Multiparametric analysis of anti-proliferative and apoptotic effects of gold nanoprisms on mouse and human primary and transformed cells, biodistribution and toxicity in vivo
Abstract Background The special physicochemical properties of gold nanoprisms make them very useful for biomedical applications including biosensing and cancer therapy. However, it is not clear how gold nanoprisms may affect cellular physiology including viability and other critical functions. We re...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2017-10-01
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| Series: | Particle and Fibre Toxicology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12989-017-0222-4 |
| _version_ | 1819051807067865088 |
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| author | Marta Pérez-Hernández María Moros Grazyna Stepien Pablo del Pino Sebastián Menao Marcelo de las Heras Maykel Arias Scott G. Mitchell Beatriz Pelaz Eva M. Gálvez Jesús M. de la Fuente Julián Pardo |
| author_facet | Marta Pérez-Hernández María Moros Grazyna Stepien Pablo del Pino Sebastián Menao Marcelo de las Heras Maykel Arias Scott G. Mitchell Beatriz Pelaz Eva M. Gálvez Jesús M. de la Fuente Julián Pardo |
| author_sort | Marta Pérez-Hernández |
| collection | DOAJ |
| description | Abstract Background The special physicochemical properties of gold nanoprisms make them very useful for biomedical applications including biosensing and cancer therapy. However, it is not clear how gold nanoprisms may affect cellular physiology including viability and other critical functions. We report a multiparametric investigation on the impact of gold-nanoprisms on mice and human, transformed and primary cells as well as tissue distribution and toxicity in vivo after parental injection. Methods Cellular uptake of the gold-nanoprisms (NPRs) and the most crucial parameters of cell fitness such as generation of reactive oxygen species (ROS), mitochondria membrane potential, cell morphology and apoptosis were systematically assayed in cells. Organ distribution and toxicity including inflammatory response were analysed in vivo in mice at 3 days or 4 months after parental administration. Results Internalized gold-nanoprisms have a significant impact in cell morphology, mitochondrial function and ROS production, which however do not affect the potential of cells to proliferate and form colonies. In vivo NPRs were only detected in spleen and liver at 3 days and 4 months after administration, which correlated with some changes in tissue architecture. However, the main serum biochemical markers of organ damage and inflammation (TNFα and IFNγ) remained unaltered even after 4 months. In addition, animals did not show any macroscopic sign of toxicity and remained healthy during all the study period. Conclusion Our data indicate that these gold-nanoprisms are neither cytotoxic nor cytostatic in transformed and primary cells, and suggest that extensive parameters should be analysed in different cell types to draw useful conclusions on nanomaterials safety. Moreover, although there is a tendency for the NPRs to accumulate in liver and spleen, there is no observable negative impact on animal health. |
| first_indexed | 2024-12-21T12:09:48Z |
| format | Article |
| id | doaj.art-21a9fc49f2dc42339a6c41f094e8adbc |
| institution | Directory Open Access Journal |
| issn | 1743-8977 |
| language | English |
| last_indexed | 2024-12-21T12:09:48Z |
| publishDate | 2017-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Particle and Fibre Toxicology |
| spelling | doaj.art-21a9fc49f2dc42339a6c41f094e8adbc2022-12-21T19:04:36ZengBMCParticle and Fibre Toxicology1743-89772017-10-0114112010.1186/s12989-017-0222-4Multiparametric analysis of anti-proliferative and apoptotic effects of gold nanoprisms on mouse and human primary and transformed cells, biodistribution and toxicity in vivoMarta Pérez-Hernández0María Moros1Grazyna Stepien2Pablo del Pino3Sebastián Menao4Marcelo de las Heras5Maykel Arias6Scott G. Mitchell7Beatriz Pelaz8Eva M. Gálvez9Jesús M. de la Fuente10Julián Pardo11Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Ciencias, Universidad de ZaragozaInstituto Universitario de Nanociencia de Aragón (INA), Universidad de ZaragozaFundación Instituto Universitario de Nanociencia de Aragón (FINA), Universidad de ZaragozaInstituto Universitario de Nanociencia de Aragón (INA), Universidad de ZaragozaDepartamento de Bioquímica clínica. H.C.U. Lozano BlesaDepartamento de Patología Animal, Facultad de Veterinaria, Universidad de ZaragozaInstituto de Investigación Sanitaria de Aragón (IIS Aragón), Centro de Investigación Biomédica de Aragón (CIBA), Universidad de ZaragozaInstituto de Ciencia de Materiales de Aragón, CSIC-Universidad de ZaragozaInstituto Universitario de Nanociencia de Aragón (INA), Universidad de ZaragozaInstituto de Carboquímica ICB-CSICCIBER in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN)Instituto de Investigación Sanitaria de Aragón (IIS Aragón), Centro de Investigación Biomédica de Aragón (CIBA), Universidad de ZaragozaAbstract Background The special physicochemical properties of gold nanoprisms make them very useful for biomedical applications including biosensing and cancer therapy. However, it is not clear how gold nanoprisms may affect cellular physiology including viability and other critical functions. We report a multiparametric investigation on the impact of gold-nanoprisms on mice and human, transformed and primary cells as well as tissue distribution and toxicity in vivo after parental injection. Methods Cellular uptake of the gold-nanoprisms (NPRs) and the most crucial parameters of cell fitness such as generation of reactive oxygen species (ROS), mitochondria membrane potential, cell morphology and apoptosis were systematically assayed in cells. Organ distribution and toxicity including inflammatory response were analysed in vivo in mice at 3 days or 4 months after parental administration. Results Internalized gold-nanoprisms have a significant impact in cell morphology, mitochondrial function and ROS production, which however do not affect the potential of cells to proliferate and form colonies. In vivo NPRs were only detected in spleen and liver at 3 days and 4 months after administration, which correlated with some changes in tissue architecture. However, the main serum biochemical markers of organ damage and inflammation (TNFα and IFNγ) remained unaltered even after 4 months. In addition, animals did not show any macroscopic sign of toxicity and remained healthy during all the study period. Conclusion Our data indicate that these gold-nanoprisms are neither cytotoxic nor cytostatic in transformed and primary cells, and suggest that extensive parameters should be analysed in different cell types to draw useful conclusions on nanomaterials safety. Moreover, although there is a tendency for the NPRs to accumulate in liver and spleen, there is no observable negative impact on animal health.http://link.springer.com/article/10.1186/s12989-017-0222-4Gold nanoprismsNanotoxicologyROS generationMitochondrial membrane-potentialBiodistributionIn vivo |
| spellingShingle | Marta Pérez-Hernández María Moros Grazyna Stepien Pablo del Pino Sebastián Menao Marcelo de las Heras Maykel Arias Scott G. Mitchell Beatriz Pelaz Eva M. Gálvez Jesús M. de la Fuente Julián Pardo Multiparametric analysis of anti-proliferative and apoptotic effects of gold nanoprisms on mouse and human primary and transformed cells, biodistribution and toxicity in vivo Particle and Fibre Toxicology Gold nanoprisms Nanotoxicology ROS generation Mitochondrial membrane-potential Biodistribution In vivo |
| title | Multiparametric analysis of anti-proliferative and apoptotic effects of gold nanoprisms on mouse and human primary and transformed cells, biodistribution and toxicity in vivo |
| title_full | Multiparametric analysis of anti-proliferative and apoptotic effects of gold nanoprisms on mouse and human primary and transformed cells, biodistribution and toxicity in vivo |
| title_fullStr | Multiparametric analysis of anti-proliferative and apoptotic effects of gold nanoprisms on mouse and human primary and transformed cells, biodistribution and toxicity in vivo |
| title_full_unstemmed | Multiparametric analysis of anti-proliferative and apoptotic effects of gold nanoprisms on mouse and human primary and transformed cells, biodistribution and toxicity in vivo |
| title_short | Multiparametric analysis of anti-proliferative and apoptotic effects of gold nanoprisms on mouse and human primary and transformed cells, biodistribution and toxicity in vivo |
| title_sort | multiparametric analysis of anti proliferative and apoptotic effects of gold nanoprisms on mouse and human primary and transformed cells biodistribution and toxicity in vivo |
| topic | Gold nanoprisms Nanotoxicology ROS generation Mitochondrial membrane-potential Biodistribution In vivo |
| url | http://link.springer.com/article/10.1186/s12989-017-0222-4 |
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