Metabolome and transcriptome associated analysis of sesquiterpenoid metabolism in Nardostachys jatamansi
BackgroundNardostachys jatamansi, an extremely endangered valuable plant of the alpine Himalayas, can synthesize specific sesquiterpenoids with multiple effective therapies and is widely exploited for the preparation of drugs, cosmetics and even religious functions (e.g., well-known spikenard). Howe...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.1041321/full |
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| author | Mingkang Feng Mingkang Feng Mingkang Feng Chen Chen Chen Chen Junzhang Qu-Bie Junzhang Qu-Bie Junzhang Qu-Bie Axiang Qu-Bie Axiang Qu-Bie Axiang Qu-Bie Xiaoming Bao Qi Cui Qi Cui Qi Cui Xinjia Yan Xinjia Yan Ying Li Yuan Liu Yuan Liu Shaoshan Zhang Shaoshan Zhang |
| author_facet | Mingkang Feng Mingkang Feng Mingkang Feng Chen Chen Chen Chen Junzhang Qu-Bie Junzhang Qu-Bie Junzhang Qu-Bie Axiang Qu-Bie Axiang Qu-Bie Axiang Qu-Bie Xiaoming Bao Qi Cui Qi Cui Qi Cui Xinjia Yan Xinjia Yan Ying Li Yuan Liu Yuan Liu Shaoshan Zhang Shaoshan Zhang |
| author_sort | Mingkang Feng |
| collection | DOAJ |
| description | BackgroundNardostachys jatamansi, an extremely endangered valuable plant of the alpine Himalayas, can synthesize specific sesquiterpenoids with multiple effective therapies and is widely exploited for the preparation of drugs, cosmetics and even religious functions (e.g., well-known spikenard). However, how accumulation trend of the sesquiterpenoids in tissues and the molecular mechanisms underlying the production of the active ingredients are not well understood.MethodsThe single-molecule real-time (SMRT) and RNA-seq transcriptome sequencing were combined to analyse the roots, rhizomes, leaves, flowers and anthocaulus of N. jatamansi. The phytochemical analysis was performed by gas chromatography‒mass spectrometry (GC‒MS) and ultrahigh-performance liquid chromatography (UPLC).ResultsA high-quality full-length reference transcriptome with 26,503 unigenes was generated for the first time. For volatile components, a total of sixty-five compounds were successfully identified, including fifty sesquiterpenoids. Their accumulation levels in five tissues were significantly varied, and most of the sesquiterpenoids were mainly enriched in roots and rhizomes. In addition, five aromatic compounds were only detected in flowers, which may help the plant attract insects for pollination. For nonvolatile ingredients, nardosinone-type sesquiterpenoids (nardosinone, kanshone C, and isonardosinone) were detected almost exclusively in roots and rhizomes. The candidate genes associated with sesquiterpenoid biosynthesis were identified by transcriptome analysis. Consistently, it was found that most biosynthesis genes were abundantly expressed in the roots and rhizomes according to the functional enrichment and expression patterns results. There was a positive correlation between the expression profile of genes related to the biosynthesis and the accumulation level of sesquiterpenoids in tissues. Gene family function analysis identified 28 NjTPSs and 43 NjCYPs that may be involved in the biosynthesis of the corresponding sesquiterpenoids. Furthermore, gene family functional analysis and gene coexpression network analysis revealed 28 NjTPSs and 43 NjCYPs associated with nardosinone-type sesquiterpenoid biosynthesis.ConclusionOur research results reveal the framework of sesquiterpenoids accumulation and biosynthesis in plant tissues and provide valuable support for further studies to elucidate the molecular mechanisms of sesquiterpenoid regulation and accumulation in N. jatamansi and will also contribute to the comprehensive utilization of this alpine plant. |
| first_indexed | 2024-04-13T13:10:29Z |
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| language | English |
| last_indexed | 2024-04-13T13:10:29Z |
| publishDate | 2022-11-01 |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-218c916986cb4953b6476872952166ea2022-12-22T02:45:38ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-11-011310.3389/fpls.2022.10413211041321Metabolome and transcriptome associated analysis of sesquiterpenoid metabolism in Nardostachys jatamansiMingkang Feng0Mingkang Feng1Mingkang Feng2Chen Chen3Chen Chen4Junzhang Qu-Bie5Junzhang Qu-Bie6Junzhang Qu-Bie7Axiang Qu-Bie8Axiang Qu-Bie9Axiang Qu-Bie10Xiaoming Bao11Qi Cui12Qi Cui13Qi Cui14Xinjia Yan15Xinjia Yan16Ying Li17Yuan Liu18Yuan Liu19Shaoshan Zhang20Shaoshan Zhang21Tibetan Plateau Ethnic Medicinal Resources Protection and Utilization Key Laboratory of National Ethnic Affairs Commission of the People's Republic of China, Southwest Minzu University, Chengdu, ChinaSichuan Provincial Qiang-Yi Medicinal Resources Protection and Utilization Technology and Engineering Laboratory, Southwest Minzu University, Chengdu, ChinaCollege of Pharmacy, Southwest Minzu University, Chengdu, ChinaTibetan Plateau Ethnic Medicinal Resources Protection and Utilization Key Laboratory of National Ethnic Affairs Commission of the People's Republic of China, Southwest Minzu University, Chengdu, ChinaSichuan Provincial Qiang-Yi Medicinal Resources Protection and Utilization Technology and Engineering Laboratory, Southwest Minzu University, Chengdu, ChinaTibetan Plateau Ethnic Medicinal Resources Protection and Utilization Key Laboratory of National Ethnic Affairs Commission of the People's Republic of China, Southwest Minzu University, Chengdu, ChinaSichuan Provincial Qiang-Yi Medicinal Resources Protection and Utilization Technology and Engineering Laboratory, Southwest Minzu University, Chengdu, ChinaCollege of Pharmacy, Southwest Minzu University, Chengdu, ChinaTibetan Plateau Ethnic Medicinal Resources Protection and Utilization Key Laboratory of National Ethnic Affairs Commission of the People's Republic of China, Southwest Minzu University, Chengdu, ChinaSichuan Provincial Qiang-Yi Medicinal Resources Protection and Utilization Technology and Engineering Laboratory, Southwest Minzu University, Chengdu, ChinaCollege of Pharmacy, Southwest Minzu University, Chengdu, ChinaAnalysis and Application Center, Shimadzu (China) Co., Ltd, Chengdu, ChinaTibetan Plateau Ethnic Medicinal Resources Protection and Utilization Key Laboratory of National Ethnic Affairs Commission of the People's Republic of China, Southwest Minzu University, Chengdu, ChinaSichuan Provincial Qiang-Yi Medicinal Resources Protection and Utilization Technology and Engineering Laboratory, Southwest Minzu University, Chengdu, ChinaCollege of Pharmacy, Southwest Minzu University, Chengdu, ChinaTibetan Plateau Ethnic Medicinal Resources Protection and Utilization Key Laboratory of National Ethnic Affairs Commission of the People's Republic of China, Southwest Minzu University, Chengdu, ChinaSichuan Provincial Qiang-Yi Medicinal Resources Protection and Utilization Technology and Engineering Laboratory, Southwest Minzu University, Chengdu, ChinaCollege of Pharmacy, Southwest Minzu University, Chengdu, ChinaTibetan Plateau Ethnic Medicinal Resources Protection and Utilization Key Laboratory of National Ethnic Affairs Commission of the People's Republic of China, Southwest Minzu University, Chengdu, ChinaSichuan Provincial Qiang-Yi Medicinal Resources Protection and Utilization Technology and Engineering Laboratory, Southwest Minzu University, Chengdu, ChinaTibetan Plateau Ethnic Medicinal Resources Protection and Utilization Key Laboratory of National Ethnic Affairs Commission of the People's Republic of China, Southwest Minzu University, Chengdu, ChinaSichuan Provincial Qiang-Yi Medicinal Resources Protection and Utilization Technology and Engineering Laboratory, Southwest Minzu University, Chengdu, ChinaBackgroundNardostachys jatamansi, an extremely endangered valuable plant of the alpine Himalayas, can synthesize specific sesquiterpenoids with multiple effective therapies and is widely exploited for the preparation of drugs, cosmetics and even religious functions (e.g., well-known spikenard). However, how accumulation trend of the sesquiterpenoids in tissues and the molecular mechanisms underlying the production of the active ingredients are not well understood.MethodsThe single-molecule real-time (SMRT) and RNA-seq transcriptome sequencing were combined to analyse the roots, rhizomes, leaves, flowers and anthocaulus of N. jatamansi. The phytochemical analysis was performed by gas chromatography‒mass spectrometry (GC‒MS) and ultrahigh-performance liquid chromatography (UPLC).ResultsA high-quality full-length reference transcriptome with 26,503 unigenes was generated for the first time. For volatile components, a total of sixty-five compounds were successfully identified, including fifty sesquiterpenoids. Their accumulation levels in five tissues were significantly varied, and most of the sesquiterpenoids were mainly enriched in roots and rhizomes. In addition, five aromatic compounds were only detected in flowers, which may help the plant attract insects for pollination. For nonvolatile ingredients, nardosinone-type sesquiterpenoids (nardosinone, kanshone C, and isonardosinone) were detected almost exclusively in roots and rhizomes. The candidate genes associated with sesquiterpenoid biosynthesis were identified by transcriptome analysis. Consistently, it was found that most biosynthesis genes were abundantly expressed in the roots and rhizomes according to the functional enrichment and expression patterns results. There was a positive correlation between the expression profile of genes related to the biosynthesis and the accumulation level of sesquiterpenoids in tissues. Gene family function analysis identified 28 NjTPSs and 43 NjCYPs that may be involved in the biosynthesis of the corresponding sesquiterpenoids. Furthermore, gene family functional analysis and gene coexpression network analysis revealed 28 NjTPSs and 43 NjCYPs associated with nardosinone-type sesquiterpenoid biosynthesis.ConclusionOur research results reveal the framework of sesquiterpenoids accumulation and biosynthesis in plant tissues and provide valuable support for further studies to elucidate the molecular mechanisms of sesquiterpenoid regulation and accumulation in N. jatamansi and will also contribute to the comprehensive utilization of this alpine plant.https://www.frontiersin.org/articles/10.3389/fpls.2022.1041321/fullNardostachys jatamansisesquiterpenoidtranscriptome sequencingbiosynthesis and accumulationterpene synthasecytochrome P450 |
| spellingShingle | Mingkang Feng Mingkang Feng Mingkang Feng Chen Chen Chen Chen Junzhang Qu-Bie Junzhang Qu-Bie Junzhang Qu-Bie Axiang Qu-Bie Axiang Qu-Bie Axiang Qu-Bie Xiaoming Bao Qi Cui Qi Cui Qi Cui Xinjia Yan Xinjia Yan Ying Li Yuan Liu Yuan Liu Shaoshan Zhang Shaoshan Zhang Metabolome and transcriptome associated analysis of sesquiterpenoid metabolism in Nardostachys jatamansi Frontiers in Plant Science Nardostachys jatamansi sesquiterpenoid transcriptome sequencing biosynthesis and accumulation terpene synthase cytochrome P450 |
| title | Metabolome and transcriptome associated analysis of sesquiterpenoid metabolism in Nardostachys jatamansi |
| title_full | Metabolome and transcriptome associated analysis of sesquiterpenoid metabolism in Nardostachys jatamansi |
| title_fullStr | Metabolome and transcriptome associated analysis of sesquiterpenoid metabolism in Nardostachys jatamansi |
| title_full_unstemmed | Metabolome and transcriptome associated analysis of sesquiterpenoid metabolism in Nardostachys jatamansi |
| title_short | Metabolome and transcriptome associated analysis of sesquiterpenoid metabolism in Nardostachys jatamansi |
| title_sort | metabolome and transcriptome associated analysis of sesquiterpenoid metabolism in nardostachys jatamansi |
| topic | Nardostachys jatamansi sesquiterpenoid transcriptome sequencing biosynthesis and accumulation terpene synthase cytochrome P450 |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.1041321/full |
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