TFF1 inhibits proliferation and induces apoptosis of gastric cancer cells in vitro
Trefoil Factor Family (TFF) plays an essential role in the intestinal epithelial restitution, but the relationship between TFF1 and gastric cancer (GC) is still unclear. The present study aimed to determine the role of TFF1 in repairing gastric mucosa and in the pathogenesis of GC. The TFF1 expres...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina
2012-05-01
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| Series: | Biomolecules & Biomedicine |
| Subjects: | |
| Online Access: | https://www.bjbms.org/ojs/index.php/bjbms/article/view/2499 |
| _version_ | 1797260868539383808 |
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| author | Yanli Ge Junjie Zhang Jianchun Cao Qiong Wu Longe Sun Likun Guo Zhirong Wang |
| author_facet | Yanli Ge Junjie Zhang Jianchun Cao Qiong Wu Longe Sun Likun Guo Zhirong Wang |
| author_sort | Yanli Ge |
| collection | DOAJ |
| description | Trefoil Factor Family (TFF) plays an essential role in the intestinal epithelial restitution, but the relationship between TFF1 and gastric cancer (GC) is still unclear. The present study aimed to determine the role of TFF1 in repairing gastric mucosa and in the pathogenesis of GC.
The TFF1 expression in different gastric mucosas was measured with immunohistochemistry. Then, siRNA targeting TFF1 or plasmids expressing TFF1 gene were transfected into BGC823 cells, SGC7901 cells and GES-1 cells. The cell proliferation was detected with MTT assay and apoptosis and cell cycle measured by flow cytometry.
From normal gastric mucosa to mucosa with dysplasia and to gastric cancer, the TFF1 expression had a decreasing trend. Down-regulation of TFF1 expression significantly reduced the apoptosis of three cell lines and markedly facilitated their proliferation but had no significant effect on cell cycle. Over-expression of TFF1 could promote apoptosis of three cell lines and inhibit proliferation but had no pronounced effect on cell cycle. TFF1 can inhibit proliferation and induce apoptosis of GC cells in vitro.
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| first_indexed | 2024-04-24T23:32:10Z |
| format | Article |
| id | doaj.art-219022fb11d843bda0bfbd19e0d4f532 |
| institution | Directory Open Access Journal |
| issn | 2831-0896 2831-090X |
| language | English |
| last_indexed | 2024-04-24T23:32:10Z |
| publishDate | 2012-05-01 |
| publisher | Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina |
| record_format | Article |
| series | Biomolecules & Biomedicine |
| spelling | doaj.art-219022fb11d843bda0bfbd19e0d4f5322024-03-15T14:33:49ZengAssociation of Basic Medical Sciences of Federation of Bosnia and HerzegovinaBiomolecules & Biomedicine2831-08962831-090X2012-05-0112210.17305/bjbms.2012.2499291TFF1 inhibits proliferation and induces apoptosis of gastric cancer cells in vitroYanli Ge0Junjie Zhang1Jianchun Cao2Qiong Wu3Longe Sun4Likun Guo5Zhirong Wang6Department of Gastroenterology, Tongji Hospital, Tongji UniversityDepartment of Gastroenterology, Tongji Hospital, Tongji UniversityDepartment of Gastroenterology, Tongji Hospital, Tongji UniversityDepartment of Gastroenterology, Tongji Hospital, Tongji UniversityDepartment of Gastroenterology, Tongji Hospital, Tongji UniversityDepartment of Gastroenterology, Tongji Hospital, Tongji UniversityDepartment of Gastroenterology, Tongji Hospital, Tongji UniversityTrefoil Factor Family (TFF) plays an essential role in the intestinal epithelial restitution, but the relationship between TFF1 and gastric cancer (GC) is still unclear. The present study aimed to determine the role of TFF1 in repairing gastric mucosa and in the pathogenesis of GC. The TFF1 expression in different gastric mucosas was measured with immunohistochemistry. Then, siRNA targeting TFF1 or plasmids expressing TFF1 gene were transfected into BGC823 cells, SGC7901 cells and GES-1 cells. The cell proliferation was detected with MTT assay and apoptosis and cell cycle measured by flow cytometry. From normal gastric mucosa to mucosa with dysplasia and to gastric cancer, the TFF1 expression had a decreasing trend. Down-regulation of TFF1 expression significantly reduced the apoptosis of three cell lines and markedly facilitated their proliferation but had no significant effect on cell cycle. Over-expression of TFF1 could promote apoptosis of three cell lines and inhibit proliferation but had no pronounced effect on cell cycle. TFF1 can inhibit proliferation and induce apoptosis of GC cells in vitro. https://www.bjbms.org/ojs/index.php/bjbms/article/view/2499Trefoil Factor Family 1gastric cancerapoptosisin vitro |
| spellingShingle | Yanli Ge Junjie Zhang Jianchun Cao Qiong Wu Longe Sun Likun Guo Zhirong Wang TFF1 inhibits proliferation and induces apoptosis of gastric cancer cells in vitro Biomolecules & Biomedicine Trefoil Factor Family 1 gastric cancer apoptosis in vitro |
| title | TFF1 inhibits proliferation and induces apoptosis of gastric cancer cells in vitro |
| title_full | TFF1 inhibits proliferation and induces apoptosis of gastric cancer cells in vitro |
| title_fullStr | TFF1 inhibits proliferation and induces apoptosis of gastric cancer cells in vitro |
| title_full_unstemmed | TFF1 inhibits proliferation and induces apoptosis of gastric cancer cells in vitro |
| title_short | TFF1 inhibits proliferation and induces apoptosis of gastric cancer cells in vitro |
| title_sort | tff1 inhibits proliferation and induces apoptosis of gastric cancer cells in vitro |
| topic | Trefoil Factor Family 1 gastric cancer apoptosis in vitro |
| url | https://www.bjbms.org/ojs/index.php/bjbms/article/view/2499 |
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