β-arrestin2 Mediates the Arginine Vasopressin-Induced Expression of IL-1β in Murine Hearts
Background: Circulating levels of arginine vasopressin (AVP) are elevated during cardiac stress and this could be a factor in cardiac inflammation and fibrosis. Herein, we studied the effects of AVP on interleukin-1β (IL-1β) production and the role(s) of β-arrestin2-dependent signaling in murine hea...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
IMR Press
2023-01-01
|
| Series: | Frontiers in Bioscience-Landmark |
| Subjects: | |
| Online Access: | https://www.imrpress.com/journal/FBL/28/1/10.31083/j.fbl2801007 |
| _version_ | 1828047803320893440 |
|---|---|
| author | Na Yao Beibei Guo Yuhang Wang Ying Hu Xiaofang Zhu Jiaxin Cao Yi Liu Yi Qian Hua Sang Weizhong Zhu |
| author_facet | Na Yao Beibei Guo Yuhang Wang Ying Hu Xiaofang Zhu Jiaxin Cao Yi Liu Yi Qian Hua Sang Weizhong Zhu |
| author_sort | Na Yao |
| collection | DOAJ |
| description | Background: Circulating levels of arginine vasopressin (AVP) are elevated during cardiac stress and this could be a factor in cardiac inflammation and fibrosis. Herein, we studied the effects of AVP on interleukin-1β (IL-1β) production and the role(s) of β-arrestin2-dependent signaling in murine heart. Methods: The levels of IL-1β mRNA and protein in adult rat cardiofibroblasts (ARCFs) was measured using quantitative PCR and ELISA, respectively. The activity of β-arrestin2 was manipulated using either pharmacologic inhibitors or through recombinant β-arrestin2 over-expression. These experiments were conducted to determine the roles of β-arrestin2 in the regulation of AVP-induced IL-1β and NLRP3 inflammasome production. The phosphorylation and activation of NF-κB induced by AVP was measured by immunoblotting. β-arrestin2 knockout (KO) mice were used to investigate whether β-arrestin2 mediated the AVP-induced production of IL-1β and NLRP3, as well as the phosphorylation of the NF-κB p65 subunitin mouse myocardium. Prism GraphPad software(version 8.0), was used for all statistical analyses. Results: AVP induced the expression of IL-1β in a time-dependent manner in ARCFs but not in cultured adult rat cardiomyocytes (ARCMs). The inhibition of NF-κB with pyrrolidinedithiocarbamic acid (PDTC) prevented the AVP-induced phosphorylation of NF-κB and production of IL-1β and NLRP3 in ARCFs. The deletion of β-arrestin2 blocked the phosphorylation of p65 and the expression of NLRP3 and IL-1β induced by AVP in both mouse hearts and in ARCFs. Conclusions: AVP promotes IL-1β expression through β-arrestin2-mediated NF-κB signaling in murine heart. |
| first_indexed | 2024-04-10T18:47:52Z |
| format | Article |
| id | doaj.art-21811f04256f42869929788c82372182 |
| institution | Directory Open Access Journal |
| issn | 2768-6701 |
| language | English |
| last_indexed | 2024-04-10T18:47:52Z |
| publishDate | 2023-01-01 |
| publisher | IMR Press |
| record_format | Article |
| series | Frontiers in Bioscience-Landmark |
| spelling | doaj.art-21811f04256f42869929788c823721822023-02-01T09:21:18ZengIMR PressFrontiers in Bioscience-Landmark2768-67012023-01-01281710.31083/j.fbl2801007S2768-6701(22)00682-7β-arrestin2 Mediates the Arginine Vasopressin-Induced Expression of IL-1β in Murine HeartsNa Yao0Beibei Guo1Yuhang Wang2Ying Hu3Xiaofang Zhu4Jiaxin Cao5Yi Liu6Yi Qian7Hua Sang8Weizhong Zhu9Cardiovascular Laboratory, Department of Pharmacology, School of Pharmacy, Nantong University, 226001 Nantong, Jiangsu, ChinaCardiovascular Laboratory, Department of Pharmacology, School of Pharmacy, Nantong University, 226001 Nantong, Jiangsu, ChinaCardiovascular Laboratory, Department of Pharmacology, School of Pharmacy, Nantong University, 226001 Nantong, Jiangsu, ChinaCardiovascular Laboratory, Department of Pharmacology, School of Pharmacy, Nantong University, 226001 Nantong, Jiangsu, ChinaCardiovascular Laboratory, Department of Pharmacology, School of Pharmacy, Nantong University, 226001 Nantong, Jiangsu, ChinaCardiovascular Laboratory, Department of Pharmacology, School of Pharmacy, Nantong University, 226001 Nantong, Jiangsu, ChinaCardiovascular Laboratory, Department of Pharmacology, School of Pharmacy, Nantong University, 226001 Nantong, Jiangsu, ChinaCardiovascular Laboratory, Department of Pharmacology, School of Pharmacy, Nantong University, 226001 Nantong, Jiangsu, ChinaAffiliated Hospital of Nantong University, 226001 Nantong, Jiangsu, ChinaCardiovascular Laboratory, Department of Pharmacology, School of Pharmacy, Nantong University, 226001 Nantong, Jiangsu, ChinaBackground: Circulating levels of arginine vasopressin (AVP) are elevated during cardiac stress and this could be a factor in cardiac inflammation and fibrosis. Herein, we studied the effects of AVP on interleukin-1β (IL-1β) production and the role(s) of β-arrestin2-dependent signaling in murine heart. Methods: The levels of IL-1β mRNA and protein in adult rat cardiofibroblasts (ARCFs) was measured using quantitative PCR and ELISA, respectively. The activity of β-arrestin2 was manipulated using either pharmacologic inhibitors or through recombinant β-arrestin2 over-expression. These experiments were conducted to determine the roles of β-arrestin2 in the regulation of AVP-induced IL-1β and NLRP3 inflammasome production. The phosphorylation and activation of NF-κB induced by AVP was measured by immunoblotting. β-arrestin2 knockout (KO) mice were used to investigate whether β-arrestin2 mediated the AVP-induced production of IL-1β and NLRP3, as well as the phosphorylation of the NF-κB p65 subunitin mouse myocardium. Prism GraphPad software(version 8.0), was used for all statistical analyses. Results: AVP induced the expression of IL-1β in a time-dependent manner in ARCFs but not in cultured adult rat cardiomyocytes (ARCMs). The inhibition of NF-κB with pyrrolidinedithiocarbamic acid (PDTC) prevented the AVP-induced phosphorylation of NF-κB and production of IL-1β and NLRP3 in ARCFs. The deletion of β-arrestin2 blocked the phosphorylation of p65 and the expression of NLRP3 and IL-1β induced by AVP in both mouse hearts and in ARCFs. Conclusions: AVP promotes IL-1β expression through β-arrestin2-mediated NF-κB signaling in murine heart.https://www.imrpress.com/journal/FBL/28/1/10.31083/j.fbl2801007arginine vasopressinβ-arrestin 2nf-κb p65nlrp3 inflammasomeinterlukin-1βinflammationmurine |
| spellingShingle | Na Yao Beibei Guo Yuhang Wang Ying Hu Xiaofang Zhu Jiaxin Cao Yi Liu Yi Qian Hua Sang Weizhong Zhu β-arrestin2 Mediates the Arginine Vasopressin-Induced Expression of IL-1β in Murine Hearts Frontiers in Bioscience-Landmark arginine vasopressin β-arrestin 2 nf-κb p65 nlrp3 inflammasome interlukin-1β inflammation murine |
| title | β-arrestin2 Mediates the Arginine Vasopressin-Induced Expression of IL-1β in Murine Hearts |
| title_full | β-arrestin2 Mediates the Arginine Vasopressin-Induced Expression of IL-1β in Murine Hearts |
| title_fullStr | β-arrestin2 Mediates the Arginine Vasopressin-Induced Expression of IL-1β in Murine Hearts |
| title_full_unstemmed | β-arrestin2 Mediates the Arginine Vasopressin-Induced Expression of IL-1β in Murine Hearts |
| title_short | β-arrestin2 Mediates the Arginine Vasopressin-Induced Expression of IL-1β in Murine Hearts |
| title_sort | β arrestin2 mediates the arginine vasopressin induced expression of il 1β in murine hearts |
| topic | arginine vasopressin β-arrestin 2 nf-κb p65 nlrp3 inflammasome interlukin-1β inflammation murine |
| url | https://www.imrpress.com/journal/FBL/28/1/10.31083/j.fbl2801007 |
| work_keys_str_mv | AT nayao barrestin2mediatestheargininevasopressininducedexpressionofil1binmurinehearts AT beibeiguo barrestin2mediatestheargininevasopressininducedexpressionofil1binmurinehearts AT yuhangwang barrestin2mediatestheargininevasopressininducedexpressionofil1binmurinehearts AT yinghu barrestin2mediatestheargininevasopressininducedexpressionofil1binmurinehearts AT xiaofangzhu barrestin2mediatestheargininevasopressininducedexpressionofil1binmurinehearts AT jiaxincao barrestin2mediatestheargininevasopressininducedexpressionofil1binmurinehearts AT yiliu barrestin2mediatestheargininevasopressininducedexpressionofil1binmurinehearts AT yiqian barrestin2mediatestheargininevasopressininducedexpressionofil1binmurinehearts AT huasang barrestin2mediatestheargininevasopressininducedexpressionofil1binmurinehearts AT weizhongzhu barrestin2mediatestheargininevasopressininducedexpressionofil1binmurinehearts |