Hepatitis B virus genotypes associated with pregnant women in Northern Thailand
Background: Mother-to-child transmission of hepatitis B virus (HBV) is the major route of transmission causing persistent infection. The prevalence of HBV infection and HBV genotypes found in different geographical areas varies from country to country. Therefore, this study was conducted to identify...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-03-01
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| Series: | Journal of Infection and Public Health |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1876034123004628 |
| _version_ | 1797304604659023872 |
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| author | Sirinart Sirilert Pattara Khamrin Kattareeya Kumthip Rungnapa Malasao Theera Tongsong Niwat Maneekarn |
| author_facet | Sirinart Sirilert Pattara Khamrin Kattareeya Kumthip Rungnapa Malasao Theera Tongsong Niwat Maneekarn |
| author_sort | Sirinart Sirilert |
| collection | DOAJ |
| description | Background: Mother-to-child transmission of hepatitis B virus (HBV) is the major route of transmission causing persistent infection. The prevalence of HBV infection and HBV genotypes found in different geographical areas varies from country to country. Therefore, this study was conducted to identify the HBV genotypes in HBV-infected pregnant women in Northern Thailand. Methods: Stored blood samples that were collected from 145 HBsAg-positive pregnant women who gave birth at Maharaj Nakorn Chiang Mai Hospital, Chiang Mai, Thailand from 2017 to 2020 were analyzed. The partial nucleotide sequence of the S gene of HBV was amplified by nested PCR and sequenced. All sequences were analyzed phylogenetically together with the reference strains to define the HBV genotypes. Results: A total of 31 blood samples from 145 HBsAg-positive pregnant women were positive for HBV by nested PCR. The detected HBV strains were identified as presumptive subgenotypes C1 (77.4%; 24/31), B9 (9.7%; 3/31), C2 (3.2%; 1/31), B2 (3.2%; 1/31), B4 (3.2%; 1/31), and presumptive B4/C2 recombinant subgenotype (3.2%; 1/31). Conclusions: The findings revealed that presumptive subgenotype C1 was the most common subgenotype circulating in pregnant women in Northern Thailand and accounted for 77.4% of cases, followed by presumptive subgenotypes B9, C2, B2, and B4. Furthermore, this study reported, for the first time in Thailand, the HBV genotypes and presumptive subgenotypes, particularly subgenotype B9 circulating in pregnant women. |
| first_indexed | 2024-03-08T00:12:49Z |
| format | Article |
| id | doaj.art-2175b2a64c2d4ad08727ac2cd05c9344 |
| institution | Directory Open Access Journal |
| issn | 1876-0341 |
| language | English |
| last_indexed | 2024-03-08T00:12:49Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Infection and Public Health |
| spelling | doaj.art-2175b2a64c2d4ad08727ac2cd05c93442024-02-17T06:37:24ZengElsevierJournal of Infection and Public Health1876-03412024-03-01173406411Hepatitis B virus genotypes associated with pregnant women in Northern ThailandSirinart Sirilert0Pattara Khamrin1Kattareeya Kumthip2Rungnapa Malasao3Theera Tongsong4Niwat Maneekarn5Department of Obstetrics and Gynecology, Faculty of Medicine, Chiang Mai University, Chiang Mai, ThailandDepartment of Microbiology, Faculty of Medicine, and Emerging and Re-emerging Diarrheal Viruses Cluster, Chiang Mai University, Chiang Mai, ThailandDepartment of Microbiology, Faculty of Medicine, and Emerging and Re-emerging Diarrheal Viruses Cluster, Chiang Mai University, Chiang Mai, ThailandDepartment of Community Medicine, Faculty of Medicine, Chiang Mai University, Chiang Mai, ThailandDepartment of Obstetrics and Gynecology, Faculty of Medicine, Chiang Mai University, Chiang Mai, ThailandDepartment of Microbiology, Faculty of Medicine, and Emerging and Re-emerging Diarrheal Viruses Cluster, Chiang Mai University, Chiang Mai, Thailand; Correspondence to: Department of Microbiology, Faculty of Medicine, Chiang Mai University, 110 Inthawarorot Road, Si Phum, Muang, Chiang Mai 50200, Thailand.Background: Mother-to-child transmission of hepatitis B virus (HBV) is the major route of transmission causing persistent infection. The prevalence of HBV infection and HBV genotypes found in different geographical areas varies from country to country. Therefore, this study was conducted to identify the HBV genotypes in HBV-infected pregnant women in Northern Thailand. Methods: Stored blood samples that were collected from 145 HBsAg-positive pregnant women who gave birth at Maharaj Nakorn Chiang Mai Hospital, Chiang Mai, Thailand from 2017 to 2020 were analyzed. The partial nucleotide sequence of the S gene of HBV was amplified by nested PCR and sequenced. All sequences were analyzed phylogenetically together with the reference strains to define the HBV genotypes. Results: A total of 31 blood samples from 145 HBsAg-positive pregnant women were positive for HBV by nested PCR. The detected HBV strains were identified as presumptive subgenotypes C1 (77.4%; 24/31), B9 (9.7%; 3/31), C2 (3.2%; 1/31), B2 (3.2%; 1/31), B4 (3.2%; 1/31), and presumptive B4/C2 recombinant subgenotype (3.2%; 1/31). Conclusions: The findings revealed that presumptive subgenotype C1 was the most common subgenotype circulating in pregnant women in Northern Thailand and accounted for 77.4% of cases, followed by presumptive subgenotypes B9, C2, B2, and B4. Furthermore, this study reported, for the first time in Thailand, the HBV genotypes and presumptive subgenotypes, particularly subgenotype B9 circulating in pregnant women.http://www.sciencedirect.com/science/article/pii/S1876034123004628Hepatitis B virusEpidemiologyPregnancyNorthern ThailandGenotype |
| spellingShingle | Sirinart Sirilert Pattara Khamrin Kattareeya Kumthip Rungnapa Malasao Theera Tongsong Niwat Maneekarn Hepatitis B virus genotypes associated with pregnant women in Northern Thailand Journal of Infection and Public Health Hepatitis B virus Epidemiology Pregnancy Northern Thailand Genotype |
| title | Hepatitis B virus genotypes associated with pregnant women in Northern Thailand |
| title_full | Hepatitis B virus genotypes associated with pregnant women in Northern Thailand |
| title_fullStr | Hepatitis B virus genotypes associated with pregnant women in Northern Thailand |
| title_full_unstemmed | Hepatitis B virus genotypes associated with pregnant women in Northern Thailand |
| title_short | Hepatitis B virus genotypes associated with pregnant women in Northern Thailand |
| title_sort | hepatitis b virus genotypes associated with pregnant women in northern thailand |
| topic | Hepatitis B virus Epidemiology Pregnancy Northern Thailand Genotype |
| url | http://www.sciencedirect.com/science/article/pii/S1876034123004628 |
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