Synthesis and Characterization of Polymer-Based Coatings Modified with Bioactive Ceramic and Bovine Serum Albumin
This study involves the synthesis of hydroxyapatite and describes the preparation and characterization of polymer coatings based on poly(ethylene glycol) diacrylate and poly(ethylene glycol) and modified with bovine serum albumin and hydroxyapatite. Hydroxyapatite was obtained by wet chemical synthe...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-03-01
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| Series: | Journal of Functional Biomaterials |
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| Online Access: | https://www.mdpi.com/2079-4983/12/2/21 |
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| author | Wioletta Florkiewicz Dagmara Słota Angelika Placek Klaudia Pluta Bożena Tyliszczak Timothy E. L. Douglas Agnieszka Sobczak-Kupiec |
| author_facet | Wioletta Florkiewicz Dagmara Słota Angelika Placek Klaudia Pluta Bożena Tyliszczak Timothy E. L. Douglas Agnieszka Sobczak-Kupiec |
| author_sort | Wioletta Florkiewicz |
| collection | DOAJ |
| description | This study involves the synthesis of hydroxyapatite and describes the preparation and characterization of polymer coatings based on poly(ethylene glycol) diacrylate and poly(ethylene glycol) and modified with bovine serum albumin and hydroxyapatite. Hydroxyapatite was obtained by wet chemical synthesis and characterized by X-ray diffraction and FTIR spectroscopy, and its Ca/P molar ratio was determined (1.69 ± 0.08). The ceramic and bovine serum albumin were used in the preparation of composite materials with the polymeric matrix. The chemical composition of coatings was characterized with FTIR spectroscopy, and their morphology was recorded with SEM imaging. Moreover, the measurements of surface roughness parameters and stereometric research were performed. The prepared coatings were subjected to in vitro studies in simulated body fluid and artificial saliva. Changes in chemical composition and morphology after immersion were examined with FTIR spectroscopy and SEM imaging. Based on the conducted research, it can be stated that applied modifiers promote the biomineralization process. The roughness analysis confirmed prepared materials were characterized by the micrometer-scale topography. The materials morphology and roughness, and the morphology of the newly formed apatite deposit, were dependent on the type of the used modifier, and the artificial fluid used in in vitro studies. |
| first_indexed | 2024-03-10T12:46:13Z |
| format | Article |
| id | doaj.art-2176e6fdaba546ea9d643de8c66c47bb |
| institution | Directory Open Access Journal |
| issn | 2079-4983 |
| language | English |
| last_indexed | 2024-03-10T12:46:13Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Functional Biomaterials |
| spelling | doaj.art-2176e6fdaba546ea9d643de8c66c47bb2023-11-21T13:27:40ZengMDPI AGJournal of Functional Biomaterials2079-49832021-03-011222110.3390/jfb12020021Synthesis and Characterization of Polymer-Based Coatings Modified with Bioactive Ceramic and Bovine Serum AlbuminWioletta Florkiewicz0Dagmara Słota1Angelika Placek2Klaudia Pluta3Bożena Tyliszczak4Timothy E. L. Douglas5Agnieszka Sobczak-Kupiec6Institute of Materials Science, Faculty of Materials Science and Physics, Cracow University of Technology, 37 Jana Pawła II Av., 31-864 Krakow, PolandInstitute of Materials Science, Faculty of Materials Science and Physics, Cracow University of Technology, 37 Jana Pawła II Av., 31-864 Krakow, PolandInstitute of Inorganic Chemistry and Technology, Cracow University of Technology, 24 Warszawska St., 31-155 Krakow, PolandInstitute of Inorganic Chemistry and Technology, Cracow University of Technology, 24 Warszawska St., 31-155 Krakow, PolandInstitute of Materials Science, Faculty of Materials Science and Physics, Cracow University of Technology, 37 Jana Pawła II Av., 31-864 Krakow, PolandEngineering Department, Lancaster University, Gillow Av., Lancaster LA1 4YW, UKInstitute of Materials Science, Faculty of Materials Science and Physics, Cracow University of Technology, 37 Jana Pawła II Av., 31-864 Krakow, PolandThis study involves the synthesis of hydroxyapatite and describes the preparation and characterization of polymer coatings based on poly(ethylene glycol) diacrylate and poly(ethylene glycol) and modified with bovine serum albumin and hydroxyapatite. Hydroxyapatite was obtained by wet chemical synthesis and characterized by X-ray diffraction and FTIR spectroscopy, and its Ca/P molar ratio was determined (1.69 ± 0.08). The ceramic and bovine serum albumin were used in the preparation of composite materials with the polymeric matrix. The chemical composition of coatings was characterized with FTIR spectroscopy, and their morphology was recorded with SEM imaging. Moreover, the measurements of surface roughness parameters and stereometric research were performed. The prepared coatings were subjected to in vitro studies in simulated body fluid and artificial saliva. Changes in chemical composition and morphology after immersion were examined with FTIR spectroscopy and SEM imaging. Based on the conducted research, it can be stated that applied modifiers promote the biomineralization process. The roughness analysis confirmed prepared materials were characterized by the micrometer-scale topography. The materials morphology and roughness, and the morphology of the newly formed apatite deposit, were dependent on the type of the used modifier, and the artificial fluid used in in vitro studies.https://www.mdpi.com/2079-4983/12/2/21hydroxyapatitebovine serum albuminpolyethylene glycolcomposite coatingsbiomineralization |
| spellingShingle | Wioletta Florkiewicz Dagmara Słota Angelika Placek Klaudia Pluta Bożena Tyliszczak Timothy E. L. Douglas Agnieszka Sobczak-Kupiec Synthesis and Characterization of Polymer-Based Coatings Modified with Bioactive Ceramic and Bovine Serum Albumin Journal of Functional Biomaterials hydroxyapatite bovine serum albumin polyethylene glycol composite coatings biomineralization |
| title | Synthesis and Characterization of Polymer-Based Coatings Modified with Bioactive Ceramic and Bovine Serum Albumin |
| title_full | Synthesis and Characterization of Polymer-Based Coatings Modified with Bioactive Ceramic and Bovine Serum Albumin |
| title_fullStr | Synthesis and Characterization of Polymer-Based Coatings Modified with Bioactive Ceramic and Bovine Serum Albumin |
| title_full_unstemmed | Synthesis and Characterization of Polymer-Based Coatings Modified with Bioactive Ceramic and Bovine Serum Albumin |
| title_short | Synthesis and Characterization of Polymer-Based Coatings Modified with Bioactive Ceramic and Bovine Serum Albumin |
| title_sort | synthesis and characterization of polymer based coatings modified with bioactive ceramic and bovine serum albumin |
| topic | hydroxyapatite bovine serum albumin polyethylene glycol composite coatings biomineralization |
| url | https://www.mdpi.com/2079-4983/12/2/21 |
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