Biomolecular condensate phase diagrams with a combinatorial microdroplet platform
A central concept for characterising phase-separating systems is the phase diagram but generation of such diagrams for biomolecular systems is typically slow and low-throughput. Here the authors describe PhaseScan, a combinatorial droplet microfluidic platform for high-resolution acquisition of mult...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-12-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-022-35265-7 |
| _version_ | 1797977321821437952 |
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| author | William E. Arter Runzhang Qi Nadia A. Erkamp Georg Krainer Kieran Didi Timothy J. Welsh Julia Acker Jonathan Nixon-Abell Seema Qamar Jordina Guillén-Boixet Titus M. Franzmann David Kuster Anthony A. Hyman Alexander Borodavka Peter St George-Hyslop Simon Alberti Tuomas P. J. Knowles |
| author_facet | William E. Arter Runzhang Qi Nadia A. Erkamp Georg Krainer Kieran Didi Timothy J. Welsh Julia Acker Jonathan Nixon-Abell Seema Qamar Jordina Guillén-Boixet Titus M. Franzmann David Kuster Anthony A. Hyman Alexander Borodavka Peter St George-Hyslop Simon Alberti Tuomas P. J. Knowles |
| author_sort | William E. Arter |
| collection | DOAJ |
| description | A central concept for characterising phase-separating systems is the phase diagram but generation of such diagrams for biomolecular systems is typically slow and low-throughput. Here the authors describe PhaseScan, a combinatorial droplet microfluidic platform for high-resolution acquisition of multidimensional biomolecular phase diagrams. |
| first_indexed | 2024-04-11T05:04:58Z |
| format | Article |
| id | doaj.art-21a365c2a2af4ba08de716204af10bed |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-04-11T05:04:58Z |
| publishDate | 2022-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-21a365c2a2af4ba08de716204af10bed2022-12-25T12:22:50ZengNature PortfolioNature Communications2041-17232022-12-0113111010.1038/s41467-022-35265-7Biomolecular condensate phase diagrams with a combinatorial microdroplet platformWilliam E. Arter0Runzhang Qi1Nadia A. Erkamp2Georg Krainer3Kieran Didi4Timothy J. Welsh5Julia Acker6Jonathan Nixon-Abell7Seema Qamar8Jordina Guillén-Boixet9Titus M. Franzmann10David Kuster11Anthony A. Hyman12Alexander Borodavka13Peter St George-Hyslop14Simon Alberti15Tuomas P. J. Knowles16Yusuf Hamied Department of Chemistry, Centre for Misfolding Diseases, University of CambridgeYusuf Hamied Department of Chemistry, Centre for Misfolding Diseases, University of CambridgeYusuf Hamied Department of Chemistry, Centre for Misfolding Diseases, University of CambridgeYusuf Hamied Department of Chemistry, Centre for Misfolding Diseases, University of CambridgeYusuf Hamied Department of Chemistry, Centre for Misfolding Diseases, University of CambridgeYusuf Hamied Department of Chemistry, Centre for Misfolding Diseases, University of CambridgeDepartment of Biochemistry, University of CambridgeCambridge Institute for Medical Research, Department of Clinical Neurosciences, University of CambridgeCambridge Institute for Medical Research, Department of Clinical Neurosciences, University of CambridgeBiotechnology Center (BIOTEC), Center for Molecular and Cellular Bioengineering (CMCB), Technische Universität DresdenBiotechnology Center (BIOTEC), Center for Molecular and Cellular Bioengineering (CMCB), Technische Universität DresdenMax Planck Institute for Molecular Cell Biology and GeneticsMax Planck Institute for Molecular Cell Biology and GeneticsDepartment of Biochemistry, University of CambridgeCambridge Institute for Medical Research, Department of Clinical Neurosciences, University of CambridgeBiotechnology Center (BIOTEC), Center for Molecular and Cellular Bioengineering (CMCB), Technische Universität DresdenYusuf Hamied Department of Chemistry, Centre for Misfolding Diseases, University of CambridgeA central concept for characterising phase-separating systems is the phase diagram but generation of such diagrams for biomolecular systems is typically slow and low-throughput. Here the authors describe PhaseScan, a combinatorial droplet microfluidic platform for high-resolution acquisition of multidimensional biomolecular phase diagrams.https://doi.org/10.1038/s41467-022-35265-7 |
| spellingShingle | William E. Arter Runzhang Qi Nadia A. Erkamp Georg Krainer Kieran Didi Timothy J. Welsh Julia Acker Jonathan Nixon-Abell Seema Qamar Jordina Guillén-Boixet Titus M. Franzmann David Kuster Anthony A. Hyman Alexander Borodavka Peter St George-Hyslop Simon Alberti Tuomas P. J. Knowles Biomolecular condensate phase diagrams with a combinatorial microdroplet platform Nature Communications |
| title | Biomolecular condensate phase diagrams with a combinatorial microdroplet platform |
| title_full | Biomolecular condensate phase diagrams with a combinatorial microdroplet platform |
| title_fullStr | Biomolecular condensate phase diagrams with a combinatorial microdroplet platform |
| title_full_unstemmed | Biomolecular condensate phase diagrams with a combinatorial microdroplet platform |
| title_short | Biomolecular condensate phase diagrams with a combinatorial microdroplet platform |
| title_sort | biomolecular condensate phase diagrams with a combinatorial microdroplet platform |
| url | https://doi.org/10.1038/s41467-022-35265-7 |
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