Oncogenic mutations of PIK3CA and HRAS in carcinoma of cervix in South Indian women
Carcinoma of the cervix is the second most successive reason of female cancer which remains an imperative medical issue in women around the world. Mutations are normally dissected by tissue biopsy which is intrusive, costly and possibly subjective. However, detection of mutations from blood is a non...
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| Format: | Article |
| Language: | English |
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Turkiye Klinikleri
2017-12-01
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| Series: | Journal of Oncological Sciences |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2452336417300584 |
| _version_ | 1797913042440159232 |
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| author | Geetha kumari Konathala Ramesh Mandarapu Sudhakar Godi |
| author_facet | Geetha kumari Konathala Ramesh Mandarapu Sudhakar Godi |
| author_sort | Geetha kumari Konathala |
| collection | DOAJ |
| description | Carcinoma of the cervix is the second most successive reason of female cancer which remains an imperative medical issue in women around the world. Mutations are normally dissected by tissue biopsy which is intrusive, costly and possibly subjective. However, detection of mutations from blood is a non-invasive procedure. However, detection of mutations from blood is a non-invasive procedure. It would offer several advantages, including greater speed and cheap at a cost such that the family members can likewise be screened. Repeated tests after surgery provide an early cautioning for the disease recurrence. The present work aimed to assess the frequency of PIK3CA and HRAS mutations in cervical cancer patients using peripheral blood. This study includes 210 cases presenting cervical cancer and 210 age and sex-matched healthy controls. Genomic DNA was isolated from peripheral blood. The PCR-CTPP method was performed for screening of exons 9 and 20 of PIK3CA and exon 34 HRAS mutations. Sequencing of PIK3CA and of HRAS genes was done for further confirmation. Out of eight mutations studied, no clear disease causing mutations were noticed in any of the cervical cancer patients in the present investigation. Cervical cancer harbors excessive rates of targetable oncogenic mutations. We couldn't find any changes in our investigation. If a connection amongst tissue and non-tissue mutations could be shown, the probability of a basic blood test to distinguish possibility for anticancer treatment comes a bit nearer. Repeated blood tests after surgery provide an early cautioning for disease recurrence. Therefore, in future evaluation of a larger data set and cases with progressive tumor (Stage III–IV) will be required to approve these findings. |
| first_indexed | 2024-04-10T12:05:32Z |
| format | Article |
| id | doaj.art-2190de70fb5d48ca9465fafc6cb17746 |
| institution | Directory Open Access Journal |
| issn | 2452-3364 |
| language | English |
| last_indexed | 2024-04-10T12:05:32Z |
| publishDate | 2017-12-01 |
| publisher | Turkiye Klinikleri |
| record_format | Article |
| series | Journal of Oncological Sciences |
| spelling | doaj.art-2190de70fb5d48ca9465fafc6cb177462023-02-15T16:16:18ZengTurkiye KlinikleriJournal of Oncological Sciences2452-33642017-12-013311211610.1016/j.jons.2017.10.004Oncogenic mutations of PIK3CA and HRAS in carcinoma of cervix in South Indian womenGeetha kumari KonathalaRamesh MandarapuSudhakar GodiCarcinoma of the cervix is the second most successive reason of female cancer which remains an imperative medical issue in women around the world. Mutations are normally dissected by tissue biopsy which is intrusive, costly and possibly subjective. However, detection of mutations from blood is a non-invasive procedure. However, detection of mutations from blood is a non-invasive procedure. It would offer several advantages, including greater speed and cheap at a cost such that the family members can likewise be screened. Repeated tests after surgery provide an early cautioning for the disease recurrence. The present work aimed to assess the frequency of PIK3CA and HRAS mutations in cervical cancer patients using peripheral blood. This study includes 210 cases presenting cervical cancer and 210 age and sex-matched healthy controls. Genomic DNA was isolated from peripheral blood. The PCR-CTPP method was performed for screening of exons 9 and 20 of PIK3CA and exon 34 HRAS mutations. Sequencing of PIK3CA and of HRAS genes was done for further confirmation. Out of eight mutations studied, no clear disease causing mutations were noticed in any of the cervical cancer patients in the present investigation. Cervical cancer harbors excessive rates of targetable oncogenic mutations. We couldn't find any changes in our investigation. If a connection amongst tissue and non-tissue mutations could be shown, the probability of a basic blood test to distinguish possibility for anticancer treatment comes a bit nearer. Repeated blood tests after surgery provide an early cautioning for disease recurrence. Therefore, in future evaluation of a larger data set and cases with progressive tumor (Stage III–IV) will be required to approve these findings.http://www.sciencedirect.com/science/article/pii/S2452336417300584Oncogenic mutationsCervical cancerPeripheral bloodPCR-CTPP method |
| spellingShingle | Geetha kumari Konathala Ramesh Mandarapu Sudhakar Godi Oncogenic mutations of PIK3CA and HRAS in carcinoma of cervix in South Indian women Journal of Oncological Sciences Oncogenic mutations Cervical cancer Peripheral blood PCR-CTPP method |
| title | Oncogenic mutations of PIK3CA and HRAS in carcinoma of cervix in South Indian women |
| title_full | Oncogenic mutations of PIK3CA and HRAS in carcinoma of cervix in South Indian women |
| title_fullStr | Oncogenic mutations of PIK3CA and HRAS in carcinoma of cervix in South Indian women |
| title_full_unstemmed | Oncogenic mutations of PIK3CA and HRAS in carcinoma of cervix in South Indian women |
| title_short | Oncogenic mutations of PIK3CA and HRAS in carcinoma of cervix in South Indian women |
| title_sort | oncogenic mutations of pik3ca and hras in carcinoma of cervix in south indian women |
| topic | Oncogenic mutations Cervical cancer Peripheral blood PCR-CTPP method |
| url | http://www.sciencedirect.com/science/article/pii/S2452336417300584 |
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