REACTION OF THE CIRCULATING REGULATORY T CELLS AFTER CHEMORADIATION THERAPY OF HODGKIN LYMPHOMA
Objective: Purpose of the research is to determine the reaction of regulatory T cells after chemoradiation therapy of Hodgkin lymphoma. Methodology: 29 samples of peripheral blood of patients with Hodgkin lymphoma (before treatment – 10; after chemotherapy – 9; after consolidation radiotherapy – 10)...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-10-01
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| Series: | Hematology, Transfusion and Cell Therapy |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2531137923002365 |
| _version_ | 1827797194989633536 |
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| author | Tatiana Mushkarina Evgenija Kuzmina Tatiana Bogatyreva Ludmila Grivtsova |
| author_facet | Tatiana Mushkarina Evgenija Kuzmina Tatiana Bogatyreva Ludmila Grivtsova |
| author_sort | Tatiana Mushkarina |
| collection | DOAJ |
| description | Objective: Purpose of the research is to determine the reaction of regulatory T cells after chemoradiation therapy of Hodgkin lymphoma. Methodology: 29 samples of peripheral blood of patients with Hodgkin lymphoma (before treatment – 10; after chemotherapy – 9; after consolidation radiotherapy – 10). Chemotherapy was carried out according to the following schemes: ABVD, BEACOPP with the addition of 1-2 courses of СVРР or СОРР. The subsequent consolidation of radiation therapy was accomplished to a dose of 20-24 Gy. Тreg-cells were identified by phenotype CD45+CD4+CD25+CD127-. Control group consisted of 40 practically healthy people. The group data were compared using the Mann-Whitney U test. Results: At the onset of Hodgkin lymphoma the percentage and absolute count of regulatory T cells corresponded to normal values (5.19%/0.036*109 cells/l - Hodgkin lymphoma vs 3.69%/0.031*109 cells/l - control level, p>0.05). After chemotherapy the percentage of regulatory T cells increased to 9.09%, p<0.05; the absolute count remained at the same level (0.037*109 cells/l, p>0.05). After consolidation of radiation therapy the percentage of regulatory T cells was determined at the level of 9.19%, p>0.05. The decrease of absolute count of regulatory T cells was statistically significant difference and was near 0.019*109 cells/l. Conclusion: There is a relative redistribution of cells within a subpopulation of activated CD4+CD25+T cells towards an increase in the level of regulatory T cells after chemotherapy of Hodgkin lymphoma. The subsequent radiotherapy consolidation at a dose of 20-24 Gy continued to increase the sensitivity of regulatory T cells to the radiation component of chemoradiation therapy. |
| first_indexed | 2024-03-11T19:18:51Z |
| format | Article |
| id | doaj.art-21930b5773b44c53bfc649fcb8ec0eb8 |
| institution | Directory Open Access Journal |
| issn | 2531-1379 |
| language | English |
| last_indexed | 2024-03-11T19:18:51Z |
| publishDate | 2023-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Hematology, Transfusion and Cell Therapy |
| spelling | doaj.art-21930b5773b44c53bfc649fcb8ec0eb82023-10-08T04:35:52ZengElsevierHematology, Transfusion and Cell Therapy2531-13792023-10-0145S36REACTION OF THE CIRCULATING REGULATORY T CELLS AFTER CHEMORADIATION THERAPY OF HODGKIN LYMPHOMATatiana Mushkarina0Evgenija Kuzmina1Tatiana Bogatyreva2Ludmila Grivtsova3A. Tsyb Medical Radiological Research Centre MRRCA. Tsyb Medical Radiological Research Centre MRRCA. Tsyb Medical Radiological Research Centre MRRCA. Tsyb Medical Radiological Research Centre MRRCObjective: Purpose of the research is to determine the reaction of regulatory T cells after chemoradiation therapy of Hodgkin lymphoma. Methodology: 29 samples of peripheral blood of patients with Hodgkin lymphoma (before treatment – 10; after chemotherapy – 9; after consolidation radiotherapy – 10). Chemotherapy was carried out according to the following schemes: ABVD, BEACOPP with the addition of 1-2 courses of СVРР or СОРР. The subsequent consolidation of radiation therapy was accomplished to a dose of 20-24 Gy. Тreg-cells were identified by phenotype CD45+CD4+CD25+CD127-. Control group consisted of 40 practically healthy people. The group data were compared using the Mann-Whitney U test. Results: At the onset of Hodgkin lymphoma the percentage and absolute count of regulatory T cells corresponded to normal values (5.19%/0.036*109 cells/l - Hodgkin lymphoma vs 3.69%/0.031*109 cells/l - control level, p>0.05). After chemotherapy the percentage of regulatory T cells increased to 9.09%, p<0.05; the absolute count remained at the same level (0.037*109 cells/l, p>0.05). After consolidation of radiation therapy the percentage of regulatory T cells was determined at the level of 9.19%, p>0.05. The decrease of absolute count of regulatory T cells was statistically significant difference and was near 0.019*109 cells/l. Conclusion: There is a relative redistribution of cells within a subpopulation of activated CD4+CD25+T cells towards an increase in the level of regulatory T cells after chemotherapy of Hodgkin lymphoma. The subsequent radiotherapy consolidation at a dose of 20-24 Gy continued to increase the sensitivity of regulatory T cells to the radiation component of chemoradiation therapy.http://www.sciencedirect.com/science/article/pii/S2531137923002365 |
| spellingShingle | Tatiana Mushkarina Evgenija Kuzmina Tatiana Bogatyreva Ludmila Grivtsova REACTION OF THE CIRCULATING REGULATORY T CELLS AFTER CHEMORADIATION THERAPY OF HODGKIN LYMPHOMA Hematology, Transfusion and Cell Therapy |
| title | REACTION OF THE CIRCULATING REGULATORY T CELLS AFTER CHEMORADIATION THERAPY OF HODGKIN LYMPHOMA |
| title_full | REACTION OF THE CIRCULATING REGULATORY T CELLS AFTER CHEMORADIATION THERAPY OF HODGKIN LYMPHOMA |
| title_fullStr | REACTION OF THE CIRCULATING REGULATORY T CELLS AFTER CHEMORADIATION THERAPY OF HODGKIN LYMPHOMA |
| title_full_unstemmed | REACTION OF THE CIRCULATING REGULATORY T CELLS AFTER CHEMORADIATION THERAPY OF HODGKIN LYMPHOMA |
| title_short | REACTION OF THE CIRCULATING REGULATORY T CELLS AFTER CHEMORADIATION THERAPY OF HODGKIN LYMPHOMA |
| title_sort | reaction of the circulating regulatory t cells after chemoradiation therapy of hodgkin lymphoma |
| url | http://www.sciencedirect.com/science/article/pii/S2531137923002365 |
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