Noninvasive Combined Diagnosis and Monitoring of <i>Aspergillus</i> and <i>Pseudomonas</i> Infections: Proof of Concept
In acutely ill patients, particularly in intensive care units or in mixed infections, time to a microbe-specific diagnosis is critical to a successful outcome of therapy. We report the application of evolving technologies involving mass spectrometry to diagnose and monitor a patient’s course. As pro...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-09-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/7/9/730 |
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| author | Radim Dobiáš Anton Škríba Tomáš Pluháček Miloš Petřík Andrea Palyzová Marcela Káňová Eva Čubová Jiří Houšť Jiří Novák David A. Stevens Goran Mitulovič Eva Krejčí Petr Hubáček Vladimír Havlíček |
| author_facet | Radim Dobiáš Anton Škríba Tomáš Pluháček Miloš Petřík Andrea Palyzová Marcela Káňová Eva Čubová Jiří Houšť Jiří Novák David A. Stevens Goran Mitulovič Eva Krejčí Petr Hubáček Vladimír Havlíček |
| author_sort | Radim Dobiáš |
| collection | DOAJ |
| description | In acutely ill patients, particularly in intensive care units or in mixed infections, time to a microbe-specific diagnosis is critical to a successful outcome of therapy. We report the application of evolving technologies involving mass spectrometry to diagnose and monitor a patient’s course. As proof of this concept, we studied five patients and used two rat models of mono-infection and coinfection. We report the noninvasive combined monitoring of <i>Aspergillus fumigatus</i> and <i>Pseudomonas aeruginosa</i> infection. The invasive coinfection was detected by monitoring the fungal triacetylfusarinine C and ferricrocin siderophore levels and the bacterial metabolites pyoverdin E, pyochelin, and 2-heptyl-4-quinolone, studied in the urine, endotracheal aspirate, or breath condensate. The coinfection was monitored by mass spectrometry followed by isotopic data filtering. In the rat infection model, detection indicated 100-fold more siderophores in urine compared to sera, indicating the diagnostic potential of urine sampling. The tools utilized in our studies can now be examined in large clinical series, where we could expect the accuracy and speed of diagnosis to be competitive with conventional methods and provide advantages in unraveling the complexities of mixed infections. |
| first_indexed | 2024-03-10T07:31:38Z |
| format | Article |
| id | doaj.art-21a56f8d0d064de4b407cfd50288e319 |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-10T07:31:38Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-21a56f8d0d064de4b407cfd50288e3192023-11-22T13:48:05ZengMDPI AGJournal of Fungi2309-608X2021-09-017973010.3390/jof7090730Noninvasive Combined Diagnosis and Monitoring of <i>Aspergillus</i> and <i>Pseudomonas</i> Infections: Proof of ConceptRadim Dobiáš0Anton Škríba1Tomáš Pluháček2Miloš Petřík3Andrea Palyzová4Marcela Káňová5Eva Čubová6Jiří Houšť7Jiří Novák8David A. Stevens9Goran Mitulovič10Eva Krejčí11Petr Hubáček12Vladimír Havlíček13Department of Bacteriology and Mycology, Public Health Institute in Ostrava, 702 00 Ostrava, Czech RepublicInstitute of Microbiology of the Czech Academy of Sciences, 142 20 Prague, Czech RepublicInstitute of Microbiology of the Czech Academy of Sciences, 142 20 Prague, Czech RepublicInstitute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University, 779 00 Olomouc, Czech RepublicInstitute of Microbiology of the Czech Academy of Sciences, 142 20 Prague, Czech RepublicDepartment of Anesthesiology and Intensive Care Medicine, University Hospital Ostrava, 708 00 Ostrava, Czech RepublicDepartment of Internal Medicine, Ostrava City Hospital, 728 80 Ostrava, Czech RepublicInstitute of Microbiology of the Czech Academy of Sciences, 142 20 Prague, Czech RepublicInstitute of Microbiology of the Czech Academy of Sciences, 142 20 Prague, Czech RepublicInfectious Disease Research Laboratory, California Institute for Medical Research, San Jose, CA 95128, USAClinical Department of Laboratory Medicine Proteomics Core Facility, Medical University of Vienna, A-1090 Wien, AustriaDepartment of Bacteriology and Mycology, Public Health Institute in Ostrava, 702 00 Ostrava, Czech RepublicDepartment of Medical Microbiology, 2nd Faculty of Medicine, Charles University and Motol University Hospital, 150 06 Prague, Czech RepublicInstitute of Microbiology of the Czech Academy of Sciences, 142 20 Prague, Czech RepublicIn acutely ill patients, particularly in intensive care units or in mixed infections, time to a microbe-specific diagnosis is critical to a successful outcome of therapy. We report the application of evolving technologies involving mass spectrometry to diagnose and monitor a patient’s course. As proof of this concept, we studied five patients and used two rat models of mono-infection and coinfection. We report the noninvasive combined monitoring of <i>Aspergillus fumigatus</i> and <i>Pseudomonas aeruginosa</i> infection. The invasive coinfection was detected by monitoring the fungal triacetylfusarinine C and ferricrocin siderophore levels and the bacterial metabolites pyoverdin E, pyochelin, and 2-heptyl-4-quinolone, studied in the urine, endotracheal aspirate, or breath condensate. The coinfection was monitored by mass spectrometry followed by isotopic data filtering. In the rat infection model, detection indicated 100-fold more siderophores in urine compared to sera, indicating the diagnostic potential of urine sampling. The tools utilized in our studies can now be examined in large clinical series, where we could expect the accuracy and speed of diagnosis to be competitive with conventional methods and provide advantages in unraveling the complexities of mixed infections.https://www.mdpi.com/2309-608X/7/9/730<i>Aspergillus fumigatus</i><i>Pseudomonas aeruginosa</i>invasive infectionnoninvasive diagnosiscoinfectionvirulence factor |
| spellingShingle | Radim Dobiáš Anton Škríba Tomáš Pluháček Miloš Petřík Andrea Palyzová Marcela Káňová Eva Čubová Jiří Houšť Jiří Novák David A. Stevens Goran Mitulovič Eva Krejčí Petr Hubáček Vladimír Havlíček Noninvasive Combined Diagnosis and Monitoring of <i>Aspergillus</i> and <i>Pseudomonas</i> Infections: Proof of Concept Journal of Fungi <i>Aspergillus fumigatus</i> <i>Pseudomonas aeruginosa</i> invasive infection noninvasive diagnosis coinfection virulence factor |
| title | Noninvasive Combined Diagnosis and Monitoring of <i>Aspergillus</i> and <i>Pseudomonas</i> Infections: Proof of Concept |
| title_full | Noninvasive Combined Diagnosis and Monitoring of <i>Aspergillus</i> and <i>Pseudomonas</i> Infections: Proof of Concept |
| title_fullStr | Noninvasive Combined Diagnosis and Monitoring of <i>Aspergillus</i> and <i>Pseudomonas</i> Infections: Proof of Concept |
| title_full_unstemmed | Noninvasive Combined Diagnosis and Monitoring of <i>Aspergillus</i> and <i>Pseudomonas</i> Infections: Proof of Concept |
| title_short | Noninvasive Combined Diagnosis and Monitoring of <i>Aspergillus</i> and <i>Pseudomonas</i> Infections: Proof of Concept |
| title_sort | noninvasive combined diagnosis and monitoring of i aspergillus i and i pseudomonas i infections proof of concept |
| topic | <i>Aspergillus fumigatus</i> <i>Pseudomonas aeruginosa</i> invasive infection noninvasive diagnosis coinfection virulence factor |
| url | https://www.mdpi.com/2309-608X/7/9/730 |
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