Cinnamon extract improves abnormalities in glucose tolerance by decreasing Acyl-CoA synthetase long-chain family 1 expression in adipocytes
Abstract We previously demonstrated that cinnamon extract (CE) alleviates streptozotocin-induced type 1 diabetes in rats. The present study aimed to elucidate the detailed molecular target of cinnamon in cultured adipocytes and epididymal adipose tissue of type 2 diabetes model mice. Two-dimensional...
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| Format: | Article |
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Nature Portfolio
2022-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-13421-9 |
| _version_ | 1811287281165664256 |
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| author | Tsubame Nishikai-Shen Tomomi Hosono-Fukao Toyohiko Ariga Takashi Hosono Taiichiro Seki |
| author_facet | Tsubame Nishikai-Shen Tomomi Hosono-Fukao Toyohiko Ariga Takashi Hosono Taiichiro Seki |
| author_sort | Tsubame Nishikai-Shen |
| collection | DOAJ |
| description | Abstract We previously demonstrated that cinnamon extract (CE) alleviates streptozotocin-induced type 1 diabetes in rats. The present study aimed to elucidate the detailed molecular target of cinnamon in cultured adipocytes and epididymal adipose tissue of type 2 diabetes model mice. Two-dimensional gel electrophoresis was employed to determine the molecular target of cinnamon in adipocytes. The function of Acyl-CoA synthetase long-chain family-1 (ACSL1), a molecular target of cinnamon that was identified in this study, was further investigated in 3T3-L1 adipocytes using specific inhibitors. Type 2 diabetes model mice (KK-Ay/TaJcl) were used to investigate the effect of CE on glucose tolerance, ACSL1 expression, and related signal molecules in vivo. CE decreased ACSL1 mRNA and protein expression in 3T3-L1 adipocytes but increased glucose uptake and AMPK signaling activation; moreover, a similar effect was observed with an ACSL1 inhibitor. CE improved glucose tolerance and downregulated ACSL1 in mice adipose tissue in vivo. ACSL1 was demonstrated as a molecular target of CE in type 2 diabetes both in a cell culture system and diabetic mouse model. |
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| id | doaj.art-21a7982da4b449fd91328a386a67ecef |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-13T03:16:07Z |
| publishDate | 2022-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-21a7982da4b449fd91328a386a67ecef2022-12-22T03:04:55ZengNature PortfolioScientific Reports2045-23222022-07-0112111210.1038/s41598-022-13421-9Cinnamon extract improves abnormalities in glucose tolerance by decreasing Acyl-CoA synthetase long-chain family 1 expression in adipocytesTsubame Nishikai-Shen0Tomomi Hosono-Fukao1Toyohiko Ariga2Takashi Hosono3Taiichiro Seki4Department of Chemistry and Life Science, College of Bioresource Sciences, Nihon UniversityDepartment of Chemistry and Life Science, College of Bioresource Sciences, Nihon UniversityDepartment of Chemistry and Life Science, College of Bioresource Sciences, Nihon UniversityDepartment of Chemistry and Life Science, College of Bioresource Sciences, Nihon UniversityDepartment of Chemistry and Life Science, College of Bioresource Sciences, Nihon UniversityAbstract We previously demonstrated that cinnamon extract (CE) alleviates streptozotocin-induced type 1 diabetes in rats. The present study aimed to elucidate the detailed molecular target of cinnamon in cultured adipocytes and epididymal adipose tissue of type 2 diabetes model mice. Two-dimensional gel electrophoresis was employed to determine the molecular target of cinnamon in adipocytes. The function of Acyl-CoA synthetase long-chain family-1 (ACSL1), a molecular target of cinnamon that was identified in this study, was further investigated in 3T3-L1 adipocytes using specific inhibitors. Type 2 diabetes model mice (KK-Ay/TaJcl) were used to investigate the effect of CE on glucose tolerance, ACSL1 expression, and related signal molecules in vivo. CE decreased ACSL1 mRNA and protein expression in 3T3-L1 adipocytes but increased glucose uptake and AMPK signaling activation; moreover, a similar effect was observed with an ACSL1 inhibitor. CE improved glucose tolerance and downregulated ACSL1 in mice adipose tissue in vivo. ACSL1 was demonstrated as a molecular target of CE in type 2 diabetes both in a cell culture system and diabetic mouse model.https://doi.org/10.1038/s41598-022-13421-9 |
| spellingShingle | Tsubame Nishikai-Shen Tomomi Hosono-Fukao Toyohiko Ariga Takashi Hosono Taiichiro Seki Cinnamon extract improves abnormalities in glucose tolerance by decreasing Acyl-CoA synthetase long-chain family 1 expression in adipocytes Scientific Reports |
| title | Cinnamon extract improves abnormalities in glucose tolerance by decreasing Acyl-CoA synthetase long-chain family 1 expression in adipocytes |
| title_full | Cinnamon extract improves abnormalities in glucose tolerance by decreasing Acyl-CoA synthetase long-chain family 1 expression in adipocytes |
| title_fullStr | Cinnamon extract improves abnormalities in glucose tolerance by decreasing Acyl-CoA synthetase long-chain family 1 expression in adipocytes |
| title_full_unstemmed | Cinnamon extract improves abnormalities in glucose tolerance by decreasing Acyl-CoA synthetase long-chain family 1 expression in adipocytes |
| title_short | Cinnamon extract improves abnormalities in glucose tolerance by decreasing Acyl-CoA synthetase long-chain family 1 expression in adipocytes |
| title_sort | cinnamon extract improves abnormalities in glucose tolerance by decreasing acyl coa synthetase long chain family 1 expression in adipocytes |
| url | https://doi.org/10.1038/s41598-022-13421-9 |
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