Self-emergent vortex flow of microtubule and kinesin in cell-sized droplets under water/water phase separation
Abstract By facilitating a water/water phase separation (w/wPS), crowded biopolymers in cells form droplets that contribute to the spatial localization of biological components and their biochemical reactions. However, their influence on mechanical processes driven by protein motors has not been wel...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-04-01
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| Series: | Communications Chemistry |
| Online Access: | https://doi.org/10.1038/s42004-023-00879-5 |
| _version_ | 1797836531867582464 |
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| author | Hiroki Sakuta Naoki Nakatani Takayuki Torisawa Yutaka Sumino Kanta Tsumoto Kazuhiro Oiwa Kenichi Yoshikawa |
| author_facet | Hiroki Sakuta Naoki Nakatani Takayuki Torisawa Yutaka Sumino Kanta Tsumoto Kazuhiro Oiwa Kenichi Yoshikawa |
| author_sort | Hiroki Sakuta |
| collection | DOAJ |
| description | Abstract By facilitating a water/water phase separation (w/wPS), crowded biopolymers in cells form droplets that contribute to the spatial localization of biological components and their biochemical reactions. However, their influence on mechanical processes driven by protein motors has not been well studied. Here, we show that the w/wPS droplet spontaneously entraps kinesins as well as microtubules (MTs) and generates a micrometre-scale vortex flow inside the droplet. Active droplets with a size of 10–100 µm are generated through w/wPS of dextran and polyethylene glycol mixed with MTs, molecular-engineered chimeric four-headed kinesins and ATP after mechanical mixing. MTs and kinesin rapidly created contractile network accumulated at the interface of the droplet and gradually generated vortical flow, which can drive translational motion of a droplet. Our work reveals that the interface of w/wPS contributes not only to chemical processes but also produces mechanical motion by assembling species of protein motors in a functioning manner. |
| first_indexed | 2024-04-09T15:11:30Z |
| format | Article |
| id | doaj.art-218aea149e8d42d7a000c739c6d1103c |
| institution | Directory Open Access Journal |
| issn | 2399-3669 |
| language | English |
| last_indexed | 2024-04-09T15:11:30Z |
| publishDate | 2023-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Communications Chemistry |
| spelling | doaj.art-218aea149e8d42d7a000c739c6d1103c2023-04-30T11:09:52ZengNature PortfolioCommunications Chemistry2399-36692023-04-01611910.1038/s42004-023-00879-5Self-emergent vortex flow of microtubule and kinesin in cell-sized droplets under water/water phase separationHiroki Sakuta0Naoki Nakatani1Takayuki Torisawa2Yutaka Sumino3Kanta Tsumoto4Kazuhiro Oiwa5Kenichi Yoshikawa6Faculty of Life and Medical Sciences, Doshisha University, KyotanabeFaculty of Life and Medical Sciences, Doshisha University, KyotanabeCell Architecture Laboratory, Structural Biology Center, National Institute of Genetics, MishimaDepartment of Applied Physics, Faculty of Advanced Engineering, WaTUS and DCIS, Tokyo University of Science, KatsushikaDivision of Chemistry for Materials, Graduate School of Engineering, Mie UniversityAdvanced ICT Research Institute, National Institute of Information and Communications Technology, KobeFaculty of Life and Medical Sciences, Doshisha University, KyotanabeAbstract By facilitating a water/water phase separation (w/wPS), crowded biopolymers in cells form droplets that contribute to the spatial localization of biological components and their biochemical reactions. However, their influence on mechanical processes driven by protein motors has not been well studied. Here, we show that the w/wPS droplet spontaneously entraps kinesins as well as microtubules (MTs) and generates a micrometre-scale vortex flow inside the droplet. Active droplets with a size of 10–100 µm are generated through w/wPS of dextran and polyethylene glycol mixed with MTs, molecular-engineered chimeric four-headed kinesins and ATP after mechanical mixing. MTs and kinesin rapidly created contractile network accumulated at the interface of the droplet and gradually generated vortical flow, which can drive translational motion of a droplet. Our work reveals that the interface of w/wPS contributes not only to chemical processes but also produces mechanical motion by assembling species of protein motors in a functioning manner.https://doi.org/10.1038/s42004-023-00879-5 |
| spellingShingle | Hiroki Sakuta Naoki Nakatani Takayuki Torisawa Yutaka Sumino Kanta Tsumoto Kazuhiro Oiwa Kenichi Yoshikawa Self-emergent vortex flow of microtubule and kinesin in cell-sized droplets under water/water phase separation Communications Chemistry |
| title | Self-emergent vortex flow of microtubule and kinesin in cell-sized droplets under water/water phase separation |
| title_full | Self-emergent vortex flow of microtubule and kinesin in cell-sized droplets under water/water phase separation |
| title_fullStr | Self-emergent vortex flow of microtubule and kinesin in cell-sized droplets under water/water phase separation |
| title_full_unstemmed | Self-emergent vortex flow of microtubule and kinesin in cell-sized droplets under water/water phase separation |
| title_short | Self-emergent vortex flow of microtubule and kinesin in cell-sized droplets under water/water phase separation |
| title_sort | self emergent vortex flow of microtubule and kinesin in cell sized droplets under water water phase separation |
| url | https://doi.org/10.1038/s42004-023-00879-5 |
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