Circovirus Hepatitis Infection in Heart-Lung Transplant Patient, France
In March 2022, a 61-year-old woman in France who had received a heart-lung transplant sought treatment with chronic hepatitis mainly characterized by increased liver enzymes. After ruling out common etiologies, we used metagenomic next-generation sequencing to analyze a liver biopsy sample and iden...
| Main Authors: | , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Centers for Disease Control and Prevention
2023-02-01
|
| Series: | Emerging Infectious Diseases |
| Subjects: | |
| Online Access: | https://wwwnc.cdc.gov/eid/article/29/2/22-1468_article |
| _version_ | 1828056085731213312 |
|---|---|
| author | Philippe Pérot Jacques Fourgeaud Claire Rouzaud Béatrice Regnault Nicolas Da Rocha Hélène Fontaine Jérôme Le Pavec Samuel Dolidon Margaux Garzaro Delphine Chrétien Guillaume Morcrette Thierry Jo Molina Agnès Ferroni Marianne Leruez-Ville Olivier Lortholary Anne Jamet Marc Eloit |
| author_facet | Philippe Pérot Jacques Fourgeaud Claire Rouzaud Béatrice Regnault Nicolas Da Rocha Hélène Fontaine Jérôme Le Pavec Samuel Dolidon Margaux Garzaro Delphine Chrétien Guillaume Morcrette Thierry Jo Molina Agnès Ferroni Marianne Leruez-Ville Olivier Lortholary Anne Jamet Marc Eloit |
| author_sort | Philippe Pérot |
| collection | DOAJ |
| description |
In March 2022, a 61-year-old woman in France who had received a heart-lung transplant sought treatment with chronic hepatitis mainly characterized by increased liver enzymes. After ruling out common etiologies, we used metagenomic next-generation sequencing to analyze a liver biopsy sample and identified an unknown species of circovirus, tentatively named human circovirus 1 (HCirV-1). We found no other viral or bacterial sequences. HCirV-1 shared 70% amino acid identity with the closest known viral sequences. The viral genome was undetectable in blood samples from 2017–2019, then became detectable at low levels in September 2020 and peaked at very high titers (1010 genome copies/mL) in January 2022. In March 2022, we found >108 genome copies/g or mL in the liver and blood, concomitant with hepatic cytolysis. We detected HCirV-1 transcripts in 2% of hepatocytes, demonstrating viral replication and supporting the role of HCirV-1 in liver damage.
|
| first_indexed | 2024-04-10T20:48:49Z |
| format | Article |
| id | doaj.art-2173309472bd4b91b55fb8f797487f24 |
| institution | Directory Open Access Journal |
| issn | 1080-6040 1080-6059 |
| language | English |
| last_indexed | 2024-04-10T20:48:49Z |
| publishDate | 2023-02-01 |
| publisher | Centers for Disease Control and Prevention |
| record_format | Article |
| series | Emerging Infectious Diseases |
| spelling | doaj.art-2173309472bd4b91b55fb8f797487f242023-01-23T18:21:48ZengCenters for Disease Control and PreventionEmerging Infectious Diseases1080-60401080-60592023-02-0129228629310.3201/eid2902.221468Circovirus Hepatitis Infection in Heart-Lung Transplant Patient, FrancePhilippe PérotJacques FourgeaudClaire RouzaudBéatrice RegnaultNicolas Da RochaHélène FontaineJérôme Le PavecSamuel DolidonMargaux GarzaroDelphine ChrétienGuillaume MorcretteThierry Jo MolinaAgnès FerroniMarianne Leruez-VilleOlivier LortholaryAnne JametMarc Eloit In March 2022, a 61-year-old woman in France who had received a heart-lung transplant sought treatment with chronic hepatitis mainly characterized by increased liver enzymes. After ruling out common etiologies, we used metagenomic next-generation sequencing to analyze a liver biopsy sample and identified an unknown species of circovirus, tentatively named human circovirus 1 (HCirV-1). We found no other viral or bacterial sequences. HCirV-1 shared 70% amino acid identity with the closest known viral sequences. The viral genome was undetectable in blood samples from 2017–2019, then became detectable at low levels in September 2020 and peaked at very high titers (1010 genome copies/mL) in January 2022. In March 2022, we found >108 genome copies/g or mL in the liver and blood, concomitant with hepatic cytolysis. We detected HCirV-1 transcripts in 2% of hepatocytes, demonstrating viral replication and supporting the role of HCirV-1 in liver damage. https://wwwnc.cdc.gov/eid/article/29/2/22-1468_articlehepatitiscircovirusessolid organ transplantsmetagenomic next-generation sequencingmNGSviruses |
| spellingShingle | Philippe Pérot Jacques Fourgeaud Claire Rouzaud Béatrice Regnault Nicolas Da Rocha Hélène Fontaine Jérôme Le Pavec Samuel Dolidon Margaux Garzaro Delphine Chrétien Guillaume Morcrette Thierry Jo Molina Agnès Ferroni Marianne Leruez-Ville Olivier Lortholary Anne Jamet Marc Eloit Circovirus Hepatitis Infection in Heart-Lung Transplant Patient, France Emerging Infectious Diseases hepatitis circoviruses solid organ transplants metagenomic next-generation sequencing mNGS viruses |
| title | Circovirus Hepatitis Infection in Heart-Lung Transplant Patient, France |
| title_full | Circovirus Hepatitis Infection in Heart-Lung Transplant Patient, France |
| title_fullStr | Circovirus Hepatitis Infection in Heart-Lung Transplant Patient, France |
| title_full_unstemmed | Circovirus Hepatitis Infection in Heart-Lung Transplant Patient, France |
| title_short | Circovirus Hepatitis Infection in Heart-Lung Transplant Patient, France |
| title_sort | circovirus hepatitis infection in heart lung transplant patient france |
| topic | hepatitis circoviruses solid organ transplants metagenomic next-generation sequencing mNGS viruses |
| url | https://wwwnc.cdc.gov/eid/article/29/2/22-1468_article |
| work_keys_str_mv | AT philippeperot circovirushepatitisinfectioninheartlungtransplantpatientfrance AT jacquesfourgeaud circovirushepatitisinfectioninheartlungtransplantpatientfrance AT clairerouzaud circovirushepatitisinfectioninheartlungtransplantpatientfrance AT beatriceregnault circovirushepatitisinfectioninheartlungtransplantpatientfrance AT nicolasdarocha circovirushepatitisinfectioninheartlungtransplantpatientfrance AT helenefontaine circovirushepatitisinfectioninheartlungtransplantpatientfrance AT jeromelepavec circovirushepatitisinfectioninheartlungtransplantpatientfrance AT samueldolidon circovirushepatitisinfectioninheartlungtransplantpatientfrance AT margauxgarzaro circovirushepatitisinfectioninheartlungtransplantpatientfrance AT delphinechretien circovirushepatitisinfectioninheartlungtransplantpatientfrance AT guillaumemorcrette circovirushepatitisinfectioninheartlungtransplantpatientfrance AT thierryjomolina circovirushepatitisinfectioninheartlungtransplantpatientfrance AT agnesferroni circovirushepatitisinfectioninheartlungtransplantpatientfrance AT marianneleruezville circovirushepatitisinfectioninheartlungtransplantpatientfrance AT olivierlortholary circovirushepatitisinfectioninheartlungtransplantpatientfrance AT annejamet circovirushepatitisinfectioninheartlungtransplantpatientfrance AT marceloit circovirushepatitisinfectioninheartlungtransplantpatientfrance |