Relationship between Nutrient Intake and Human Gut Microbiota in Monozygotic Twins
<i>Background and Objectives</i>: The gut microbiota is associated with human health and dietary nutrition. Various studies have been reported in this regard, but it is difficult to clearly analyze human gut microbiota as individual differences are significant. The causes of these indivi...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-03-01
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| Series: | Medicina |
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| Online Access: | https://www.mdpi.com/1648-9144/57/3/275 |
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| author | Natsuko Matsumoto Jonguk Park Rie Tomizawa Hitoshi Kawashima Koji Hosomi Kenji Mizuguchi Chika Honda Ritsuko Ozaki Yoshinori Iwatani Mikio Watanabe Jun Kunisawa |
| author_facet | Natsuko Matsumoto Jonguk Park Rie Tomizawa Hitoshi Kawashima Koji Hosomi Kenji Mizuguchi Chika Honda Ritsuko Ozaki Yoshinori Iwatani Mikio Watanabe Jun Kunisawa |
| author_sort | Natsuko Matsumoto |
| collection | DOAJ |
| description | <i>Background and Objectives</i>: The gut microbiota is associated with human health and dietary nutrition. Various studies have been reported in this regard, but it is difficult to clearly analyze human gut microbiota as individual differences are significant. The causes of these individual differences in intestinal microflora are genetic and/or environmental. In this study, we focused on differences between identical twins in Japan to clarify the effects of nutrients consumed on the entire gut microbiome, while excluding genetic differences. <i>Materials and Methods</i>: We selected healthy Japanese monozygotic twins for the study and confirmed their zygosity by matching 15 short tandem repeat loci. Their fecal samples were subjected to 16S rRNA sequencing and bioinformatics analyses to identify and compare the fluctuations in intestinal bacteria. <i>Results</i>: We identified 12 genera sensitive to environmental factors, and found that <i>Lactobacillus</i> was relatively unaffected by environmental factors. Moreover, we identified protein, fat, and some nutrient intake that can affect 12 genera, which have been identified to be more sensitive to environmental factors. Among the 12 genera, <i>Bacteroides</i> had a positive correlation with retinol equivalent intake (<i>rs</i> = 0.38), <i>Lachnospira</i> had a significantly negative correlation with protein, sodium, iron, vitamin D, vitamin B6, and vitamin B12 intake (<i>rs</i> = −0.38, −0.41, −0.39, −0.63, −0.42, −0.49, respectively), <i>Lachnospiraceae</i> ND3007 group had a positive correlation with fat intake (<i>rs</i> = 0.39), and <i>Lachnospiraceae</i> UCG-008 group had a negative correlation with the saturated fatty acid intake (<i>rs</i> = −0.45). <i>Conclusions</i>: Our study is the first to focus on the relationship between human gut microbiota and nutrient intake using samples from Japanese twins to exclude the effects of genetic factors. These findings will broaden our understanding of the more intuitive relationship between nutrient intake and the gut microbiota and can be a useful basis for finding useful biomarkers that contribute to human health. |
| first_indexed | 2024-03-10T13:10:46Z |
| format | Article |
| id | doaj.art-218fbdd43ba84d79ba65d9a6356fab1b |
| institution | Directory Open Access Journal |
| issn | 1010-660X 1648-9144 |
| language | English |
| last_indexed | 2024-03-10T13:10:46Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Medicina |
| spelling | doaj.art-218fbdd43ba84d79ba65d9a6356fab1b2023-11-21T10:45:45ZengMDPI AGMedicina1010-660X1648-91442021-03-0157327510.3390/medicina57030275Relationship between Nutrient Intake and Human Gut Microbiota in Monozygotic TwinsNatsuko Matsumoto0Jonguk Park1Rie Tomizawa2Hitoshi Kawashima3Koji Hosomi4Kenji Mizuguchi5Chika Honda6Ritsuko Ozaki7Yoshinori Iwatani8Mikio Watanabe9Jun Kunisawa10Department of Clinical Laboratory and Biomedical Sciences, Division of Health Sciences, Osaka University Graduate School of Medicine, Osaka 565-0871, JapanLaboratory of Bioinformatics, Artificial Intelligence Center for Health and Biomedical Research, National Institutes of Biomedical Innovation, Health and Nutrition, Osaka 567-0085, JapanCenter for Twin Research, Osaka University Graduate School of Medicine, Osaka 565-0871, JapanLaboratory of Bioinformatics, Artificial Intelligence Center for Health and Biomedical Research, National Institutes of Biomedical Innovation, Health and Nutrition, Osaka 567-0085, JapanCenter for Twin Research, Osaka University Graduate School of Medicine, Osaka 565-0871, JapanLaboratory of Bioinformatics, Artificial Intelligence Center for Health and Biomedical Research, National Institutes of Biomedical Innovation, Health and Nutrition, Osaka 567-0085, JapanCenter for Twin Research, Osaka University Graduate School of Medicine, Osaka 565-0871, JapanDepartment of Clinical Laboratory and Biomedical Sciences, Division of Health Sciences, Osaka University Graduate School of Medicine, Osaka 565-0871, JapanCenter for Twin Research, Osaka University Graduate School of Medicine, Osaka 565-0871, JapanDepartment of Clinical Laboratory and Biomedical Sciences, Division of Health Sciences, Osaka University Graduate School of Medicine, Osaka 565-0871, JapanCenter for Twin Research, Osaka University Graduate School of Medicine, Osaka 565-0871, Japan<i>Background and Objectives</i>: The gut microbiota is associated with human health and dietary nutrition. Various studies have been reported in this regard, but it is difficult to clearly analyze human gut microbiota as individual differences are significant. The causes of these individual differences in intestinal microflora are genetic and/or environmental. In this study, we focused on differences between identical twins in Japan to clarify the effects of nutrients consumed on the entire gut microbiome, while excluding genetic differences. <i>Materials and Methods</i>: We selected healthy Japanese monozygotic twins for the study and confirmed their zygosity by matching 15 short tandem repeat loci. Their fecal samples were subjected to 16S rRNA sequencing and bioinformatics analyses to identify and compare the fluctuations in intestinal bacteria. <i>Results</i>: We identified 12 genera sensitive to environmental factors, and found that <i>Lactobacillus</i> was relatively unaffected by environmental factors. Moreover, we identified protein, fat, and some nutrient intake that can affect 12 genera, which have been identified to be more sensitive to environmental factors. Among the 12 genera, <i>Bacteroides</i> had a positive correlation with retinol equivalent intake (<i>rs</i> = 0.38), <i>Lachnospira</i> had a significantly negative correlation with protein, sodium, iron, vitamin D, vitamin B6, and vitamin B12 intake (<i>rs</i> = −0.38, −0.41, −0.39, −0.63, −0.42, −0.49, respectively), <i>Lachnospiraceae</i> ND3007 group had a positive correlation with fat intake (<i>rs</i> = 0.39), and <i>Lachnospiraceae</i> UCG-008 group had a negative correlation with the saturated fatty acid intake (<i>rs</i> = −0.45). <i>Conclusions</i>: Our study is the first to focus on the relationship between human gut microbiota and nutrient intake using samples from Japanese twins to exclude the effects of genetic factors. These findings will broaden our understanding of the more intuitive relationship between nutrient intake and the gut microbiota and can be a useful basis for finding useful biomarkers that contribute to human health.https://www.mdpi.com/1648-9144/57/3/275monozygotic twinsgut microbiomenutrients |
| spellingShingle | Natsuko Matsumoto Jonguk Park Rie Tomizawa Hitoshi Kawashima Koji Hosomi Kenji Mizuguchi Chika Honda Ritsuko Ozaki Yoshinori Iwatani Mikio Watanabe Jun Kunisawa Relationship between Nutrient Intake and Human Gut Microbiota in Monozygotic Twins Medicina monozygotic twins gut microbiome nutrients |
| title | Relationship between Nutrient Intake and Human Gut Microbiota in Monozygotic Twins |
| title_full | Relationship between Nutrient Intake and Human Gut Microbiota in Monozygotic Twins |
| title_fullStr | Relationship between Nutrient Intake and Human Gut Microbiota in Monozygotic Twins |
| title_full_unstemmed | Relationship between Nutrient Intake and Human Gut Microbiota in Monozygotic Twins |
| title_short | Relationship between Nutrient Intake and Human Gut Microbiota in Monozygotic Twins |
| title_sort | relationship between nutrient intake and human gut microbiota in monozygotic twins |
| topic | monozygotic twins gut microbiome nutrients |
| url | https://www.mdpi.com/1648-9144/57/3/275 |
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