Sialic Acid Binding Properties of Soluble Coronavirus Spike (S1) Proteins: Differences between Infectious Bronchitis Virus and Transmissible Gastroenteritis Virus
The spike proteins of a number of coronaviruses are able to bind to sialic acids present on the cell surface. The importance of this sialic acid binding ability during infection is, however, quite different. We compared the spike protein of transmissible gastroenteritis virus (TGEV) and the spike pr...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2013-07-01
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| Series: | Viruses |
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| Online Access: | http://www.mdpi.com/1999-4915/5/8/1924 |
| _version_ | 1811253661224927232 |
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| author | Christine Winter Georg Herrler Ann-Kathrin Mork Martina Hesse Katarina Shahwan |
| author_facet | Christine Winter Georg Herrler Ann-Kathrin Mork Martina Hesse Katarina Shahwan |
| author_sort | Christine Winter |
| collection | DOAJ |
| description | The spike proteins of a number of coronaviruses are able to bind to sialic acids present on the cell surface. The importance of this sialic acid binding ability during infection is, however, quite different. We compared the spike protein of transmissible gastroenteritis virus (TGEV) and the spike protein of infectious bronchitis virus (IBV). Whereas sialic acid is the only receptor determinant known so far for IBV, TGEV requires interaction with its receptor aminopeptidase N to initiate infection of cells. Binding tests with soluble spike proteins carrying an IgG Fc-tag revealed pronounced differences between these two viral proteins. Binding of the IBV spike protein to host cells was in all experiments sialic acid dependent, whereas the soluble TGEV spike showed binding to APN but had no detectable sialic acid binding activity. Our results underline the different ways in which binding to sialoglycoconjugates is mediated by coronavirus spike proteins. |
| first_indexed | 2024-04-12T16:54:21Z |
| format | Article |
| id | doaj.art-217d859e1d40414c910f1e48f36ce107 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-04-12T16:54:21Z |
| publishDate | 2013-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-217d859e1d40414c910f1e48f36ce1072022-12-22T03:24:17ZengMDPI AGViruses1999-49152013-07-01581924193310.3390/v5081924Sialic Acid Binding Properties of Soluble Coronavirus Spike (S1) Proteins: Differences between Infectious Bronchitis Virus and Transmissible Gastroenteritis VirusChristine WinterGeorg HerrlerAnn-Kathrin MorkMartina HesseKatarina ShahwanThe spike proteins of a number of coronaviruses are able to bind to sialic acids present on the cell surface. The importance of this sialic acid binding ability during infection is, however, quite different. We compared the spike protein of transmissible gastroenteritis virus (TGEV) and the spike protein of infectious bronchitis virus (IBV). Whereas sialic acid is the only receptor determinant known so far for IBV, TGEV requires interaction with its receptor aminopeptidase N to initiate infection of cells. Binding tests with soluble spike proteins carrying an IgG Fc-tag revealed pronounced differences between these two viral proteins. Binding of the IBV spike protein to host cells was in all experiments sialic acid dependent, whereas the soluble TGEV spike showed binding to APN but had no detectable sialic acid binding activity. Our results underline the different ways in which binding to sialoglycoconjugates is mediated by coronavirus spike proteins.http://www.mdpi.com/1999-4915/5/8/1924Coronavirusspike proteinsialic acid receptorTGEVIBV |
| spellingShingle | Christine Winter Georg Herrler Ann-Kathrin Mork Martina Hesse Katarina Shahwan Sialic Acid Binding Properties of Soluble Coronavirus Spike (S1) Proteins: Differences between Infectious Bronchitis Virus and Transmissible Gastroenteritis Virus Viruses Coronavirus spike protein sialic acid receptor TGEV IBV |
| title | Sialic Acid Binding Properties of Soluble Coronavirus Spike (S1) Proteins: Differences between Infectious Bronchitis Virus and Transmissible Gastroenteritis Virus |
| title_full | Sialic Acid Binding Properties of Soluble Coronavirus Spike (S1) Proteins: Differences between Infectious Bronchitis Virus and Transmissible Gastroenteritis Virus |
| title_fullStr | Sialic Acid Binding Properties of Soluble Coronavirus Spike (S1) Proteins: Differences between Infectious Bronchitis Virus and Transmissible Gastroenteritis Virus |
| title_full_unstemmed | Sialic Acid Binding Properties of Soluble Coronavirus Spike (S1) Proteins: Differences between Infectious Bronchitis Virus and Transmissible Gastroenteritis Virus |
| title_short | Sialic Acid Binding Properties of Soluble Coronavirus Spike (S1) Proteins: Differences between Infectious Bronchitis Virus and Transmissible Gastroenteritis Virus |
| title_sort | sialic acid binding properties of soluble coronavirus spike s1 proteins differences between infectious bronchitis virus and transmissible gastroenteritis virus |
| topic | Coronavirus spike protein sialic acid receptor TGEV IBV |
| url | http://www.mdpi.com/1999-4915/5/8/1924 |
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