Advances and Trends in miRNA Analysis Using DNAzyme-Based Biosensors
miRNAs are endogenous small, non-coding RNA molecules that function in post-transcriptional regulation of gene expression. Because miRNA plays a pivotal role in maintaining the intracellular environment, and abnormal expression has been found in many cancer diseases, detection of miRNA as a biomarke...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-08-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/13/9/856 |
| _version_ | 1827726911783043072 |
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| author | Minhyuk Lee Seungjae Kang Sungjee Kim Nokyoung Park |
| author_facet | Minhyuk Lee Seungjae Kang Sungjee Kim Nokyoung Park |
| author_sort | Minhyuk Lee |
| collection | DOAJ |
| description | miRNAs are endogenous small, non-coding RNA molecules that function in post-transcriptional regulation of gene expression. Because miRNA plays a pivotal role in maintaining the intracellular environment, and abnormal expression has been found in many cancer diseases, detection of miRNA as a biomarker is important for early diagnosis of disease and study of miRNA function. However, because miRNA is present in extremely low concentrations in cells and many types of miRNAs with similar sequences are mixed, traditional gene detection methods are not suitable for miRNA detection. Therefore, in order to overcome this limitation, a signal amplification process is essential for high sensitivity. In particular, enzyme-free signal amplification systems such as DNAzyme systems have been developed for miRNA analysis with high specificity. DNAzymes have the advantage of being more stable in the physiological environment than enzymes, easy to chemically synthesize, and biocompatible. In this review, we summarize and introduce the methods using DNAzyme-based biosensors, especially with regard to various signal amplification methods for high sensitivity and strategies for improving detection specificity. We also discuss the current challenges and trends of these DNAzyme-based biosensors. |
| first_indexed | 2024-03-10T22:59:04Z |
| format | Article |
| id | doaj.art-2174ec794e8946baad740d8a808a3ab3 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-10T22:59:04Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-2174ec794e8946baad740d8a808a3ab32023-11-19T09:47:17ZengMDPI AGBiosensors2079-63742023-08-0113985610.3390/bios13090856Advances and Trends in miRNA Analysis Using DNAzyme-Based BiosensorsMinhyuk Lee0Seungjae Kang1Sungjee Kim2Nokyoung Park3Department of Chemistry, Pohang University of Science and Technology, Pohang 37673, Republic of KoreaDepartment of Chemistry and the Natural Science Research Institute, Myongji University, 116 Myongji-ro, Yongin-si 17058, Republic of KoreaDepartment of Chemistry, Pohang University of Science and Technology, Pohang 37673, Republic of KoreaDepartment of Chemistry and the Natural Science Research Institute, Myongji University, 116 Myongji-ro, Yongin-si 17058, Republic of KoreamiRNAs are endogenous small, non-coding RNA molecules that function in post-transcriptional regulation of gene expression. Because miRNA plays a pivotal role in maintaining the intracellular environment, and abnormal expression has been found in many cancer diseases, detection of miRNA as a biomarker is important for early diagnosis of disease and study of miRNA function. However, because miRNA is present in extremely low concentrations in cells and many types of miRNAs with similar sequences are mixed, traditional gene detection methods are not suitable for miRNA detection. Therefore, in order to overcome this limitation, a signal amplification process is essential for high sensitivity. In particular, enzyme-free signal amplification systems such as DNAzyme systems have been developed for miRNA analysis with high specificity. DNAzymes have the advantage of being more stable in the physiological environment than enzymes, easy to chemically synthesize, and biocompatible. In this review, we summarize and introduce the methods using DNAzyme-based biosensors, especially with regard to various signal amplification methods for high sensitivity and strategies for improving detection specificity. We also discuss the current challenges and trends of these DNAzyme-based biosensors.https://www.mdpi.com/2079-6374/13/9/856miRNADNAzymebiosensor |
| spellingShingle | Minhyuk Lee Seungjae Kang Sungjee Kim Nokyoung Park Advances and Trends in miRNA Analysis Using DNAzyme-Based Biosensors Biosensors miRNA DNAzyme biosensor |
| title | Advances and Trends in miRNA Analysis Using DNAzyme-Based Biosensors |
| title_full | Advances and Trends in miRNA Analysis Using DNAzyme-Based Biosensors |
| title_fullStr | Advances and Trends in miRNA Analysis Using DNAzyme-Based Biosensors |
| title_full_unstemmed | Advances and Trends in miRNA Analysis Using DNAzyme-Based Biosensors |
| title_short | Advances and Trends in miRNA Analysis Using DNAzyme-Based Biosensors |
| title_sort | advances and trends in mirna analysis using dnazyme based biosensors |
| topic | miRNA DNAzyme biosensor |
| url | https://www.mdpi.com/2079-6374/13/9/856 |
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