Rapid genotyping protocol to improve dengue virus serotype 2 survey in Lao PDR.
Dengue fever is one of the major public health problems in Lao PDR. Over the last decade, dengue virus (DENV) epidemics were characterized by a novel predominant serotype accompanied by at least two other serotypes. Since 2008, DENV-2 circulated at a low level in Lao PDR but its epidemiologic profil...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2020-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0237384 |
| _version_ | 1818586229165260800 |
|---|---|
| author | Elodie Calvez Somphavanh Somlor Souksakhone Viengphouthong Charlotte Balière Phaithong Bounmany Sitsana Keosenhom Valérie Caro Marc Grandadam |
| author_facet | Elodie Calvez Somphavanh Somlor Souksakhone Viengphouthong Charlotte Balière Phaithong Bounmany Sitsana Keosenhom Valérie Caro Marc Grandadam |
| author_sort | Elodie Calvez |
| collection | DOAJ |
| description | Dengue fever is one of the major public health problems in Lao PDR. Over the last decade, dengue virus (DENV) epidemics were characterized by a novel predominant serotype accompanied by at least two other serotypes. Since 2008, DENV-2 circulated at a low level in Lao PDR but its epidemiologic profile changed at the end of 2018. Indeed, the number of confirmed DENV-2 cases suddenly increased in October 2018 and DENV-2 became predominant at the country level in early 2019. We developed a Genotype Screening Protocol (GSP) to determine the origin(s) of the Lao DENV-2 and study their genetic polymorphism. With a good correlation with full envelope gene sequencing data, this molecular epidemiology tool evidence the co-circulation of two highly polymorphic DENV-2 genotypes, i.e. Asian I and Cosmopolitan genotypes, over the last five years, suggesting multiple introductions of DENV-2 in the country. GSP approach provides relevant first line information that may help countries with limited laboratory resources to reinforce their capabilities to DENV-2 and to follow the epidemics progresses and assess situations at the regional level. |
| first_indexed | 2024-12-16T08:49:38Z |
| format | Article |
| id | doaj.art-21973b0d8e694890a3d90ae62e690f1a |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-16T08:49:38Z |
| publishDate | 2020-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-21973b0d8e694890a3d90ae62e690f1a2022-12-21T22:37:30ZengPublic Library of Science (PLoS)PLoS ONE1932-62032020-01-01158e023738410.1371/journal.pone.0237384Rapid genotyping protocol to improve dengue virus serotype 2 survey in Lao PDR.Elodie CalvezSomphavanh SomlorSouksakhone ViengphouthongCharlotte BalièrePhaithong BounmanySitsana KeosenhomValérie CaroMarc GrandadamDengue fever is one of the major public health problems in Lao PDR. Over the last decade, dengue virus (DENV) epidemics were characterized by a novel predominant serotype accompanied by at least two other serotypes. Since 2008, DENV-2 circulated at a low level in Lao PDR but its epidemiologic profile changed at the end of 2018. Indeed, the number of confirmed DENV-2 cases suddenly increased in October 2018 and DENV-2 became predominant at the country level in early 2019. We developed a Genotype Screening Protocol (GSP) to determine the origin(s) of the Lao DENV-2 and study their genetic polymorphism. With a good correlation with full envelope gene sequencing data, this molecular epidemiology tool evidence the co-circulation of two highly polymorphic DENV-2 genotypes, i.e. Asian I and Cosmopolitan genotypes, over the last five years, suggesting multiple introductions of DENV-2 in the country. GSP approach provides relevant first line information that may help countries with limited laboratory resources to reinforce their capabilities to DENV-2 and to follow the epidemics progresses and assess situations at the regional level.https://doi.org/10.1371/journal.pone.0237384 |
| spellingShingle | Elodie Calvez Somphavanh Somlor Souksakhone Viengphouthong Charlotte Balière Phaithong Bounmany Sitsana Keosenhom Valérie Caro Marc Grandadam Rapid genotyping protocol to improve dengue virus serotype 2 survey in Lao PDR. PLoS ONE |
| title | Rapid genotyping protocol to improve dengue virus serotype 2 survey in Lao PDR. |
| title_full | Rapid genotyping protocol to improve dengue virus serotype 2 survey in Lao PDR. |
| title_fullStr | Rapid genotyping protocol to improve dengue virus serotype 2 survey in Lao PDR. |
| title_full_unstemmed | Rapid genotyping protocol to improve dengue virus serotype 2 survey in Lao PDR. |
| title_short | Rapid genotyping protocol to improve dengue virus serotype 2 survey in Lao PDR. |
| title_sort | rapid genotyping protocol to improve dengue virus serotype 2 survey in lao pdr |
| url | https://doi.org/10.1371/journal.pone.0237384 |
| work_keys_str_mv | AT elodiecalvez rapidgenotypingprotocoltoimprovedenguevirusserotype2surveyinlaopdr AT somphavanhsomlor rapidgenotypingprotocoltoimprovedenguevirusserotype2surveyinlaopdr AT souksakhoneviengphouthong rapidgenotypingprotocoltoimprovedenguevirusserotype2surveyinlaopdr AT charlottebaliere rapidgenotypingprotocoltoimprovedenguevirusserotype2surveyinlaopdr AT phaithongbounmany rapidgenotypingprotocoltoimprovedenguevirusserotype2surveyinlaopdr AT sitsanakeosenhom rapidgenotypingprotocoltoimprovedenguevirusserotype2surveyinlaopdr AT valeriecaro rapidgenotypingprotocoltoimprovedenguevirusserotype2surveyinlaopdr AT marcgrandadam rapidgenotypingprotocoltoimprovedenguevirusserotype2surveyinlaopdr |