Expansion of Myeloid Derived Suppressor Cells Contributes to Platelet Activation by L-Arginine Deprivation during SARS-CoV-2 Infection
Massive platelet activation and thrombotic events characterize severe COVID-19, highlighting their critical role in SARS-CoV-2-induced immunopathology. Since there is a well-described expansion of myeloid-derived suppressor cells (MDSC) in severe COVID-19, we evaluated their possible role in platele...
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MDPI AG
2021-08-01
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| Online Access: | https://www.mdpi.com/2073-4409/10/8/2111 |
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| author | Alessandra Sacchi Germana Grassi Stefania Notari Simona Gili Veronica Bordoni Eleonora Tartaglia Rita Casetti Eleonora Cimini Davide Mariotti Gabriele Garotto Alessia Beccacece Luisa Marchioni Michele Bibas Emanuele Nicastri Giuseppe Ippolito Chiara Agrati |
| author_facet | Alessandra Sacchi Germana Grassi Stefania Notari Simona Gili Veronica Bordoni Eleonora Tartaglia Rita Casetti Eleonora Cimini Davide Mariotti Gabriele Garotto Alessia Beccacece Luisa Marchioni Michele Bibas Emanuele Nicastri Giuseppe Ippolito Chiara Agrati |
| author_sort | Alessandra Sacchi |
| collection | DOAJ |
| description | Massive platelet activation and thrombotic events characterize severe COVID-19, highlighting their critical role in SARS-CoV-2-induced immunopathology. Since there is a well-described expansion of myeloid-derived suppressor cells (MDSC) in severe COVID-19, we evaluated their possible role in platelet activation during SARS-CoV-2 infection. During COVID-19, a lower plasmatic L-arginine level was observed compared to healthy donors, which correlated with MDSC frequency. Additionally, activated GPIIb/IIIa complex (PAC-1) expression was higher on platelets from severe COVID-19 patients compared to healthy controls and inversely correlated with L-arginine plasmatic concentration. Notably, MDSC were able to induce PAC-1 expression in vitro by reducing L-arginine concentration, indicating a direct role of PMN-MDSC in platelet activation. Accordingly, we found a positive correlation between ex vivo platelet PAC-1 expression and PMN-MDSC frequency. Overall, our data demonstrate the involvement of PMN-MDSC in triggering platelet activation during COVID-19, highlighting a novel role of MDSC in driving COVID-19 pathogenesis. |
| first_indexed | 2024-03-10T08:55:16Z |
| format | Article |
| id | doaj.art-218f0a080f1b402ea189986c93854094 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T08:55:16Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-218f0a080f1b402ea189986c938540942023-11-22T07:11:53ZengMDPI AGCells2073-44092021-08-01108211110.3390/cells10082111Expansion of Myeloid Derived Suppressor Cells Contributes to Platelet Activation by L-Arginine Deprivation during SARS-CoV-2 InfectionAlessandra Sacchi0Germana Grassi1Stefania Notari2Simona Gili3Veronica Bordoni4Eleonora Tartaglia5Rita Casetti6Eleonora Cimini7Davide Mariotti8Gabriele Garotto9Alessia Beccacece10Luisa Marchioni11Michele Bibas12Emanuele Nicastri13Giuseppe Ippolito14Chiara Agrati15Department of Epidemiology, Pre-Clinical Research and Advanced Diagnostic, National Institute for Infectious Diseases “Lazzaro Spallanzani”-IRCCS, Via Portuense, 292-00149 Rome, ItalyDepartment of Epidemiology, Pre-Clinical Research and Advanced Diagnostic, National Institute for Infectious Diseases “Lazzaro Spallanzani”-IRCCS, Via Portuense, 292-00149 Rome, ItalyDepartment of Epidemiology, Pre-Clinical Research and Advanced Diagnostic, National Institute for Infectious Diseases “Lazzaro Spallanzani”-IRCCS, Via Portuense, 292-00149 Rome, ItalyDepartment of Epidemiology, Pre-Clinical Research and Advanced Diagnostic, National Institute for Infectious Diseases “Lazzaro Spallanzani”-IRCCS, Via Portuense, 292-00149 Rome, ItalyDepartment of Epidemiology, Pre-Clinical Research and Advanced Diagnostic, National Institute for Infectious Diseases “Lazzaro Spallanzani”-IRCCS, Via Portuense, 292-00149 Rome, ItalyDepartment of Epidemiology, Pre-Clinical Research and Advanced Diagnostic, National Institute for Infectious Diseases “Lazzaro Spallanzani”-IRCCS, Via Portuense, 292-00149 Rome, ItalyDepartment of Epidemiology, Pre-Clinical Research and Advanced Diagnostic, National Institute for Infectious Diseases “Lazzaro Spallanzani”-IRCCS, Via Portuense, 292-00149 Rome, ItalyDepartment of Epidemiology, Pre-Clinical Research and Advanced Diagnostic, National Institute for Infectious Diseases “Lazzaro Spallanzani”-IRCCS, Via Portuense, 292-00149 Rome, ItalyDepartment of Epidemiology, Pre-Clinical Research and Advanced Diagnostic, National Institute for Infectious Diseases “Lazzaro Spallanzani”-IRCCS, Via Portuense, 292-00149 Rome, ItalyClinical Department, National Institute for Infectious Diseases “Lazzaro Spallanzani”-IRCCS, Via Portuense, 292-00149 Rome, ItalyClinical Department, National Institute for Infectious Diseases “Lazzaro Spallanzani”-IRCCS, Via Portuense, 292-00149 Rome, ItalyClinical Department, National Institute for Infectious Diseases “Lazzaro Spallanzani”-IRCCS, Via Portuense, 292-00149 Rome, ItalyClinical Department, National Institute for Infectious Diseases “Lazzaro Spallanzani”-IRCCS, Via Portuense, 292-00149 Rome, ItalyClinical Department, National Institute for Infectious Diseases “Lazzaro Spallanzani”-IRCCS, Via Portuense, 292-00149 Rome, ItalyNational Institute for Infectious Diseases “Lazzaro Spallanzani”-IRCCS, Via Portuense, 292-00149 Rome, ItalyDepartment of Epidemiology, Pre-Clinical Research and Advanced Diagnostic, National Institute for Infectious Diseases “Lazzaro Spallanzani”-IRCCS, Via Portuense, 292-00149 Rome, ItalyMassive platelet activation and thrombotic events characterize severe COVID-19, highlighting their critical role in SARS-CoV-2-induced immunopathology. Since there is a well-described expansion of myeloid-derived suppressor cells (MDSC) in severe COVID-19, we evaluated their possible role in platelet activation during SARS-CoV-2 infection. During COVID-19, a lower plasmatic L-arginine level was observed compared to healthy donors, which correlated with MDSC frequency. Additionally, activated GPIIb/IIIa complex (PAC-1) expression was higher on platelets from severe COVID-19 patients compared to healthy controls and inversely correlated with L-arginine plasmatic concentration. Notably, MDSC were able to induce PAC-1 expression in vitro by reducing L-arginine concentration, indicating a direct role of PMN-MDSC in platelet activation. Accordingly, we found a positive correlation between ex vivo platelet PAC-1 expression and PMN-MDSC frequency. Overall, our data demonstrate the involvement of PMN-MDSC in triggering platelet activation during COVID-19, highlighting a novel role of MDSC in driving COVID-19 pathogenesis.https://www.mdpi.com/2073-4409/10/8/2111COVID-19MDSCplateletL-Arginine |
| spellingShingle | Alessandra Sacchi Germana Grassi Stefania Notari Simona Gili Veronica Bordoni Eleonora Tartaglia Rita Casetti Eleonora Cimini Davide Mariotti Gabriele Garotto Alessia Beccacece Luisa Marchioni Michele Bibas Emanuele Nicastri Giuseppe Ippolito Chiara Agrati Expansion of Myeloid Derived Suppressor Cells Contributes to Platelet Activation by L-Arginine Deprivation during SARS-CoV-2 Infection Cells COVID-19 MDSC platelet L-Arginine |
| title | Expansion of Myeloid Derived Suppressor Cells Contributes to Platelet Activation by L-Arginine Deprivation during SARS-CoV-2 Infection |
| title_full | Expansion of Myeloid Derived Suppressor Cells Contributes to Platelet Activation by L-Arginine Deprivation during SARS-CoV-2 Infection |
| title_fullStr | Expansion of Myeloid Derived Suppressor Cells Contributes to Platelet Activation by L-Arginine Deprivation during SARS-CoV-2 Infection |
| title_full_unstemmed | Expansion of Myeloid Derived Suppressor Cells Contributes to Platelet Activation by L-Arginine Deprivation during SARS-CoV-2 Infection |
| title_short | Expansion of Myeloid Derived Suppressor Cells Contributes to Platelet Activation by L-Arginine Deprivation during SARS-CoV-2 Infection |
| title_sort | expansion of myeloid derived suppressor cells contributes to platelet activation by l arginine deprivation during sars cov 2 infection |
| topic | COVID-19 MDSC platelet L-Arginine |
| url | https://www.mdpi.com/2073-4409/10/8/2111 |
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