Evaluation of Chimpanzee Adenovirus and MVA Expressing TRAP and CSP from <i>Plasmodium cynomolgi</i> to Prevent Malaria Relapse in Nonhuman Primates
<i>Plasmodium vivax</i> is the world’s most widely distributed human malaria parasite, with over 2.8 billion people at risk in Asia, the Americas, and Africa. The 80–90% new <i>P. vivax</i> malaria infections are due to relapses which suggest that a vaccine with high efficacy...
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| Language: | English |
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MDPI AG
2020-07-01
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| Series: | Vaccines |
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| Online Access: | https://www.mdpi.com/2076-393X/8/3/363 |
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| author | Young Chan Kim Barbara Dema Roberto Rodriguez-Garcia César López-Camacho Fabiana M. S. Leoratti Amar Lall Edmond J. Remarque Clemens H. M. Kocken Arturo Reyes-Sandoval |
| author_facet | Young Chan Kim Barbara Dema Roberto Rodriguez-Garcia César López-Camacho Fabiana M. S. Leoratti Amar Lall Edmond J. Remarque Clemens H. M. Kocken Arturo Reyes-Sandoval |
| author_sort | Young Chan Kim |
| collection | DOAJ |
| description | <i>Plasmodium vivax</i> is the world’s most widely distributed human malaria parasite, with over 2.8 billion people at risk in Asia, the Americas, and Africa. The 80–90% new <i>P. vivax</i> malaria infections are due to relapses which suggest that a vaccine with high efficacy against relapses by prevention of hypnozoite formation could lead to a significant reduction in the prevalence of <i>P. vivax</i> infections. Here, we describe the development of new recombinant ChAdOx1 and MVA vectors expressing <i>P. cynomolgi</i> Thrombospondin Related Adhesive Protein (PcTRAP) and the circumsporozoite protein (PcCSP). Both were shown to be immunogenic in mice prior to their assessment in rhesus macaques. We confirmed good vaccine-induced humoral and cellular responses after prime-boost vaccination in rhesus macaques prior to sporozoite challenge. Results indicate that there were no significant differences between mock-control and vaccinated animals after challenge, in terms of protective efficacy measured as the time taken to 1st patency, or as number of relapses. This suggests that under the conditions tested, the vaccination with PcTRAP and PcCSP using ChAdOx1 or MVA vaccine platforms do not protect against pre-erythrocytic malaria or relapses despite good immunogenicity induced by the viral-vectored vaccines. |
| first_indexed | 2024-03-10T18:39:12Z |
| format | Article |
| id | doaj.art-21a955844d644f07839aa23d15fb3aac |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-10T18:39:12Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-21a955844d644f07839aa23d15fb3aac2023-11-20T05:59:35ZengMDPI AGVaccines2076-393X2020-07-018336310.3390/vaccines8030363Evaluation of Chimpanzee Adenovirus and MVA Expressing TRAP and CSP from <i>Plasmodium cynomolgi</i> to Prevent Malaria Relapse in Nonhuman PrimatesYoung Chan Kim0Barbara Dema1Roberto Rodriguez-Garcia2César López-Camacho3Fabiana M. S. Leoratti4Amar Lall5Edmond J. Remarque6Clemens H. M. Kocken7Arturo Reyes-Sandoval8The Jenner Institute, University of Oxford, Old Road Campus Research Building, Roosevelt Drive, Oxford OX3 7DQ, UKThe Jenner Institute, University of Oxford, Old Road Campus Research Building, Roosevelt Drive, Oxford OX3 7DQ, UKDepartment of Parasitology, Biomedical Primate Research Centre (BPRC), 2288 GJ Rijswijk, The NetherlandsThe Jenner Institute, University of Oxford, Old Road Campus Research Building, Roosevelt Drive, Oxford OX3 7DQ, UKThe Jenner Institute, University of Oxford, Old Road Campus Research Building, Roosevelt Drive, Oxford OX3 7DQ, UKThe Jenner Institute, University of Oxford, Old Road Campus Research Building, Roosevelt Drive, Oxford OX3 7DQ, UKDepartment of Parasitology, Biomedical Primate Research Centre (BPRC), 2288 GJ Rijswijk, The NetherlandsDepartment of Parasitology, Biomedical Primate Research Centre (BPRC), 2288 GJ Rijswijk, The NetherlandsThe Jenner Institute, University of Oxford, Old Road Campus Research Building, Roosevelt Drive, Oxford OX3 7DQ, UK<i>Plasmodium vivax</i> is the world’s most widely distributed human malaria parasite, with over 2.8 billion people at risk in Asia, the Americas, and Africa. The 80–90% new <i>P. vivax</i> malaria infections are due to relapses which suggest that a vaccine with high efficacy against relapses by prevention of hypnozoite formation could lead to a significant reduction in the prevalence of <i>P. vivax</i> infections. Here, we describe the development of new recombinant ChAdOx1 and MVA vectors expressing <i>P. cynomolgi</i> Thrombospondin Related Adhesive Protein (PcTRAP) and the circumsporozoite protein (PcCSP). Both were shown to be immunogenic in mice prior to their assessment in rhesus macaques. We confirmed good vaccine-induced humoral and cellular responses after prime-boost vaccination in rhesus macaques prior to sporozoite challenge. Results indicate that there were no significant differences between mock-control and vaccinated animals after challenge, in terms of protective efficacy measured as the time taken to 1st patency, or as number of relapses. This suggests that under the conditions tested, the vaccination with PcTRAP and PcCSP using ChAdOx1 or MVA vaccine platforms do not protect against pre-erythrocytic malaria or relapses despite good immunogenicity induced by the viral-vectored vaccines.https://www.mdpi.com/2076-393X/8/3/363<i>P. vivax</i>Circumsporozoite proteinCSPthrombospondin related adhesive protein: TRAPadenovirusesMVA |
| spellingShingle | Young Chan Kim Barbara Dema Roberto Rodriguez-Garcia César López-Camacho Fabiana M. S. Leoratti Amar Lall Edmond J. Remarque Clemens H. M. Kocken Arturo Reyes-Sandoval Evaluation of Chimpanzee Adenovirus and MVA Expressing TRAP and CSP from <i>Plasmodium cynomolgi</i> to Prevent Malaria Relapse in Nonhuman Primates Vaccines <i>P. vivax</i> Circumsporozoite protein CSP thrombospondin related adhesive protein: TRAP adenoviruses MVA |
| title | Evaluation of Chimpanzee Adenovirus and MVA Expressing TRAP and CSP from <i>Plasmodium cynomolgi</i> to Prevent Malaria Relapse in Nonhuman Primates |
| title_full | Evaluation of Chimpanzee Adenovirus and MVA Expressing TRAP and CSP from <i>Plasmodium cynomolgi</i> to Prevent Malaria Relapse in Nonhuman Primates |
| title_fullStr | Evaluation of Chimpanzee Adenovirus and MVA Expressing TRAP and CSP from <i>Plasmodium cynomolgi</i> to Prevent Malaria Relapse in Nonhuman Primates |
| title_full_unstemmed | Evaluation of Chimpanzee Adenovirus and MVA Expressing TRAP and CSP from <i>Plasmodium cynomolgi</i> to Prevent Malaria Relapse in Nonhuman Primates |
| title_short | Evaluation of Chimpanzee Adenovirus and MVA Expressing TRAP and CSP from <i>Plasmodium cynomolgi</i> to Prevent Malaria Relapse in Nonhuman Primates |
| title_sort | evaluation of chimpanzee adenovirus and mva expressing trap and csp from i plasmodium cynomolgi i to prevent malaria relapse in nonhuman primates |
| topic | <i>P. vivax</i> Circumsporozoite protein CSP thrombospondin related adhesive protein: TRAP adenoviruses MVA |
| url | https://www.mdpi.com/2076-393X/8/3/363 |
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