Genomic Association between SNP Markers and Diseases in the “Curraleiro Pé-Duro” Cattle
Susceptibility to diseases is inherited and can be transmitted between populations. Single-nucleotide polymorphism (SNPs) in genes related to immune response is associated with diseases in cattle. This study investigated SNPs in the genomic region of cytokines in 702 samples of Curraleiro Pé-Duro ca...
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| Format: | Article |
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MDPI AG
2021-05-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/12/6/806 |
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| author | Thais Miranda Silva Freitas Juliana Moraes Dias Luanna Kim Pires Guimarães Sáudio Vieira Peixoto Rayanne Henrique Santana da Silva Kareem Rady Badr Maria Ivete Moura Adriana Santana do Carmo Vincenzo Landi Maria Clorinda Soares Fioravanti |
| author_facet | Thais Miranda Silva Freitas Juliana Moraes Dias Luanna Kim Pires Guimarães Sáudio Vieira Peixoto Rayanne Henrique Santana da Silva Kareem Rady Badr Maria Ivete Moura Adriana Santana do Carmo Vincenzo Landi Maria Clorinda Soares Fioravanti |
| author_sort | Thais Miranda Silva Freitas |
| collection | DOAJ |
| description | Susceptibility to diseases is inherited and can be transmitted between populations. Single-nucleotide polymorphism (SNPs) in genes related to immune response is associated with diseases in cattle. This study investigated SNPs in the genomic region of cytokines in 702 samples of Curraleiro Pé-Duro cattle and associated them with the occurrence of antibodies in brucellosis, leptospirosis, neosporosis, leukosis, infectious bovine rhinotracheitis (IBR), and bovine viral diarrhea (BVD) tests. DNA samples were evaluated by the kompetitive allele-specific polymerase chain reaction (KASP) method to identify polymorphisms. The gametic phase and SNP haplotypes were determined with the help of PHASE 2.1.1 software. Haplotypes were associated with serological results against <i>Brucella abortus</i>, <i>Leptospira</i> sp., <i>Neospora caninum</i>, leukosis, infectious rhinotracheitis, and BVD using univariate analysis followed by logistic regression. Haplotype 2 of <i>TLR2</i> was present in 70% of the animals that tested positive for <i>N. caninum</i> infection. Haplotypes of <i>TLR10</i> and <i>TLR6</i> and <i>IL10RA</i> were more common in seronegative animals. Haplotypes related to the gene <i>IL10RA</i> were associated with animals negative to all infections. Curraleiro Pé-Duro cattle presented polymorphisms related to resistance to bacterial, viral, and <i>N. caninum</i> infections. |
| first_indexed | 2024-03-10T11:04:08Z |
| format | Article |
| id | doaj.art-21883e019f1f448eb18f4229e2151612 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-10T11:04:08Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-21883e019f1f448eb18f4229e21516122023-11-21T21:17:18ZengMDPI AGGenes2073-44252021-05-0112680610.3390/genes12060806Genomic Association between SNP Markers and Diseases in the “Curraleiro Pé-Duro” CattleThais Miranda Silva Freitas0Juliana Moraes Dias1Luanna Kim Pires Guimarães2Sáudio Vieira Peixoto3Rayanne Henrique Santana da Silva4Kareem Rady Badr5Maria Ivete Moura6Adriana Santana do Carmo7Vincenzo Landi8Maria Clorinda Soares Fioravanti9School of Veterinary and Animal Science, Federal University of Goiás, Campus Samambaia, Goiânia, Goiás 74690-900, BrazilSchool of Veterinary and Animal Science, Federal University of Goiás, Campus Samambaia, Goiânia, Goiás 74690-900, BrazilSchool of Veterinary and Animal Science, Federal University of Goiás, Campus Samambaia, Goiânia, Goiás 74690-900, BrazilSchool of Veterinary and Animal Science, Federal University of Goiás, Campus Samambaia, Goiânia, Goiás 74690-900, BrazilSchool of Veterinary and Animal Science, Federal University of Goiás, Campus Samambaia, Goiânia, Goiás 74690-900, BrazilEnvironmental Virology Laboratory, Water Pollution Research Department, National Research Centre, Dokki, Giza 12622, EgyptCourse in Veterinary Medicine, Pontifical Catholic University of Goiás, Av. Engler, Jardim Mariliza, Goiânia, Goiás 74885-460, BrazilSchool of Veterinary and Animal Science, Federal University of Goiás, Campus Samambaia, Goiânia, Goiás 74690-900, BrazilDepartment of Veterinary Medicine, University of Bari Aldo Moro, Str. Prov. per Casamassima, Km 3, 70010 Valenzano, BA, ItalySchool of Veterinary and Animal Science, Federal University of Goiás, Campus Samambaia, Goiânia, Goiás 74690-900, BrazilSusceptibility to diseases is inherited and can be transmitted between populations. Single-nucleotide polymorphism (SNPs) in genes related to immune response is associated with diseases in cattle. This study investigated SNPs in the genomic region of cytokines in 702 samples of Curraleiro Pé-Duro cattle and associated them with the occurrence of antibodies in brucellosis, leptospirosis, neosporosis, leukosis, infectious bovine rhinotracheitis (IBR), and bovine viral diarrhea (BVD) tests. DNA samples were evaluated by the kompetitive allele-specific polymerase chain reaction (KASP) method to identify polymorphisms. The gametic phase and SNP haplotypes were determined with the help of PHASE 2.1.1 software. Haplotypes were associated with serological results against <i>Brucella abortus</i>, <i>Leptospira</i> sp., <i>Neospora caninum</i>, leukosis, infectious rhinotracheitis, and BVD using univariate analysis followed by logistic regression. Haplotype 2 of <i>TLR2</i> was present in 70% of the animals that tested positive for <i>N. caninum</i> infection. Haplotypes of <i>TLR10</i> and <i>TLR6</i> and <i>IL10RA</i> were more common in seronegative animals. Haplotypes related to the gene <i>IL10RA</i> were associated with animals negative to all infections. Curraleiro Pé-Duro cattle presented polymorphisms related to resistance to bacterial, viral, and <i>N. caninum</i> infections.https://www.mdpi.com/2073-4425/12/6/806local breedpolymorphismdisease susceptibility |
| spellingShingle | Thais Miranda Silva Freitas Juliana Moraes Dias Luanna Kim Pires Guimarães Sáudio Vieira Peixoto Rayanne Henrique Santana da Silva Kareem Rady Badr Maria Ivete Moura Adriana Santana do Carmo Vincenzo Landi Maria Clorinda Soares Fioravanti Genomic Association between SNP Markers and Diseases in the “Curraleiro Pé-Duro” Cattle Genes local breed polymorphism disease susceptibility |
| title | Genomic Association between SNP Markers and Diseases in the “Curraleiro Pé-Duro” Cattle |
| title_full | Genomic Association between SNP Markers and Diseases in the “Curraleiro Pé-Duro” Cattle |
| title_fullStr | Genomic Association between SNP Markers and Diseases in the “Curraleiro Pé-Duro” Cattle |
| title_full_unstemmed | Genomic Association between SNP Markers and Diseases in the “Curraleiro Pé-Duro” Cattle |
| title_short | Genomic Association between SNP Markers and Diseases in the “Curraleiro Pé-Duro” Cattle |
| title_sort | genomic association between snp markers and diseases in the curraleiro pe duro cattle |
| topic | local breed polymorphism disease susceptibility |
| url | https://www.mdpi.com/2073-4425/12/6/806 |
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