Amino acid biosynthetic and antioxidant activity of Brevibacterium sp. InaCC B46 using glucose substrate produced by saccharification of oil palm trunk biomass
Brevibacterium sp. is commercially essential due to its production of amino acids, particularly glutamic acid and lysine. This work investigates the effect of Brevibacterium sp. concentration on L-amino acid synthesis from glucose hydrolysed from oil palm trunk biomass as a substrate. The study empl...
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| Format: | Article |
| Language: | English |
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EDP Sciences
2024-01-01
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| Series: | BIO Web of Conferences |
| Online Access: | https://www.bio-conferences.org/articles/bioconf/pdf/2024/18/bioconf_icafes2024_02003.pdf |
| _version_ | 1797221539263807488 |
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| author | Riftyan Emma Yusmarini Yusmarini Fitriani Shanti Pato Usman Rossi Evy Nadapdap Novianti Monica |
| author_facet | Riftyan Emma Yusmarini Yusmarini Fitriani Shanti Pato Usman Rossi Evy Nadapdap Novianti Monica |
| author_sort | Riftyan Emma |
| collection | DOAJ |
| description | Brevibacterium sp. is commercially essential due to its production of amino acids, particularly glutamic acid and lysine. This work investigates the effect of Brevibacterium sp. concentration on L-amino acid synthesis from glucose hydrolysed from oil palm trunk biomass as a substrate. The study employed a two-factorial, completely randomised experimental design. Factor A consisted of concentrations of Brevibacterium sp. (1%, 3%, and 5%), while factor B was incubation duration (24 and 48 hours). The study measured the concentrations of four amino acids (alanine, glycine, tyrosine, and glutamic acid) using the colourimetric ninhydrin reaction. Additionally, the viability and antioxidant activity of Brevibacterium sp. during incubation time was measured with spectrophotometry (UV-VIS). The results indicate no significant interaction between factors (P>0.05) but a significant effect (P<0.05) depending on the concentration and incubation time of Brevibacterium sp. InaCCB46 for all parameters. The study found that the biosynthesis of amino acids was most efficient with a 5% concentration of bacteria during 24-hour incubation, resulting in the highest output of alanine, glycine, tyrosine, and glutamic acid at 6.46, 4.58, 6.16, and 6.67 µg/mL, respectively. However, it should be noted that bacterial viability was higher after 24 hours of incubation, ranging from 144.18–216.99% and had strong antioxidant activity at 22.43 ppm. |
| first_indexed | 2024-04-24T13:07:02Z |
| format | Article |
| id | doaj.art-217a537d681b4c389829caf570790aa5 |
| institution | Directory Open Access Journal |
| issn | 2117-4458 |
| language | English |
| last_indexed | 2024-04-24T13:07:02Z |
| publishDate | 2024-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | BIO Web of Conferences |
| spelling | doaj.art-217a537d681b4c389829caf570790aa52024-04-05T07:29:40ZengEDP SciencesBIO Web of Conferences2117-44582024-01-01990200310.1051/bioconf/20249902003bioconf_icafes2024_02003Amino acid biosynthetic and antioxidant activity of Brevibacterium sp. InaCC B46 using glucose substrate produced by saccharification of oil palm trunk biomassRiftyan Emma0Yusmarini Yusmarini1Fitriani Shanti2Pato Usman3Rossi Evy4Nadapdap Novianti Monica5Faculty of Agricultural, Universitas RiauFaculty of Agricultural, Universitas RiauFaculty of Agricultural, Universitas RiauFaculty of Agricultural, Universitas RiauFaculty of Agricultural, Universitas RiauFaculty of Agricultural, Universitas RiauBrevibacterium sp. is commercially essential due to its production of amino acids, particularly glutamic acid and lysine. This work investigates the effect of Brevibacterium sp. concentration on L-amino acid synthesis from glucose hydrolysed from oil palm trunk biomass as a substrate. The study employed a two-factorial, completely randomised experimental design. Factor A consisted of concentrations of Brevibacterium sp. (1%, 3%, and 5%), while factor B was incubation duration (24 and 48 hours). The study measured the concentrations of four amino acids (alanine, glycine, tyrosine, and glutamic acid) using the colourimetric ninhydrin reaction. Additionally, the viability and antioxidant activity of Brevibacterium sp. during incubation time was measured with spectrophotometry (UV-VIS). The results indicate no significant interaction between factors (P>0.05) but a significant effect (P<0.05) depending on the concentration and incubation time of Brevibacterium sp. InaCCB46 for all parameters. The study found that the biosynthesis of amino acids was most efficient with a 5% concentration of bacteria during 24-hour incubation, resulting in the highest output of alanine, glycine, tyrosine, and glutamic acid at 6.46, 4.58, 6.16, and 6.67 µg/mL, respectively. However, it should be noted that bacterial viability was higher after 24 hours of incubation, ranging from 144.18–216.99% and had strong antioxidant activity at 22.43 ppm.https://www.bio-conferences.org/articles/bioconf/pdf/2024/18/bioconf_icafes2024_02003.pdf |
| spellingShingle | Riftyan Emma Yusmarini Yusmarini Fitriani Shanti Pato Usman Rossi Evy Nadapdap Novianti Monica Amino acid biosynthetic and antioxidant activity of Brevibacterium sp. InaCC B46 using glucose substrate produced by saccharification of oil palm trunk biomass BIO Web of Conferences |
| title | Amino acid biosynthetic and antioxidant activity of Brevibacterium sp. InaCC B46 using glucose substrate produced by saccharification of oil palm trunk biomass |
| title_full | Amino acid biosynthetic and antioxidant activity of Brevibacterium sp. InaCC B46 using glucose substrate produced by saccharification of oil palm trunk biomass |
| title_fullStr | Amino acid biosynthetic and antioxidant activity of Brevibacterium sp. InaCC B46 using glucose substrate produced by saccharification of oil palm trunk biomass |
| title_full_unstemmed | Amino acid biosynthetic and antioxidant activity of Brevibacterium sp. InaCC B46 using glucose substrate produced by saccharification of oil palm trunk biomass |
| title_short | Amino acid biosynthetic and antioxidant activity of Brevibacterium sp. InaCC B46 using glucose substrate produced by saccharification of oil palm trunk biomass |
| title_sort | amino acid biosynthetic and antioxidant activity of brevibacterium sp inacc b46 using glucose substrate produced by saccharification of oil palm trunk biomass |
| url | https://www.bio-conferences.org/articles/bioconf/pdf/2024/18/bioconf_icafes2024_02003.pdf |
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