Cellular encoding of Cy dyes for single-molecule imaging

Cellular encoding of Cy dyes for single-molecule imaging

A general method is described for the site-specific genetic encoding of cyanine dyes as non-canonical amino acids (Cy-ncAAs) into proteins. The approach relies on an improved technique for nonsense suppression with in vitro misacylated orthogonal tRNA. The data show that Cy-ncAAs (based on Cy3 and C...

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Bibliographic Details
Main Authors: Lilia Leisle, Rahul Chadda, John D Lueck, Daniel T Infield, Jason D Galpin, Venkatramanan Krishnamani, Janice L Robertson, Christopher A Ahern
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2016-12-01
Series:eLife
Subjects:
single molecule imaging
genetic code expansion
TIRF
membrane proteins
cell-free protein synthesis
orthogonal tRNA
Online Access:https://elifesciences.org/articles/19088

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