P1 of strawberry vein banding virus, a multilocalized protein, functions as a movement protein and interacts with the coat protein
Although the complete nucleotide sequence of strawberry vein banding virus (SVBV) has been determined and bioinformatic analysis has revealed that the SVBV genome could encode seven proteins, the precise function of each protein is unclear. This study provided evidence that the P1 protein of SVBV (S...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-04-01
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| Series: | Journal of Integrative Agriculture |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311921637115 |
| _version_ | 1818157364152369152 |
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| author | Peng-huan RUI Zhan-qi WANG Wen-shu SHAN Wei-wei XIA Xiu-hong ZHOU Lian-lian YANG Lei JIANG Tong JIANG |
| author_facet | Peng-huan RUI Zhan-qi WANG Wen-shu SHAN Wei-wei XIA Xiu-hong ZHOU Lian-lian YANG Lei JIANG Tong JIANG |
| author_sort | Peng-huan RUI |
| collection | DOAJ |
| description | Although the complete nucleotide sequence of strawberry vein banding virus (SVBV) has been determined and bioinformatic analysis has revealed that the SVBV genome could encode seven proteins, the precise function of each protein is unclear. This study provided evidence that the P1 protein of SVBV (SVBV-P1) possesses the following features. Bioinformatic and subcellular localization analyses showed that SVBV-P1 is localized in the cytoplasm and cell walls of epidermal cells in Nicotiana benthamiana, and it forms inclusion bodies associated with microtubules and the endoplasmic reticulum. Dilution experiments demonstrated that SVBV-P1 could move from the original agro-infiltrated cells to adjacent cells in N. benthamiana leaves. Further trans-complementation experiments demonstrated that SVBV-P1 could facilitate the intercellular movement of a movement-deficient potato virus X mutant in N. benthamiana leaves. Finally, yeast two-hybrid and bimolecular fluorescence complementation assays revealed that SVBV-P1 could interact with the SVBV coat protein, which is a major component of Caulimovirus virions. Results of the electrophoretic mobility shift assay indicated that SVBV-P1 lacks DNA-binding capability. In summary, the results suggest that SVBV-P1 is probably a movement protein of SVBV, providing new insights into the function of movement proteins of the Caulimovirus genus. |
| first_indexed | 2024-12-11T15:13:01Z |
| format | Article |
| id | doaj.art-21776f030d6e437f9d745a78fcdb17d2 |
| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-12-11T15:13:01Z |
| publishDate | 2022-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj.art-21776f030d6e437f9d745a78fcdb17d22022-12-22T01:00:41ZengElsevierJournal of Integrative Agriculture2095-31192022-04-0121410711083P1 of strawberry vein banding virus, a multilocalized protein, functions as a movement protein and interacts with the coat proteinPeng-huan RUI0Zhan-qi WANG1Wen-shu SHAN2Wei-wei XIA3Xiu-hong ZHOU4Lian-lian YANG5Lei JIANG6Tong JIANG7School of Plant Protection, Anhui Agricultural University, Hefei 230036, P.R.ChinaKey Laboratory of Vector Biology and Pathogen Control of Zhejiang Province, College of Life Sciences, Huzhou University, Huzhou 313000, P.R.ChinaSchool of Plant Protection, Anhui Agricultural University, Hefei 230036, P.R.ChinaSchool of Plant Protection, Anhui Agricultural University, Hefei 230036, P.R.ChinaBiotechnology Center, Anhui Agricultural University, Hefei 230036, P.R.ChinaKey Laboratory of Vector Biology and Pathogen Control of Zhejiang Province, College of Life Sciences, Huzhou University, Huzhou 313000, P.R.ChinaSchool of Plant Protection, Anhui Agricultural University, Hefei 230036, P.R.China; Anhui Province Key Laboratory of Integrated Pest Management on Crops, Hefei 230036, P.R.China; Key Laboratory of Biology and Sustainable Management of Plant Diseases and Pests of Anhui Higher Education Institutes, Anhui Agricultural University, Hefei 230036, P.R.China; Correspondence JIANG Lei, Tel: +86-551-65786312School of Plant Protection, Anhui Agricultural University, Hefei 230036, P.R.China; Anhui Province Key Laboratory of Integrated Pest Management on Crops, Hefei 230036, P.R.China; Key Laboratory of Biology and Sustainable Management of Plant Diseases and Pests of Anhui Higher Education Institutes, Anhui Agricultural University, Hefei 230036, P.R.China; Correspondence JIANG Tong, Tel: +86-551-65786312Although the complete nucleotide sequence of strawberry vein banding virus (SVBV) has been determined and bioinformatic analysis has revealed that the SVBV genome could encode seven proteins, the precise function of each protein is unclear. This study provided evidence that the P1 protein of SVBV (SVBV-P1) possesses the following features. Bioinformatic and subcellular localization analyses showed that SVBV-P1 is localized in the cytoplasm and cell walls of epidermal cells in Nicotiana benthamiana, and it forms inclusion bodies associated with microtubules and the endoplasmic reticulum. Dilution experiments demonstrated that SVBV-P1 could move from the original agro-infiltrated cells to adjacent cells in N. benthamiana leaves. Further trans-complementation experiments demonstrated that SVBV-P1 could facilitate the intercellular movement of a movement-deficient potato virus X mutant in N. benthamiana leaves. Finally, yeast two-hybrid and bimolecular fluorescence complementation assays revealed that SVBV-P1 could interact with the SVBV coat protein, which is a major component of Caulimovirus virions. Results of the electrophoretic mobility shift assay indicated that SVBV-P1 lacks DNA-binding capability. In summary, the results suggest that SVBV-P1 is probably a movement protein of SVBV, providing new insights into the function of movement proteins of the Caulimovirus genus.http://www.sciencedirect.com/science/article/pii/S2095311921637115strawberry vein banding virusP1 proteinmovement proteincoat proteinvirus movement |
| spellingShingle | Peng-huan RUI Zhan-qi WANG Wen-shu SHAN Wei-wei XIA Xiu-hong ZHOU Lian-lian YANG Lei JIANG Tong JIANG P1 of strawberry vein banding virus, a multilocalized protein, functions as a movement protein and interacts with the coat protein Journal of Integrative Agriculture strawberry vein banding virus P1 protein movement protein coat protein virus movement |
| title | P1 of strawberry vein banding virus, a multilocalized protein, functions as a movement protein and interacts with the coat protein |
| title_full | P1 of strawberry vein banding virus, a multilocalized protein, functions as a movement protein and interacts with the coat protein |
| title_fullStr | P1 of strawberry vein banding virus, a multilocalized protein, functions as a movement protein and interacts with the coat protein |
| title_full_unstemmed | P1 of strawberry vein banding virus, a multilocalized protein, functions as a movement protein and interacts with the coat protein |
| title_short | P1 of strawberry vein banding virus, a multilocalized protein, functions as a movement protein and interacts with the coat protein |
| title_sort | p1 of strawberry vein banding virus a multilocalized protein functions as a movement protein and interacts with the coat protein |
| topic | strawberry vein banding virus P1 protein movement protein coat protein virus movement |
| url | http://www.sciencedirect.com/science/article/pii/S2095311921637115 |
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