Antimicrobial activity of Tulsi (Ocimum tenuiflorum) essential oil and their major constituents against three species of bacteria
In recent years scientists worldwide have realized that the effective life span of any antimicrobial agent is limited, due to increasing development of resistance by microorganisms. Consequently, numerous studies have been conducted to find new alternative sources of antimicrobial agents, especially...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2016-05-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.00681/full |
| _version_ | 1828762711881678848 |
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| author | Hanaa Abbas M Yamani Hanaa Abbas M Yamani EDWIN CHI KYONG PANG Nitin eMantri Margaret Anne Deighton |
| author_facet | Hanaa Abbas M Yamani Hanaa Abbas M Yamani EDWIN CHI KYONG PANG Nitin eMantri Margaret Anne Deighton |
| author_sort | Hanaa Abbas M Yamani |
| collection | DOAJ |
| description | In recent years scientists worldwide have realized that the effective life span of any antimicrobial agent is limited, due to increasing development of resistance by microorganisms. Consequently, numerous studies have been conducted to find new alternative sources of antimicrobial agents, especially from plants. The aims of this project were to examine the antimicrobial properties of essential oils distilled from Australian-grown Ocimum teniflorum (Tulsi), to quantify the volatile components present in flower spikes, leaves and the essential oil, and to investigate the compounds responsible for any activity. Broth micro-dilution was used to determine the minimum inhibitory concentration (MIC) of Tulsi essential oil against selected microbial pathogens. The oils, at concentrations of 4.5% and 2.25% completely inhibited the growth of Staphylococcus aureus (including MRSA) and Escherichia coli, while the same concentrations only partly inhibited the growth of Pseudomonas aeruginosa. Of 54 compounds identified in Tulsi leaves, flower spikes or essential oil, three are proposed to be responsible for this activity; camphor, eucalyptol and eugenol. Since S. aureus (including MRSA), P. aeruginosa and E. coli are major pathogens causing skin and soft tissue infections, Tulsi essential oil could be a valuable topical antimicrobial agent for management of skin infections caused by these organisms. |
| first_indexed | 2024-12-11T01:48:32Z |
| format | Article |
| id | doaj.art-21a88f4a2a714125ad8b57f1a1033048 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-11T01:48:32Z |
| publishDate | 2016-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-21a88f4a2a714125ad8b57f1a10330482022-12-22T01:24:49ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2016-05-01710.3389/fmicb.2016.00681195885Antimicrobial activity of Tulsi (Ocimum tenuiflorum) essential oil and their major constituents against three species of bacteriaHanaa Abbas M Yamani0Hanaa Abbas M Yamani1EDWIN CHI KYONG PANG2Nitin eMantri3Margaret Anne Deighton4Royal Melbourne Institute of Technology (RMIT) UniversityKing Abdulaziz UniversityRoyal Melbourne Institute of Technology (RMIT) UniversityRoyal Melbourne Institute of Technology (RMIT) UniversityRoyal Melbourne Institute of Technology (RMIT) UniversityIn recent years scientists worldwide have realized that the effective life span of any antimicrobial agent is limited, due to increasing development of resistance by microorganisms. Consequently, numerous studies have been conducted to find new alternative sources of antimicrobial agents, especially from plants. The aims of this project were to examine the antimicrobial properties of essential oils distilled from Australian-grown Ocimum teniflorum (Tulsi), to quantify the volatile components present in flower spikes, leaves and the essential oil, and to investigate the compounds responsible for any activity. Broth micro-dilution was used to determine the minimum inhibitory concentration (MIC) of Tulsi essential oil against selected microbial pathogens. The oils, at concentrations of 4.5% and 2.25% completely inhibited the growth of Staphylococcus aureus (including MRSA) and Escherichia coli, while the same concentrations only partly inhibited the growth of Pseudomonas aeruginosa. Of 54 compounds identified in Tulsi leaves, flower spikes or essential oil, three are proposed to be responsible for this activity; camphor, eucalyptol and eugenol. Since S. aureus (including MRSA), P. aeruginosa and E. coli are major pathogens causing skin and soft tissue infections, Tulsi essential oil could be a valuable topical antimicrobial agent for management of skin infections caused by these organisms.http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.00681/fullAntimicrobial activityEssential oilTulsi (Ocimum tenuiflorum)Headspace–Solid Phase MicroextractionGas Chromatography–Mass Spectrometry. |
| spellingShingle | Hanaa Abbas M Yamani Hanaa Abbas M Yamani EDWIN CHI KYONG PANG Nitin eMantri Margaret Anne Deighton Antimicrobial activity of Tulsi (Ocimum tenuiflorum) essential oil and their major constituents against three species of bacteria Frontiers in Microbiology Antimicrobial activity Essential oil Tulsi (Ocimum tenuiflorum) Headspace–Solid Phase Microextraction Gas Chromatography–Mass Spectrometry. |
| title | Antimicrobial activity of Tulsi (Ocimum tenuiflorum) essential oil and their major constituents against three species of bacteria |
| title_full | Antimicrobial activity of Tulsi (Ocimum tenuiflorum) essential oil and their major constituents against three species of bacteria |
| title_fullStr | Antimicrobial activity of Tulsi (Ocimum tenuiflorum) essential oil and their major constituents against three species of bacteria |
| title_full_unstemmed | Antimicrobial activity of Tulsi (Ocimum tenuiflorum) essential oil and their major constituents against three species of bacteria |
| title_short | Antimicrobial activity of Tulsi (Ocimum tenuiflorum) essential oil and their major constituents against three species of bacteria |
| title_sort | antimicrobial activity of tulsi ocimum tenuiflorum essential oil and their major constituents against three species of bacteria |
| topic | Antimicrobial activity Essential oil Tulsi (Ocimum tenuiflorum) Headspace–Solid Phase Microextraction Gas Chromatography–Mass Spectrometry. |
| url | http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.00681/full |
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