Antigenicity and immunogenicity of different morphological forms of Borrelia burgdorferi sensu lato spirochetes
Abstract Borrelia burgdorferi sensu lato is a species complex of pleomorphic spirochetes, including species that cause Lyme disease (LD) in humans. In addition to classic spiral forms, these bacteria are capable of creating morphological forms referred to as round bodies and aggregates. The subject...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-02-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-54505-y |
| _version_ | 1797275125023768576 |
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| author | Kristyna Sloupenska Barbora Koubkova Pavel Horak Jana Dolezilkova Beata Hutyrova Mojmir Racansky Martina Miklusova Jan Mares Milan Raska Michal Krupka |
| author_facet | Kristyna Sloupenska Barbora Koubkova Pavel Horak Jana Dolezilkova Beata Hutyrova Mojmir Racansky Martina Miklusova Jan Mares Milan Raska Michal Krupka |
| author_sort | Kristyna Sloupenska |
| collection | DOAJ |
| description | Abstract Borrelia burgdorferi sensu lato is a species complex of pleomorphic spirochetes, including species that cause Lyme disease (LD) in humans. In addition to classic spiral forms, these bacteria are capable of creating morphological forms referred to as round bodies and aggregates. The subject of discussion is their possible contribution to the persistence of infection or post-infection symptoms in LD. This study investigates the immunological properties of these forms by monitoring reactivity with early (n = 30) and late stage (n = 30) LD patient sera and evaluating the immune response induced by vaccination of mice. In patient sera, we found a quantitative difference in reactivity with individual morphotypes, when aggregates were recognized most intensively, but the difference was statistically significant in only half of the tested strains. In post-vaccination mouse sera, we observed a statistically significant higher reactivity with antigens p83 and p25 (OspC) in mice vaccinated with aggregates compared to mice vaccinated with spiral forms. The importance of the particulate nature of the antigen for the induction of a Th1-directed response has also been demonstrated. In any of morphological forms, the possibility of inducing antibodies cross-reacting with human nuclear and myositis specific/associated autoantigens was not confirmed by vaccination of mice. |
| first_indexed | 2024-03-07T15:09:50Z |
| format | Article |
| id | doaj.art-21933d397dca45289db44ab2d440c04a |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-07T15:09:50Z |
| publishDate | 2024-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-21933d397dca45289db44ab2d440c04a2024-03-05T18:42:22ZengNature PortfolioScientific Reports2045-23222024-02-0114111310.1038/s41598-024-54505-yAntigenicity and immunogenicity of different morphological forms of Borrelia burgdorferi sensu lato spirochetesKristyna Sloupenska0Barbora Koubkova1Pavel Horak2Jana Dolezilkova3Beata Hutyrova4Mojmir Racansky5Martina Miklusova6Jan Mares7Milan Raska8Michal Krupka9Department of Immunology, Faculty of Medicine and Dentistry, Palacky University OlomoucDepartment of Allergology and Clinical Immunology, University Hospital OlomoucThird Department of Internal Medicine-Nephrology, Rheumatology and Endocrinology, University Hospital OlomoucLaboratory of Medical Parasitology and Zoology, Public Health Institute OstravaDepartment of Immunology, Faculty of Medicine and Dentistry, Palacky University OlomoucDepartment of Immunology, Faculty of Medicine and Dentistry, Palacky University OlomoucDepartment of Neurology, University Hospital OlomoucDepartment of Neurology, University Hospital OlomoucDepartment of Immunology, Faculty of Medicine and Dentistry, Palacky University OlomoucDepartment of Immunology, Faculty of Medicine and Dentistry, Palacky University OlomoucAbstract Borrelia burgdorferi sensu lato is a species complex of pleomorphic spirochetes, including species that cause Lyme disease (LD) in humans. In addition to classic spiral forms, these bacteria are capable of creating morphological forms referred to as round bodies and aggregates. The subject of discussion is their possible contribution to the persistence of infection or post-infection symptoms in LD. This study investigates the immunological properties of these forms by monitoring reactivity with early (n = 30) and late stage (n = 30) LD patient sera and evaluating the immune response induced by vaccination of mice. In patient sera, we found a quantitative difference in reactivity with individual morphotypes, when aggregates were recognized most intensively, but the difference was statistically significant in only half of the tested strains. In post-vaccination mouse sera, we observed a statistically significant higher reactivity with antigens p83 and p25 (OspC) in mice vaccinated with aggregates compared to mice vaccinated with spiral forms. The importance of the particulate nature of the antigen for the induction of a Th1-directed response has also been demonstrated. In any of morphological forms, the possibility of inducing antibodies cross-reacting with human nuclear and myositis specific/associated autoantigens was not confirmed by vaccination of mice.https://doi.org/10.1038/s41598-024-54505-y |
| spellingShingle | Kristyna Sloupenska Barbora Koubkova Pavel Horak Jana Dolezilkova Beata Hutyrova Mojmir Racansky Martina Miklusova Jan Mares Milan Raska Michal Krupka Antigenicity and immunogenicity of different morphological forms of Borrelia burgdorferi sensu lato spirochetes Scientific Reports |
| title | Antigenicity and immunogenicity of different morphological forms of Borrelia burgdorferi sensu lato spirochetes |
| title_full | Antigenicity and immunogenicity of different morphological forms of Borrelia burgdorferi sensu lato spirochetes |
| title_fullStr | Antigenicity and immunogenicity of different morphological forms of Borrelia burgdorferi sensu lato spirochetes |
| title_full_unstemmed | Antigenicity and immunogenicity of different morphological forms of Borrelia burgdorferi sensu lato spirochetes |
| title_short | Antigenicity and immunogenicity of different morphological forms of Borrelia burgdorferi sensu lato spirochetes |
| title_sort | antigenicity and immunogenicity of different morphological forms of borrelia burgdorferi sensu lato spirochetes |
| url | https://doi.org/10.1038/s41598-024-54505-y |
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