Gene-gene interactions of cytokines and C-reactive protein in psoriasis in residents of Kemerovo region
Psoriasis is the most common inflammatory skin disease, affecting on average 2-4% of the world’s population. Currently, psoriasis is considered a multifactorial disease occurring in genetically predisposed individuals under the influence of various environmental factors that trigger a disrupted immu...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | Russian |
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St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists
2025-01-01
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| Series: | Медицинская иммунология |
| Subjects: | |
| Online Access: | https://www.mimmun.ru/mimmun/article/view/2937 |
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| author | E. L. Romanova A. V. Shabaldin E. G. Belov L. V. Striga A. A. Yakovleva E. A. Shevchenko E. V. Shabaldina |
| author_facet | E. L. Romanova A. V. Shabaldin E. G. Belov L. V. Striga A. A. Yakovleva E. A. Shevchenko E. V. Shabaldina |
| author_sort | E. L. Romanova |
| collection | DOAJ |
| description | Psoriasis is the most common inflammatory skin disease, affecting on average 2-4% of the world’s population. Currently, psoriasis is considered a multifactorial disease occurring in genetically predisposed individuals under the influence of various environmental factors that trigger a disrupted immune response via complex inflammatory cascades. The disease is initiated and maintained by the mutual interaction of cells of innate and adaptive immunity, primarily, dendritic cells, T lymphocytes and keratinocytes. Their leading role may alternate at different stages of the disease and proceeds, mainly, at the IL-23/Th17 pathway. To date, many gene polymorphisms (SNPs) associated with the development of psoriasis have been described. To understand the pathophysiology of psoriasis as a complex autoinflammatory disease, it seems interesting to study the intergenic interactions between polymorphic gene variants of cytokines and C-reactive protein related to the risk of psoriasis development. The aim of our study was to investigate intergenic interactions of polymorphic variants of IL1β (rs16944), IL6 (rs1554606), IL8 (rs2227306), IL10 (rs1800896), TNFα (rs361525), CRP (rs1205) genes associated with altered risk of psoriasis development among the Kemerovo Region residents. We examined 175 patients with ordinary papular plaque psoriasis of moderate severity, with progressive course of the disorder. The control group (n = 155) was recruited from conditionally healthy, age-matched donors. Genotyping was performed by PCR using TaqMan probes (Thermo Fisher Scientific, USA), by means of the detection amplifier ViiATM 7 Real-Time PCR System (Life Technologies, USA), for the following polymorphic variants of genes: IL1β (rs16944), IL6 (rs1554606), IL8 (rs2227306), IL10 (rs1800896), TNFα (rs361525), CRP (rs1205). Intergenic interactions were analyzed using Multifactor Dimensionality Reduction (MDR). The common papulosis-plaque psoriasis was found to be associated with individual polymorphic variants of cytokine and CRP genes as well as with gene-gene interactions. Concerning individual polymorphic gene variants, the association strength (% entropy) with common papulosis-plaque psoriasis was as follows: TNFα_ rs361525 (16.99% entropy), IL1β_rs16944 (6.40% entropy), CRP_rs1205 (2.55% entropy), IL6_rs1554606 (1.11% entropy), IL10_rs1800896 (0.57% entropy), IL8_rs2227306 (0.30% entropy). We have found that IL6 (rs1554606) and IL8 (rs2227306) showed marked synergism, as well as moderate synergism of CRP (rs1205) and IL10 (rs1800896), and pronounced antagonism of IL1β (rs16944) and TNFα (rs361525). |
| first_indexed | 2024-04-24T06:48:29Z |
| format | Article |
| id | doaj.art-219acf64850b4c81b8f61b4b84424930 |
| institution | Directory Open Access Journal |
| issn | 1563-0625 2313-741X |
| language | Russian |
| last_indexed | 2025-03-14T02:15:58Z |
| publishDate | 2025-01-01 |
| publisher | St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists |
| record_format | Article |
| series | Медицинская иммунология |
| spelling | doaj.art-219acf64850b4c81b8f61b4b844249302025-03-11T17:59:17ZrusSt. Petersburg branch of the Russian Association of Allergologists and Clinical ImmunologistsМедицинская иммунология1563-06252313-741X2025-01-01271879610.15789/1563-0625-GGI-29371874Gene-gene interactions of cytokines and C-reactive protein in psoriasis in residents of Kemerovo regionE. L. Romanova0A. V. Shabaldin1E. G. Belov2L. V. Striga3A. A. Yakovleva4E. A. Shevchenko5E. V. Shabaldina6Kemerovo State UniversityKemerovo State UniversityKuzbass Clinical Skin and Venereological DispensaryKuzbass Clinical Skin and Venereological DispensaryKemerovo State UniversityKuzbass Clinical Skin and Venereological DispensaryKemerovo State Medical UniversityPsoriasis is the most common inflammatory skin disease, affecting on average 2-4% of the world’s population. Currently, psoriasis is considered a multifactorial disease occurring in genetically predisposed individuals under the influence of various environmental factors that trigger a disrupted immune response via complex inflammatory cascades. The disease is initiated and maintained by the mutual interaction of cells of innate and adaptive immunity, primarily, dendritic cells, T lymphocytes and keratinocytes. Their leading role may alternate at different stages of the disease and proceeds, mainly, at the IL-23/Th17 pathway. To date, many gene polymorphisms (SNPs) associated with the development of psoriasis have been described. To understand the pathophysiology of psoriasis as a complex autoinflammatory disease, it seems interesting to study the intergenic interactions between polymorphic gene variants of cytokines and C-reactive protein related to the risk of psoriasis development. The aim of our study was to investigate intergenic interactions of polymorphic variants of IL1β (rs16944), IL6 (rs1554606), IL8 (rs2227306), IL10 (rs1800896), TNFα (rs361525), CRP (rs1205) genes associated with altered risk of psoriasis development among the Kemerovo Region residents. We examined 175 patients with ordinary papular plaque psoriasis of moderate severity, with progressive course of the disorder. The control group (n = 155) was recruited from conditionally healthy, age-matched donors. Genotyping was performed by PCR using TaqMan probes (Thermo Fisher Scientific, USA), by means of the detection amplifier ViiATM 7 Real-Time PCR System (Life Technologies, USA), for the following polymorphic variants of genes: IL1β (rs16944), IL6 (rs1554606), IL8 (rs2227306), IL10 (rs1800896), TNFα (rs361525), CRP (rs1205). Intergenic interactions were analyzed using Multifactor Dimensionality Reduction (MDR). The common papulosis-plaque psoriasis was found to be associated with individual polymorphic variants of cytokine and CRP genes as well as with gene-gene interactions. Concerning individual polymorphic gene variants, the association strength (% entropy) with common papulosis-plaque psoriasis was as follows: TNFα_ rs361525 (16.99% entropy), IL1β_rs16944 (6.40% entropy), CRP_rs1205 (2.55% entropy), IL6_rs1554606 (1.11% entropy), IL10_rs1800896 (0.57% entropy), IL8_rs2227306 (0.30% entropy). We have found that IL6 (rs1554606) and IL8 (rs2227306) showed marked synergism, as well as moderate synergism of CRP (rs1205) and IL10 (rs1800896), and pronounced antagonism of IL1β (rs16944) and TNFα (rs361525).https://www.mimmun.ru/mimmun/article/view/2937psoriasisil1β (rs16944)il6 (rs1554606)il8 (rs2227306)il10 (rs1800896)tnfα (rs361525)crp (rs1205)multifactor dimensionality reduction |
| spellingShingle | E. L. Romanova A. V. Shabaldin E. G. Belov L. V. Striga A. A. Yakovleva E. A. Shevchenko E. V. Shabaldina Gene-gene interactions of cytokines and C-reactive protein in psoriasis in residents of Kemerovo region Медицинская иммунология psoriasis il1β (rs16944) il6 (rs1554606) il8 (rs2227306) il10 (rs1800896) tnfα (rs361525) crp (rs1205) multifactor dimensionality reduction |
| title | Gene-gene interactions of cytokines and C-reactive protein in psoriasis in residents of Kemerovo region |
| title_full | Gene-gene interactions of cytokines and C-reactive protein in psoriasis in residents of Kemerovo region |
| title_fullStr | Gene-gene interactions of cytokines and C-reactive protein in psoriasis in residents of Kemerovo region |
| title_full_unstemmed | Gene-gene interactions of cytokines and C-reactive protein in psoriasis in residents of Kemerovo region |
| title_short | Gene-gene interactions of cytokines and C-reactive protein in psoriasis in residents of Kemerovo region |
| title_sort | gene gene interactions of cytokines and c reactive protein in psoriasis in residents of kemerovo region |
| topic | psoriasis il1β (rs16944) il6 (rs1554606) il8 (rs2227306) il10 (rs1800896) tnfα (rs361525) crp (rs1205) multifactor dimensionality reduction |
| url | https://www.mimmun.ru/mimmun/article/view/2937 |
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