ГЕН-ГЕННЫЕ ВЗАМОДЕЙСТВИЯ ЦИТОКИНОВ И С-РЕАКТИВНОГО БЕЛКА ПРИ ПСОРИАЗЕ У ЖИТЕЛЕЙ КЕМЕРОВСКОЙ ОБЛАСТИ
Psoriasis is the most common inflammatory skin disease, affecting on average 2-4% of the world's population. According to current understanding, psoriasis is a multifactorial disease occurring in genetically predisposed individuals under the influence of various environmental factors that trigg...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | Russian |
| Published: |
St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists
2019-08-01
|
| Series: | Медицинская иммунология |
| Subjects: | |
| Online Access: | https://www.mimmun.ru/mimmun/article/view/2937 |
| _version_ | 1797197722832338944 |
|---|---|
| author | Елизавета Romanova Леонидовна Andrey Vladimirovich Shabaldin Anastasia Alexandrovna Yakovleva Evgeny Georgievic Belov Larisa Vladimirovna Striga Elena Aleksandrovna Shevchenko Elena Viktorovna Shabaldina |
| author_facet | Елизавета Romanova Леонидовна Andrey Vladimirovich Shabaldin Anastasia Alexandrovna Yakovleva Evgeny Georgievic Belov Larisa Vladimirovna Striga Elena Aleksandrovna Shevchenko Elena Viktorovna Shabaldina |
| author_sort | Елизавета Romanova Леонидовна |
| collection | DOAJ |
| description | Psoriasis is the most common inflammatory skin disease, affecting on average 2-4% of the world's population. According to current understanding, psoriasis is a multifactorial disease occurring in genetically predisposed individuals under the influence of various environmental factors that trigger a disrupted immune response with a series of complex inflammatory cascades. The disease is initiated and maintained by the mutual interaction of cells of innate and adaptive immunity, primarily dendritic cells, T lymphocytes and keratinocytes, whose leading role alternates at different stages of the disease and consists mainly of the IL-23/Th17 pathway. To date, many gene polymorphisms (SNPs) associated with the development of psoriasis have been described. To understand the pathophysiology of psoriasis as a complex autoinflammatory disease it seems interesting to study the intergenic interactions of polymorphic variants of cytokine and C-reactive protein genes in relation to the risk of psoriasis development. The aim of the study was to investigate intergenic interactions of polymorphic variants of IL1b (rs16944), IL6 (rs1554606), IL8 (rs2227306), IL10 (rs1800896), TNFα (rs361525), CRP (rs1205) genes associated with the risk of psoriasis development in Kemerovo region residents. We examined 175 patients with ordinary papular plaque psoriasis, with progressive course, in moderate severity. The control group (n=155) was formed from conditionally healthy donors of the same age interval. Genotyping was performed by PCR using TaqMan probes (Thermo Fisher Scientific, USA) on the detection amplifier ViiATM 7 Real-Time PCR System (Life Technologies, USA) of the following polymorphic variants of genes: IL1b (rs16944), IL6 (rs1554606), IL8 (rs2227306), IL10 (rs1800896), TNFα (rs361525), CRP (rs1205). Intergenic interactions were analyzed using Multifactor Dimensionality Reduction (MDR). It was found that the determination of common papulosis-plaque psoriasis is associated with both individual polymorphic variants of cytokine and CRP genes and gene-gene interactions. For individual polymorphic gene variants, the strength (% entropy) of association with common papulosis-plaque psoriasis was as follows: TNFa_rs361525 (16.99% entropy), IL1b_rs16944 (6.40% entropy), CRP_rs1205 (2.55% entropy), IL6_rs1554606 (1.11% entropy), IL10_rs1800896 (0.57% entropy), IL8_rs2227306 (0.30% entropy). We found that IL6 (rs1554606) and IL8 (rs2227306) had marked synergism; moderate synergism of CRP (rs1205) and IL10 (rs1800896) and marked antagonism of IL1b (rs16944) and TNFa (rs361525). |
| first_indexed | 2024-04-24T06:48:29Z |
| format | Article |
| id | doaj.art-219acf64850b4c81b8f61b4b84424930 |
| institution | Directory Open Access Journal |
| issn | 1563-0625 2313-741X |
| language | Russian |
| last_indexed | 2024-04-24T06:48:29Z |
| publishDate | 2019-08-01 |
| publisher | St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists |
| record_format | Article |
| series | Медицинская иммунология |
| spelling | doaj.art-219acf64850b4c81b8f61b4b844249302024-04-22T13:07:52ZrusSt. Petersburg branch of the Russian Association of Allergologists and Clinical ImmunologistsМедицинская иммунология1563-06252313-741X2019-08-010010.15789/1563-0625-GIO-29371874ГЕН-ГЕННЫЕ ВЗАМОДЕЙСТВИЯ ЦИТОКИНОВ И С-РЕАКТИВНОГО БЕЛКА ПРИ ПСОРИАЗЕ У ЖИТЕЛЕЙ КЕМЕРОВСКОЙ ОБЛАСТИЕлизавета Romanova Леонидовна0Andrey Vladimirovich Shabaldin1Anastasia Alexandrovna Yakovleva2Evgeny Georgievic Belov3Larisa Vladimirovna Striga4Elena Aleksandrovna Shevchenko5Elena Viktorovna Shabaldina6Kemerovo State UniversityKemerovo State UniversityKemerovo State Universityof the State Budgetary Institution "Kuzbass Clinical Skin and Venereological Dispensary", Kemerovo, RussiaState Budgetary Institution “Kuzbass Clinical Skin and Venereological Dispensary”, Kemerovo, Russia State Budgetary Institution "Kuzbass Clinical Skin and Venereological Dispensary", Kemerovo, RussiaKemerovo State Medical University, Ministry of Health of RussiaPsoriasis is the most common inflammatory skin disease, affecting on average 2-4% of the world's population. According to current understanding, psoriasis is a multifactorial disease occurring in genetically predisposed individuals under the influence of various environmental factors that trigger a disrupted immune response with a series of complex inflammatory cascades. The disease is initiated and maintained by the mutual interaction of cells of innate and adaptive immunity, primarily dendritic cells, T lymphocytes and keratinocytes, whose leading role alternates at different stages of the disease and consists mainly of the IL-23/Th17 pathway. To date, many gene polymorphisms (SNPs) associated with the development of psoriasis have been described. To understand the pathophysiology of psoriasis as a complex autoinflammatory disease it seems interesting to study the intergenic interactions of polymorphic variants of cytokine and C-reactive protein genes in relation to the risk of psoriasis development. The aim of the study was to investigate intergenic interactions of polymorphic variants of IL1b (rs16944), IL6 (rs1554606), IL8 (rs2227306), IL10 (rs1800896), TNFα (rs361525), CRP (rs1205) genes associated with the risk of psoriasis development in Kemerovo region residents. We examined 175 patients with ordinary papular plaque psoriasis, with progressive course, in moderate severity. The control group (n=155) was formed from conditionally healthy donors of the same age interval. Genotyping was performed by PCR using TaqMan probes (Thermo Fisher Scientific, USA) on the detection amplifier ViiATM 7 Real-Time PCR System (Life Technologies, USA) of the following polymorphic variants of genes: IL1b (rs16944), IL6 (rs1554606), IL8 (rs2227306), IL10 (rs1800896), TNFα (rs361525), CRP (rs1205). Intergenic interactions were analyzed using Multifactor Dimensionality Reduction (MDR). It was found that the determination of common papulosis-plaque psoriasis is associated with both individual polymorphic variants of cytokine and CRP genes and gene-gene interactions. For individual polymorphic gene variants, the strength (% entropy) of association with common papulosis-plaque psoriasis was as follows: TNFa_rs361525 (16.99% entropy), IL1b_rs16944 (6.40% entropy), CRP_rs1205 (2.55% entropy), IL6_rs1554606 (1.11% entropy), IL10_rs1800896 (0.57% entropy), IL8_rs2227306 (0.30% entropy). We found that IL6 (rs1554606) and IL8 (rs2227306) had marked synergism; moderate synergism of CRP (rs1205) and IL10 (rs1800896) and marked antagonism of IL1b (rs16944) and TNFa (rs361525).https://www.mimmun.ru/mimmun/article/view/2937psoriasisil1b (rs16944)il6 (rs1554606)il8 (rs2227306)il10 (rs1800896)tnfα (rs361525)crp (rs1205)multifactor dimensionality reduction (mdr). |
| spellingShingle | Елизавета Romanova Леонидовна Andrey Vladimirovich Shabaldin Anastasia Alexandrovna Yakovleva Evgeny Georgievic Belov Larisa Vladimirovna Striga Elena Aleksandrovna Shevchenko Elena Viktorovna Shabaldina ГЕН-ГЕННЫЕ ВЗАМОДЕЙСТВИЯ ЦИТОКИНОВ И С-РЕАКТИВНОГО БЕЛКА ПРИ ПСОРИАЗЕ У ЖИТЕЛЕЙ КЕМЕРОВСКОЙ ОБЛАСТИ Медицинская иммунология psoriasis il1b (rs16944) il6 (rs1554606) il8 (rs2227306) il10 (rs1800896) tnfα (rs361525) crp (rs1205) multifactor dimensionality reduction (mdr). |
| title | ГЕН-ГЕННЫЕ ВЗАМОДЕЙСТВИЯ ЦИТОКИНОВ И С-РЕАКТИВНОГО БЕЛКА ПРИ ПСОРИАЗЕ У ЖИТЕЛЕЙ КЕМЕРОВСКОЙ ОБЛАСТИ |
| title_full | ГЕН-ГЕННЫЕ ВЗАМОДЕЙСТВИЯ ЦИТОКИНОВ И С-РЕАКТИВНОГО БЕЛКА ПРИ ПСОРИАЗЕ У ЖИТЕЛЕЙ КЕМЕРОВСКОЙ ОБЛАСТИ |
| title_fullStr | ГЕН-ГЕННЫЕ ВЗАМОДЕЙСТВИЯ ЦИТОКИНОВ И С-РЕАКТИВНОГО БЕЛКА ПРИ ПСОРИАЗЕ У ЖИТЕЛЕЙ КЕМЕРОВСКОЙ ОБЛАСТИ |
| title_full_unstemmed | ГЕН-ГЕННЫЕ ВЗАМОДЕЙСТВИЯ ЦИТОКИНОВ И С-РЕАКТИВНОГО БЕЛКА ПРИ ПСОРИАЗЕ У ЖИТЕЛЕЙ КЕМЕРОВСКОЙ ОБЛАСТИ |
| title_short | ГЕН-ГЕННЫЕ ВЗАМОДЕЙСТВИЯ ЦИТОКИНОВ И С-РЕАКТИВНОГО БЕЛКА ПРИ ПСОРИАЗЕ У ЖИТЕЛЕЙ КЕМЕРОВСКОЙ ОБЛАСТИ |
| title_sort | ген генные взамодействия цитокинов и с реактивного белка при псориазе у жителей кемеровской области |
| topic | psoriasis il1b (rs16944) il6 (rs1554606) il8 (rs2227306) il10 (rs1800896) tnfα (rs361525) crp (rs1205) multifactor dimensionality reduction (mdr). |
| url | https://www.mimmun.ru/mimmun/article/view/2937 |
| work_keys_str_mv | AT elizavetaromanovaleonidovna gengennyevzamodejstviâcitokinovisreaktivnogobelkapripsoriazeužitelejkemerovskojoblasti AT andreyvladimirovichshabaldin gengennyevzamodejstviâcitokinovisreaktivnogobelkapripsoriazeužitelejkemerovskojoblasti AT anastasiaalexandrovnayakovleva gengennyevzamodejstviâcitokinovisreaktivnogobelkapripsoriazeužitelejkemerovskojoblasti AT evgenygeorgievicbelov gengennyevzamodejstviâcitokinovisreaktivnogobelkapripsoriazeužitelejkemerovskojoblasti AT larisavladimirovnastriga gengennyevzamodejstviâcitokinovisreaktivnogobelkapripsoriazeužitelejkemerovskojoblasti AT elenaaleksandrovnashevchenko gengennyevzamodejstviâcitokinovisreaktivnogobelkapripsoriazeužitelejkemerovskojoblasti AT elenaviktorovnashabaldina gengennyevzamodejstviâcitokinovisreaktivnogobelkapripsoriazeužitelejkemerovskojoblasti |