering and Technological Research for Wheat/National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou, Henan, ChinaShangqiu Academy of Agricultural and Forestry Sciences, Shangqiu, Henan, ChinaNational Centre of Engineering and Technological Research for Wheat/National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou, Henan, ChinaNational Centre of Engineering and Technological Research for Wheat/National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou, Henan, ChinaNational Centre of Engineering and Technological Research for Wheat/National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou, Henan, ChinaNational Centre of Engineering and Technological Research for Wheat/National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou, Henan, ChinaNational Centre of Engineering and Technological Research for Wheat/National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou, Henan, ChinaNational Centre of Engineering and Technological Research for Wheat/National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou, Henan, ChinaShangqiu Academy of Agricultural and Forestry Sciences, Shangqiu, Henan, ChinaCollege of Life Sciences, Henan Agricultural University, Zhengzhou, Henan, ChinaNational Centre of Engineering and Technological Research for Wheat/National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou, Henan, ChinaNational Centre of Engineering and Technological Research for Wheat/National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou, Henan, ChinaTillering ability is a key agronomy trait for wheat (Triticum aestivum L.) production. Studies on a dwarf monoculm wheat mutant (dmc) showed that ARF11 played an important role in tillering of wheat. In this study, a total of 67 ARF family members were identified and clustered to two main classes with four subgroups based on their protein structures. The promoter regions of T. aestivum ARF (TaARF) genes contain a large number of cis-acting elements closely related to plant growth and development, and hormone response. The segmental duplication events occurred commonly and played a major role in the expansion of TaARFs. The gene collinearity degrees of the ARFs between wheat and other grasses, rice and maize, were significantly high. The evolution distances among TaARFs determine their expression profiles, such as homoeologous genes have similar expression profiles, like TaARF4-3A-1, TaARF4-3A-2 and their homoeologous genes. The expression profiles of TaARFs in various tissues or organs indicated TaARF3, TaARF4, TaARF9 and TaARF22 and their homoeologous genes played basic roles during wheat development. TaARF4, TaARF9, TaARF12, TaARF15, TaARF17, TaARF21, TaARF25 and their homoeologous genes probably played basic roles in tiller development. qRT-PCR analyses of 20 representative TaARF genes revealed that the abnormal expressions of TaARF11 and TaARF14 were major causes constraining the tillering of dmc. Indole-3-acetic acid (IAA) contents in dmc were significantly less than that in Guomai 301 at key tillering stages. Exogenous IAA application significantly promoted wheat tillering, and affected the transcriptions of TaARFs. These data suggested that TaARFs as well as IAA signaling were involved in controlling wheat tillering. This study provided valuable clues for functional characterization of ARFs in wheat.https://peerj.com/articles/12221.pdfWheat (Triticum aestivum L.)TilleringAuxin response factorExpression profilesIAA |
| spellingShingle | Junchang Li Yumei Jiang Jing Zhang Yongjing Ni Zhixin Jiao Huijuan Li Ting Wang Peipei Zhang Wenlong Guo Lei Li Hongjie Liu Hairong Zhang Qiaoyun Li Jishan Niu Key auxin response factor (ARF) genes constraining wheat tillering of mutant dmc PeerJ Wheat (Triticum aestivum L.) Tillering Auxin response factor Expression profiles IAA |
| title | Key auxin response factor (ARF) genes constraining wheat tillering of mutant dmc |
| title_full | Key auxin response factor (ARF) genes constraining wheat tillering of mutant dmc |
| title_fullStr | Key auxin response factor (ARF) genes constraining wheat tillering of mutant dmc |
| title_full_unstemmed | Key auxin response factor (ARF) genes constraining wheat tillering of mutant dmc |
| title_short | Key auxin response factor (ARF) genes constraining wheat tillering of mutant dmc |
| title_sort | key auxin response factor arf genes constraining wheat tillering of mutant dmc |
| topic | Wheat (Triticum aestivum L.) Tillering Auxin response factor Expression profiles IAA |
| url | https://peerj.com/articles/12221.pdf |
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