A cross-sectional study of clinical, histopathological and direct immmunofluorescence diagnosis in autoimmune bullous diseases
Background: Immunobullous diseases are morphologically heterogeneous and the differentiation between various subtypes is essential for proper treatment and prognosis. Aim of our study was to analyze and correlate clinical, histopathological and immunofluorescence findings in autoimmune bullous disea...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2014-01-01
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| Series: | Indian Journal of Dermatopathology and Diagnostic Dermatology |
| Subjects: | |
| Online Access: | http://www.ijdpdd.com/article.asp?issn=2349-6029;year=2014;volume=1;issue=1;spage=25;epage=31;aulast=Jindal |
| _version_ | 1818889496559616000 |
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| author | Anchal Jindal Rushikesh Shah Neela Patel Krina Patel Rupal P Mehta Jigna P Barot |
| author_facet | Anchal Jindal Rushikesh Shah Neela Patel Krina Patel Rupal P Mehta Jigna P Barot |
| author_sort | Anchal Jindal |
| collection | DOAJ |
| description | Background: Immunobullous diseases are morphologically heterogeneous and the differentiation between various subtypes is essential for proper treatment and prognosis. Aim of our study was to analyze and correlate clinical, histopathological and immunofluorescence findings in autoimmune bullous diseases. Materials and Methods: A cross-sectional study was done over a period of two years (2010-2012) after approval of the ethics committee. Sixty patients, who met the inclusion criteria of immunobullous disease, were included in the study. Skin biopsy for histopathology and direct immunofluorescence (DIF) examination was taken. DIF using salt-split technique was done in few of the cases. The final diagnosis was based on clinical, histopathology and DIF findings. Pearson′s coefficient of correlation (r) was calculated. Statistical Analysis was done using Epi info version. 7.0. Results: Fifty-three cases with clinical diagnosis of autoimmune bullous diseases were evaluated. In 88.6% of cases, histopathology diagnosis was consistent with clinical diagnosis and in 75.5% of cases, DIF findings were consistent with clinical diagnosis. A positive relation was seen between clinical and DIF findings with r = 0.65 and between histopathology and DIF findings with r = 0.75. DIF positivity was seen in 100% cases of bullous pemphigoid (BP) and pemphigus foliaceous and 94.7% cases of pemphigus vulgaris, which was statistically significant with p < 0.05. In DIF salt-split test, deposition was seen on roof of blister in BP whereas on floor in epidermolysis bullosa acquisita. Conclusion: Our study provides evidence-based guidance for the diagnosis and classification of various immunobullous disorders. DIF test should be done in conjunction with histopathology for definitive diagnosis and to minimize both: False-positive and false-negative results. |
| first_indexed | 2024-12-19T17:09:57Z |
| format | Article |
| id | doaj.art-219fa5863592431595e7f2dcd854a1f1 |
| institution | Directory Open Access Journal |
| issn | 2349-6029 2349-6029 |
| language | English |
| last_indexed | 2024-12-19T17:09:57Z |
| publishDate | 2014-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Indian Journal of Dermatopathology and Diagnostic Dermatology |
| spelling | doaj.art-219fa5863592431595e7f2dcd854a1f12022-12-21T20:13:03ZengWolters Kluwer Medknow PublicationsIndian Journal of Dermatopathology and Diagnostic Dermatology2349-60292349-60292014-01-01112531A cross-sectional study of clinical, histopathological and direct immmunofluorescence diagnosis in autoimmune bullous diseasesAnchal JindalRushikesh ShahNeela PatelKrina PatelRupal P MehtaJigna P BarotBackground: Immunobullous diseases are morphologically heterogeneous and the differentiation between various subtypes is essential for proper treatment and prognosis. Aim of our study was to analyze and correlate clinical, histopathological and immunofluorescence findings in autoimmune bullous diseases. Materials and Methods: A cross-sectional study was done over a period of two years (2010-2012) after approval of the ethics committee. Sixty patients, who met the inclusion criteria of immunobullous disease, were included in the study. Skin biopsy for histopathology and direct immunofluorescence (DIF) examination was taken. DIF using salt-split technique was done in few of the cases. The final diagnosis was based on clinical, histopathology and DIF findings. Pearson′s coefficient of correlation (r) was calculated. Statistical Analysis was done using Epi info version. 7.0. Results: Fifty-three cases with clinical diagnosis of autoimmune bullous diseases were evaluated. In 88.6% of cases, histopathology diagnosis was consistent with clinical diagnosis and in 75.5% of cases, DIF findings were consistent with clinical diagnosis. A positive relation was seen between clinical and DIF findings with r = 0.65 and between histopathology and DIF findings with r = 0.75. DIF positivity was seen in 100% cases of bullous pemphigoid (BP) and pemphigus foliaceous and 94.7% cases of pemphigus vulgaris, which was statistically significant with p < 0.05. In DIF salt-split test, deposition was seen on roof of blister in BP whereas on floor in epidermolysis bullosa acquisita. Conclusion: Our study provides evidence-based guidance for the diagnosis and classification of various immunobullous disorders. DIF test should be done in conjunction with histopathology for definitive diagnosis and to minimize both: False-positive and false-negative results.http://www.ijdpdd.com/article.asp?issn=2349-6029;year=2014;volume=1;issue=1;spage=25;epage=31;aulast=JindalHistopathologyimmunobullousimmunofluorescence |
| spellingShingle | Anchal Jindal Rushikesh Shah Neela Patel Krina Patel Rupal P Mehta Jigna P Barot A cross-sectional study of clinical, histopathological and direct immmunofluorescence diagnosis in autoimmune bullous diseases Indian Journal of Dermatopathology and Diagnostic Dermatology Histopathology immunobullous immunofluorescence |
| title | A cross-sectional study of clinical, histopathological and direct immmunofluorescence diagnosis in autoimmune bullous diseases |
| title_full | A cross-sectional study of clinical, histopathological and direct immmunofluorescence diagnosis in autoimmune bullous diseases |
| title_fullStr | A cross-sectional study of clinical, histopathological and direct immmunofluorescence diagnosis in autoimmune bullous diseases |
| title_full_unstemmed | A cross-sectional study of clinical, histopathological and direct immmunofluorescence diagnosis in autoimmune bullous diseases |
| title_short | A cross-sectional study of clinical, histopathological and direct immmunofluorescence diagnosis in autoimmune bullous diseases |
| title_sort | cross sectional study of clinical histopathological and direct immmunofluorescence diagnosis in autoimmune bullous diseases |
| topic | Histopathology immunobullous immunofluorescence |
| url | http://www.ijdpdd.com/article.asp?issn=2349-6029;year=2014;volume=1;issue=1;spage=25;epage=31;aulast=Jindal |
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