Expression of Cell Cycle Genes During Sesbania rostrata Stem Nodule Development
Upon infection of Sesbania rostrata with Azorhizobium caulinodans, nodules are formed on roots and stems. Stem nodules develop from abundantly distributed dormant root primordia. To acquire more insight into the meristem organization during stem nodule development, the expression patterns of a mitot...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
The American Phytopathological Society
1997-04-01
|
| Series: | Molecular Plant-Microbe Interactions |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI.1997.10.3.316 |
| _version_ | 1811287612666675200 |
|---|---|
| author | Sofie Goormachtig Marcio Alves-Ferreira Marc Van Montagu Gilbert Engler Marcelle Holsters |
| author_facet | Sofie Goormachtig Marcio Alves-Ferreira Marc Van Montagu Gilbert Engler Marcelle Holsters |
| author_sort | Sofie Goormachtig |
| collection | DOAJ |
| description | Upon infection of Sesbania rostrata with Azorhizobium caulinodans, nodules are formed on roots and stems. Stem nodules develop from abundantly distributed dormant root primordia. To acquire more insight into the meristem organization during stem nodule development, the expression patterns of a mitotic B1-type cyclin gene (Sesro; CycB1;1), a cyclin-dependent kinase gene (Cdc2-1Sr), and a histone H4 gene (H4-1Sr) of S. rostrata were followed by in situ hybridization. Cdc2-1Sr transcripts were found in all cells of uninfected and infected root primordia. In uninfected root primordia, Sesro;CycB1;1 transcripts were detected in a few cells of the apical root meristem whereas H4-1Sr transcripts were abundant in this region. Interestingly, after inoculation with A. caulinodans, H4-1Sr transcripts disappeared in the root meristem and a patchy pattern of Sesro;CycB1;1 and H4-1Sr expression appeared in the cortex of the root primordium, reflecting the formation of globular nodule primordia. When bacterial invasion started, a distal nodule meristem was delimited wherein Sesro;CycB1;1 and H4-1Sr expression was concentrated. Approximately 1 week after inoculation, meristem activity ceased, indicated by the loss of Sesro;CycB1;1 and H4-1Sr expression. |
| first_indexed | 2024-04-13T03:21:38Z |
| format | Article |
| id | doaj.art-219735847b9b417faff235902c8c65e6 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-13T03:21:38Z |
| publishDate | 1997-04-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-219735847b9b417faff235902c8c65e62022-12-22T03:04:45ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77061997-04-0110331632510.1094/MPMI.1997.10.3.316Expression of Cell Cycle Genes During Sesbania rostrata Stem Nodule DevelopmentSofie GoormachtigMarcio Alves-FerreiraMarc Van MontaguGilbert EnglerMarcelle HolstersUpon infection of Sesbania rostrata with Azorhizobium caulinodans, nodules are formed on roots and stems. Stem nodules develop from abundantly distributed dormant root primordia. To acquire more insight into the meristem organization during stem nodule development, the expression patterns of a mitotic B1-type cyclin gene (Sesro; CycB1;1), a cyclin-dependent kinase gene (Cdc2-1Sr), and a histone H4 gene (H4-1Sr) of S. rostrata were followed by in situ hybridization. Cdc2-1Sr transcripts were found in all cells of uninfected and infected root primordia. In uninfected root primordia, Sesro;CycB1;1 transcripts were detected in a few cells of the apical root meristem whereas H4-1Sr transcripts were abundant in this region. Interestingly, after inoculation with A. caulinodans, H4-1Sr transcripts disappeared in the root meristem and a patchy pattern of Sesro;CycB1;1 and H4-1Sr expression appeared in the cortex of the root primordium, reflecting the formation of globular nodule primordia. When bacterial invasion started, a distal nodule meristem was delimited wherein Sesro;CycB1;1 and H4-1Sr expression was concentrated. Approximately 1 week after inoculation, meristem activity ceased, indicated by the loss of Sesro;CycB1;1 and H4-1Sr expression.https://apsjournals.apsnet.org/doi/10.1094/MPMI.1997.10.3.316 |
| spellingShingle | Sofie Goormachtig Marcio Alves-Ferreira Marc Van Montagu Gilbert Engler Marcelle Holsters Expression of Cell Cycle Genes During Sesbania rostrata Stem Nodule Development Molecular Plant-Microbe Interactions |
| title | Expression of Cell Cycle Genes During Sesbania rostrata Stem Nodule Development |
| title_full | Expression of Cell Cycle Genes During Sesbania rostrata Stem Nodule Development |
| title_fullStr | Expression of Cell Cycle Genes During Sesbania rostrata Stem Nodule Development |
| title_full_unstemmed | Expression of Cell Cycle Genes During Sesbania rostrata Stem Nodule Development |
| title_short | Expression of Cell Cycle Genes During Sesbania rostrata Stem Nodule Development |
| title_sort | expression of cell cycle genes during sesbania rostrata stem nodule development |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI.1997.10.3.316 |
| work_keys_str_mv | AT sofiegoormachtig expressionofcellcyclegenesduringsesbaniarostratastemnoduledevelopment AT marcioalvesferreira expressionofcellcyclegenesduringsesbaniarostratastemnoduledevelopment AT marcvanmontagu expressionofcellcyclegenesduringsesbaniarostratastemnoduledevelopment AT gilbertengler expressionofcellcyclegenesduringsesbaniarostratastemnoduledevelopment AT marcelleholsters expressionofcellcyclegenesduringsesbaniarostratastemnoduledevelopment |