Huangqi decoction ameliorates kidney injury in db/db mice by regulating the BMP/Smad signaling pathway
Abstract Purpose This study aims to investigate the mechanism underlying the beneficial effects of Huangqi decoction (HQD) on Diabetic kidney disease (DKD) in diabetic db/db mice. Methods Eight-week-old male diabetic db/db mice were randomly divided into four groups: Model (1% CMC), HQD-L (0.12 g/kg...
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| Format: | Article |
| Language: | English |
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BMC
2023-06-01
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| Series: | BMC Complementary Medicine and Therapies |
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| Online Access: | https://doi.org/10.1186/s12906-023-04029-1 |
| _version_ | 1797789898252484608 |
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| author | Ying Chen Rong Rui Li Wang Hao Wang Bingbing Zhu Aili Cao |
| author_facet | Ying Chen Rong Rui Li Wang Hao Wang Bingbing Zhu Aili Cao |
| author_sort | Ying Chen |
| collection | DOAJ |
| description | Abstract Purpose This study aims to investigate the mechanism underlying the beneficial effects of Huangqi decoction (HQD) on Diabetic kidney disease (DKD) in diabetic db/db mice. Methods Eight-week-old male diabetic db/db mice were randomly divided into four groups: Model (1% CMC), HQD-L (0.12 g/kg), HQD-M (0.36 g/kg), and HQD-H (1.08 g/kg) groups. Non-diabetic db/m mice were served as the control group. These mice received HQD treatment for 8 weeks. After treatment, the kidney function, histopathology, micro-assay, and protein expression levels were assessed. Results HQD treatment improved the albumin/creatine ratio (ACR) and 24 h urinary albumin excretion, prevented the pathological phenotypes of increased glomerular volume, widened mesangial areas, the of mesangial matrix proliferation, foot process effacement, decreased nephrin expression and reduced number of podocytes. Expression profiling analysis revealed global transcriptional changes that predicted related functions, diseases and pathways. HQD treatment activated protein expressions of BMP2, BMP7, BMPR2, and active-Rap1, while inhibiting Smad1 and phospho-ERK. In addition, HQD was associated with improvements in lipid deposition in the kidneys of db/db mice. Conclusion HQD ameliorated the progression of DKD in db/db mice by regulating BMP transcription and downstream targets, inhibiting the phosphorylation of ERK and the expression of Smad1, promoting Rap1 binding to GTP, and regulating the lipid metabolism. These findings provide a potential therapeutic approach for treating DKD. |
| first_indexed | 2024-03-13T01:57:08Z |
| format | Article |
| id | doaj.art-21813464e5894c4aaacc9c8d2dbe8832 |
| institution | Directory Open Access Journal |
| issn | 2662-7671 |
| language | English |
| last_indexed | 2024-03-13T01:57:08Z |
| publishDate | 2023-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary Medicine and Therapies |
| spelling | doaj.art-21813464e5894c4aaacc9c8d2dbe88322023-07-02T11:07:05ZengBMCBMC Complementary Medicine and Therapies2662-76712023-06-0123111310.1186/s12906-023-04029-1Huangqi decoction ameliorates kidney injury in db/db mice by regulating the BMP/Smad signaling pathwayYing Chen0Rong Rui1Li Wang2Hao Wang3Bingbing Zhu4Aili Cao5Department of Nephrology, Laboratory of Renal Disease, Putuo Hospital, Shanghai University of Traditional Chinese MedicineDepartment of Nephrology, Laboratory of Renal Disease, Putuo Hospital, Shanghai University of Traditional Chinese MedicineDepartment of Nephrology, Laboratory of Renal Disease, Putuo Hospital, Shanghai University of Traditional Chinese MedicineDepartment of Nephrology, Laboratory of Renal Disease, Putuo Hospital, Shanghai University of Traditional Chinese MedicineDepartment of Nephrology, Laboratory of Renal Disease, Putuo Hospital, Shanghai University of Traditional Chinese MedicineDepartment of Nephrology, Shanghai Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University School of MedicineAbstract Purpose This study aims to investigate the mechanism underlying the beneficial effects of Huangqi decoction (HQD) on Diabetic kidney disease (DKD) in diabetic db/db mice. Methods Eight-week-old male diabetic db/db mice were randomly divided into four groups: Model (1% CMC), HQD-L (0.12 g/kg), HQD-M (0.36 g/kg), and HQD-H (1.08 g/kg) groups. Non-diabetic db/m mice were served as the control group. These mice received HQD treatment for 8 weeks. After treatment, the kidney function, histopathology, micro-assay, and protein expression levels were assessed. Results HQD treatment improved the albumin/creatine ratio (ACR) and 24 h urinary albumin excretion, prevented the pathological phenotypes of increased glomerular volume, widened mesangial areas, the of mesangial matrix proliferation, foot process effacement, decreased nephrin expression and reduced number of podocytes. Expression profiling analysis revealed global transcriptional changes that predicted related functions, diseases and pathways. HQD treatment activated protein expressions of BMP2, BMP7, BMPR2, and active-Rap1, while inhibiting Smad1 and phospho-ERK. In addition, HQD was associated with improvements in lipid deposition in the kidneys of db/db mice. Conclusion HQD ameliorated the progression of DKD in db/db mice by regulating BMP transcription and downstream targets, inhibiting the phosphorylation of ERK and the expression of Smad1, promoting Rap1 binding to GTP, and regulating the lipid metabolism. These findings provide a potential therapeutic approach for treating DKD.https://doi.org/10.1186/s12906-023-04029-1Huangqi decoction (HQD)Diabetic kidney disease (DKD)Lipid metabolismDb/db mice |
| spellingShingle | Ying Chen Rong Rui Li Wang Hao Wang Bingbing Zhu Aili Cao Huangqi decoction ameliorates kidney injury in db/db mice by regulating the BMP/Smad signaling pathway BMC Complementary Medicine and Therapies Huangqi decoction (HQD) Diabetic kidney disease (DKD) Lipid metabolism Db/db mice |
| title | Huangqi decoction ameliorates kidney injury in db/db mice by regulating the BMP/Smad signaling pathway |
| title_full | Huangqi decoction ameliorates kidney injury in db/db mice by regulating the BMP/Smad signaling pathway |
| title_fullStr | Huangqi decoction ameliorates kidney injury in db/db mice by regulating the BMP/Smad signaling pathway |
| title_full_unstemmed | Huangqi decoction ameliorates kidney injury in db/db mice by regulating the BMP/Smad signaling pathway |
| title_short | Huangqi decoction ameliorates kidney injury in db/db mice by regulating the BMP/Smad signaling pathway |
| title_sort | huangqi decoction ameliorates kidney injury in db db mice by regulating the bmp smad signaling pathway |
| topic | Huangqi decoction (HQD) Diabetic kidney disease (DKD) Lipid metabolism Db/db mice |
| url | https://doi.org/10.1186/s12906-023-04029-1 |
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