Selection of Reference Genes for Quantitative Real-time PCR during Flower Development in Tree Peony (Paeonia suffruticosa Andr.)
Tree peony (Paeonia suffruticosa) is a perennial plant indigenous to China known for its elegant and vibrantly colorful flowers. A few genes involved in petal pigmentation have been cloned in tree peony. However, to date, there have been few studies on the comparison and selection of stable referenc...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2016-04-01
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| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fpls.2016.00516/full |
| _version_ | 1828412686183956480 |
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| author | Jian eLi Jian eLi Jigang eHan Yonghong eHu Ji eYang |
| author_facet | Jian eLi Jian eLi Jigang eHan Yonghong eHu Ji eYang |
| author_sort | Jian eLi |
| collection | DOAJ |
| description | Tree peony (Paeonia suffruticosa) is a perennial plant indigenous to China known for its elegant and vibrantly colorful flowers. A few genes involved in petal pigmentation have been cloned in tree peony. However, to date, there have been few studies on the comparison and selection of stable reference genes for gene expression analysis by quantitative reverse-transcription PCR (qRT-PCR) in this species. In this study, 10 candidate reference genes were evaluated for the normalization of qRT-PCR in three tree peony cultivars. GAPDH and UBC were identified as the top two most stable reference genes in ‘Feng Dan’ and ‘Xi Shi’, and EF-1α/UBC was recommended to be the best combination for ‘Que Hao’. The expression stability of various reference genes differed across cultivars, suggesting that selection and validation of reliable reference genes for quantitative gene expression analysis was necessary not only for different species but also for different cultivars. The results provided a list of reference genes for further study on gene expression in P. suffruticosa. However, in any case, a preliminary check on the accuracy of the best performing reference genes is requested for each qRT-PCR experiment. |
| first_indexed | 2024-12-10T12:53:18Z |
| format | Article |
| id | doaj.art-218fb18d16df45448552f8d7011c43f0 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-10T12:53:18Z |
| publishDate | 2016-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-218fb18d16df45448552f8d7011c43f02022-12-22T01:48:09ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2016-04-01710.3389/fpls.2016.00516178178Selection of Reference Genes for Quantitative Real-time PCR during Flower Development in Tree Peony (Paeonia suffruticosa Andr.)Jian eLi0Jian eLi1Jigang eHan2Yonghong eHu3Ji eYang4Fudan UniversityShanghai Chenshan Botanical GardenShanghai Chenshan Botanical GardenShanghai Chenshan Botanical GardenFudan UniversityTree peony (Paeonia suffruticosa) is a perennial plant indigenous to China known for its elegant and vibrantly colorful flowers. A few genes involved in petal pigmentation have been cloned in tree peony. However, to date, there have been few studies on the comparison and selection of stable reference genes for gene expression analysis by quantitative reverse-transcription PCR (qRT-PCR) in this species. In this study, 10 candidate reference genes were evaluated for the normalization of qRT-PCR in three tree peony cultivars. GAPDH and UBC were identified as the top two most stable reference genes in ‘Feng Dan’ and ‘Xi Shi’, and EF-1α/UBC was recommended to be the best combination for ‘Que Hao’. The expression stability of various reference genes differed across cultivars, suggesting that selection and validation of reliable reference genes for quantitative gene expression analysis was necessary not only for different species but also for different cultivars. The results provided a list of reference genes for further study on gene expression in P. suffruticosa. However, in any case, a preliminary check on the accuracy of the best performing reference genes is requested for each qRT-PCR experiment.http://journal.frontiersin.org/Journal/10.3389/fpls.2016.00516/fullqRT-PCRflower developmenttree peonyReference genesGene expression normalization |
| spellingShingle | Jian eLi Jian eLi Jigang eHan Yonghong eHu Ji eYang Selection of Reference Genes for Quantitative Real-time PCR during Flower Development in Tree Peony (Paeonia suffruticosa Andr.) Frontiers in Plant Science qRT-PCR flower development tree peony Reference genes Gene expression normalization |
| title | Selection of Reference Genes for Quantitative Real-time PCR during Flower Development in Tree Peony (Paeonia suffruticosa Andr.) |
| title_full | Selection of Reference Genes for Quantitative Real-time PCR during Flower Development in Tree Peony (Paeonia suffruticosa Andr.) |
| title_fullStr | Selection of Reference Genes for Quantitative Real-time PCR during Flower Development in Tree Peony (Paeonia suffruticosa Andr.) |
| title_full_unstemmed | Selection of Reference Genes for Quantitative Real-time PCR during Flower Development in Tree Peony (Paeonia suffruticosa Andr.) |
| title_short | Selection of Reference Genes for Quantitative Real-time PCR during Flower Development in Tree Peony (Paeonia suffruticosa Andr.) |
| title_sort | selection of reference genes for quantitative real time pcr during flower development in tree peony paeonia suffruticosa andr |
| topic | qRT-PCR flower development tree peony Reference genes Gene expression normalization |
| url | http://journal.frontiersin.org/Journal/10.3389/fpls.2016.00516/full |
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