Microbial Valorization of Lignin to Bioplastic by Genome-Reduced Pseudomonas putida
As the most abundant natural aromatic resource, lignin valorization will contribute to a feasible biobased economy. Recently, biological lignin valorization has been advocated since ligninolytic microbes possess proficient funneling pathways of lignin to valuable products. In the present study, the...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-05-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.923664/full |
| _version_ | 1811254164496318464 |
|---|---|
| author | Qiu-Jin Zong Tao Xu He Liu Li Xu Ren-Kuan Zhang Bing-Zhi Li Zhi-Hua Liu Ying-Jin Yuan |
| author_facet | Qiu-Jin Zong Tao Xu He Liu Li Xu Ren-Kuan Zhang Bing-Zhi Li Zhi-Hua Liu Ying-Jin Yuan |
| author_sort | Qiu-Jin Zong |
| collection | DOAJ |
| description | As the most abundant natural aromatic resource, lignin valorization will contribute to a feasible biobased economy. Recently, biological lignin valorization has been advocated since ligninolytic microbes possess proficient funneling pathways of lignin to valuable products. In the present study, the potential to convert an actual lignin stream into polyhydroxyalkanoates (PHAs) had been evaluated using ligninolytic genome-reduced Pseudomonas putida. The results showed that the genome-reduced P. putida can grow well on an actual lignin stream to successfully yield a high PHA content and titer. The designed fermentation strategy almost eliminated the substrate effects of lignin on PHA accumulation. Employing a fed-batch strategy produced the comparable PHA contents and titers of 0.35 g/g dried cells and 1.4 g/L, respectively. The molecular mechanism analysis unveiled that P. putida consumed more small and hydrophilic lignin molecules to stimulate cell growth and PHA accumulation. Overall, the genome-reduced P. putida exhibited a superior capacity of lignin bioconversion and promote PHA accumulation, providing a promising route for sustainable lignin valorization. |
| first_indexed | 2024-04-12T17:03:07Z |
| format | Article |
| id | doaj.art-219981f31323443b9bec3a36e32cc455 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T17:03:07Z |
| publishDate | 2022-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-219981f31323443b9bec3a36e32cc4552022-12-22T03:24:01ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-05-011310.3389/fmicb.2022.923664923664Microbial Valorization of Lignin to Bioplastic by Genome-Reduced Pseudomonas putidaQiu-Jin ZongTao XuHe LiuLi XuRen-Kuan ZhangBing-Zhi LiZhi-Hua LiuYing-Jin YuanAs the most abundant natural aromatic resource, lignin valorization will contribute to a feasible biobased economy. Recently, biological lignin valorization has been advocated since ligninolytic microbes possess proficient funneling pathways of lignin to valuable products. In the present study, the potential to convert an actual lignin stream into polyhydroxyalkanoates (PHAs) had been evaluated using ligninolytic genome-reduced Pseudomonas putida. The results showed that the genome-reduced P. putida can grow well on an actual lignin stream to successfully yield a high PHA content and titer. The designed fermentation strategy almost eliminated the substrate effects of lignin on PHA accumulation. Employing a fed-batch strategy produced the comparable PHA contents and titers of 0.35 g/g dried cells and 1.4 g/L, respectively. The molecular mechanism analysis unveiled that P. putida consumed more small and hydrophilic lignin molecules to stimulate cell growth and PHA accumulation. Overall, the genome-reduced P. putida exhibited a superior capacity of lignin bioconversion and promote PHA accumulation, providing a promising route for sustainable lignin valorization.https://www.frontiersin.org/articles/10.3389/fmicb.2022.923664/fullbiological lignin valorizationfed-batch strategyalkaline pretreated liquorpolyhydroxyalkanoateslignin characterization |
| spellingShingle | Qiu-Jin Zong Tao Xu He Liu Li Xu Ren-Kuan Zhang Bing-Zhi Li Zhi-Hua Liu Ying-Jin Yuan Microbial Valorization of Lignin to Bioplastic by Genome-Reduced Pseudomonas putida Frontiers in Microbiology biological lignin valorization fed-batch strategy alkaline pretreated liquor polyhydroxyalkanoates lignin characterization |
| title | Microbial Valorization of Lignin to Bioplastic by Genome-Reduced Pseudomonas putida |
| title_full | Microbial Valorization of Lignin to Bioplastic by Genome-Reduced Pseudomonas putida |
| title_fullStr | Microbial Valorization of Lignin to Bioplastic by Genome-Reduced Pseudomonas putida |
| title_full_unstemmed | Microbial Valorization of Lignin to Bioplastic by Genome-Reduced Pseudomonas putida |
| title_short | Microbial Valorization of Lignin to Bioplastic by Genome-Reduced Pseudomonas putida |
| title_sort | microbial valorization of lignin to bioplastic by genome reduced pseudomonas putida |
| topic | biological lignin valorization fed-batch strategy alkaline pretreated liquor polyhydroxyalkanoates lignin characterization |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.923664/full |
| work_keys_str_mv | AT qiujinzong microbialvalorizationoflignintobioplasticbygenomereducedpseudomonasputida AT taoxu microbialvalorizationoflignintobioplasticbygenomereducedpseudomonasputida AT heliu microbialvalorizationoflignintobioplasticbygenomereducedpseudomonasputida AT lixu microbialvalorizationoflignintobioplasticbygenomereducedpseudomonasputida AT renkuanzhang microbialvalorizationoflignintobioplasticbygenomereducedpseudomonasputida AT bingzhili microbialvalorizationoflignintobioplasticbygenomereducedpseudomonasputida AT zhihualiu microbialvalorizationoflignintobioplasticbygenomereducedpseudomonasputida AT yingjinyuan microbialvalorizationoflignintobioplasticbygenomereducedpseudomonasputida |