Progesterone Enhanced Remyelination in the Mouse Corpus Callosum After Cuprizone Induced Demyelination
Background: Progesterone as a sex steroid hormone is thought to affect and prevent demyelination, but its role in promoting myelin repair is far less investigated. In this study, remyelinating potential of progesterone in corpus callosum was evaluated on an experimental model of MS. Methods: In th...
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| Format: | Article |
| Language: | English |
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Shiraz University of Medical Sciences
2015-11-01
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| Series: | Iranian Journal of Medical Sciences |
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| Online Access: | http://ijms.sums.ac.ir/index.php/IJMS/article/view/191 |
| _version_ | 1828741542157746176 |
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| author | Iraj Ragerdi Kashani Azim Hedayatpour Parichehr Pasbakhsh Laya Kafami Behzad Khallaghi Fatemeh Malek |
| author_facet | Iraj Ragerdi Kashani Azim Hedayatpour Parichehr Pasbakhsh Laya Kafami Behzad Khallaghi Fatemeh Malek |
| author_sort | Iraj Ragerdi Kashani |
| collection | DOAJ |
| description | Background: Progesterone as a sex steroid hormone is thought to affect and prevent demyelination, but its role in promoting myelin repair is far less investigated. In this study, remyelinating potential of progesterone in corpus callosum was evaluated on an experimental model of MS.
Methods: In this experimental study, adult male C57BL/6 mice were fed with 0.2% (w/w) cuprizone in ground breeder chow ad libitum for 6 weeks. At day zero, after cuprizone removal, mice were divided randomly into two groups: (a) placebo group, which received saline pellet implant, (b) progesterone group, which received progesterone pellet implant. Some mice of the same age were fed with their normal diet to serve as the healthy control group. Two weeks after progesterone administration, Myelin content was assessed by Luxol-fast blue staining. The myelin basic protein (MBP) and proteolipid protein (PLP) expression were assessed using Western blot analysis and the changes in the number of oligodendrocytes and oligodendroglial progenitor cells were assessed by immunohistochemistry (IHC) and flow cytometry.
Results: Luxol-fast blue staining revealed enhanced remyelination in the progesterone group when compared with the placebo group. Densitometry measurements of immunoblots demonstrated that MBP and PLP proteins contents were significantly increased in the progesterone group compared with the placebo group. Flow cytometry and IHC analysis showed increases in Olig2 and O4 cells in the progesterone group compared with the placebo group.
Conclusion: Overall, our results indicate that progesterone treatment can stimulate myelin production and that it may provide a feasible and practical way for remyelination in diseases such as multiple sclerosis. |
| first_indexed | 2024-04-13T01:02:58Z |
| format | Article |
| id | doaj.art-217489f197654372816998368c2caa7b |
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| issn | 0253-0716 1735-3688 |
| language | English |
| last_indexed | 2024-04-13T01:02:58Z |
| publishDate | 2015-11-01 |
| publisher | Shiraz University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Medical Sciences |
| spelling | doaj.art-217489f197654372816998368c2caa7b2022-12-22T03:09:26ZengShiraz University of Medical SciencesIranian Journal of Medical Sciences0253-07161735-36882015-11-01406507514Progesterone Enhanced Remyelination in the Mouse Corpus Callosum After Cuprizone Induced DemyelinationIraj Ragerdi Kashani0Azim Hedayatpour1Parichehr Pasbakhsh2Laya Kafami3Behzad Khallaghi4Fatemeh Malek5Department of Anatomical Sciences, School of Medicine, Tehran University of Medical Sciences, Tehran, IranDepartment of Anatomical Sciences, School of Medicine, Tehran University of Medical Sciences, Tehran, IranDepartment of Anatomical Sciences, School of Medicine, Tehran University of Medical Sciences, Tehran, IranPathobiology Department, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran; and Shefa Neurosciences Research Center, Tehran, IranShefa Neurosciences Research Center, Tehran, IranDepartment of Anatomical Sciences, School of Medicine, Tehran University of Medical Sciences, Tehran, IranBackground: Progesterone as a sex steroid hormone is thought to affect and prevent demyelination, but its role in promoting myelin repair is far less investigated. In this study, remyelinating potential of progesterone in corpus callosum was evaluated on an experimental model of MS. Methods: In this experimental study, adult male C57BL/6 mice were fed with 0.2% (w/w) cuprizone in ground breeder chow ad libitum for 6 weeks. At day zero, after cuprizone removal, mice were divided randomly into two groups: (a) placebo group, which received saline pellet implant, (b) progesterone group, which received progesterone pellet implant. Some mice of the same age were fed with their normal diet to serve as the healthy control group. Two weeks after progesterone administration, Myelin content was assessed by Luxol-fast blue staining. The myelin basic protein (MBP) and proteolipid protein (PLP) expression were assessed using Western blot analysis and the changes in the number of oligodendrocytes and oligodendroglial progenitor cells were assessed by immunohistochemistry (IHC) and flow cytometry. Results: Luxol-fast blue staining revealed enhanced remyelination in the progesterone group when compared with the placebo group. Densitometry measurements of immunoblots demonstrated that MBP and PLP proteins contents were significantly increased in the progesterone group compared with the placebo group. Flow cytometry and IHC analysis showed increases in Olig2 and O4 cells in the progesterone group compared with the placebo group. Conclusion: Overall, our results indicate that progesterone treatment can stimulate myelin production and that it may provide a feasible and practical way for remyelination in diseases such as multiple sclerosis.http://ijms.sums.ac.ir/index.php/IJMS/article/view/191Multiple sclerosisRemyelinationProgesterone |
| spellingShingle | Iraj Ragerdi Kashani Azim Hedayatpour Parichehr Pasbakhsh Laya Kafami Behzad Khallaghi Fatemeh Malek Progesterone Enhanced Remyelination in the Mouse Corpus Callosum After Cuprizone Induced Demyelination Iranian Journal of Medical Sciences Multiple sclerosis Remyelination Progesterone |
| title | Progesterone Enhanced Remyelination in the Mouse Corpus Callosum After Cuprizone Induced Demyelination |
| title_full | Progesterone Enhanced Remyelination in the Mouse Corpus Callosum After Cuprizone Induced Demyelination |
| title_fullStr | Progesterone Enhanced Remyelination in the Mouse Corpus Callosum After Cuprizone Induced Demyelination |
| title_full_unstemmed | Progesterone Enhanced Remyelination in the Mouse Corpus Callosum After Cuprizone Induced Demyelination |
| title_short | Progesterone Enhanced Remyelination in the Mouse Corpus Callosum After Cuprizone Induced Demyelination |
| title_sort | progesterone enhanced remyelination in the mouse corpus callosum after cuprizone induced demyelination |
| topic | Multiple sclerosis Remyelination Progesterone |
| url | http://ijms.sums.ac.ir/index.php/IJMS/article/view/191 |
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