Tissue-Engineered Vascular Graft with Co-Culture of Smooth Muscle Cells and Human Endothelial Vein Cells on an Electrospun Poly(lactic-co-glycolic acid) Microtube Array Membrane
Coronary artery disease is one of the major diseases that plagues today’s modern society. Conventional treatments utilize synthetic vascular grafts such as Dacron<sup>®</sup> and Teflon<sup>®</sup> in bypass graft surgery. Despite the wide adaptation, these synthetic grafts a...
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MDPI AG
2021-09-01
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| Series: | Membranes |
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| Online Access: | https://www.mdpi.com/2077-0375/11/10/732 |
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| author | Chee Ho Chew Bo-Long Sheu Amanda Chen Wan-Ting Huang Tsai-Mu Cheng Chun-Ming Shih Austin Chang Chien-Chung Chen |
| author_facet | Chee Ho Chew Bo-Long Sheu Amanda Chen Wan-Ting Huang Tsai-Mu Cheng Chun-Ming Shih Austin Chang Chien-Chung Chen |
| author_sort | Chee Ho Chew |
| collection | DOAJ |
| description | Coronary artery disease is one of the major diseases that plagues today’s modern society. Conventional treatments utilize synthetic vascular grafts such as Dacron<sup>®</sup> and Teflon<sup>®</sup> in bypass graft surgery. Despite the wide adaptation, these synthetic grafts are often plagued with weaknesses such as low hemocompatibility, thrombosis, intimal hyperplasia, and risks of graft infection. More importantly, these synthetic grafts are not available at diameters of less than 6 mm. In view of these challenges, we strived to develop and adapt the electrospun Poly Lactic-co-Glycolic Acid (PLGA) Microtube Array Membrane (MTAM) vascular graft for applications smaller than 6 mm in diameter. Homogenously porous PLGA MTAMs were successfully electrospun at 5.5–8.5 kV under ambient conditions. Mechanically, the PLGA MTAMs registered a maximum tensile strength of 5.57 ± 0.85 MPa and Young’s modulus value of 1.134 ± 0.01 MPa; while MTT assay revealed that seven-day Smooth Muscle Cells (SMCs) and Human Umbilical Vein Endothelial Cells (HUVECs) registered a 6 times and 2.4 times higher cell viability when cultured in a co-culture setting in medium containing α-1 haptaglobulin. When rolled into a vascular graft, the PLGA MTAMs registered an overall degradation of 82% after 60 days of cell co-culture. After eight weeks of culturing, immunohistochemistry staining revealed the formation of a monolayer of HUVECs with tight junctions on the surface of the PLGA MTAM, and as for the SMCs housed within the lumens of the PLGA MTAMs, a monolayer with high degree of orientation was observed. The PLGA MTAM registered a burst pressure of 1092.2 ± 175.3 mmHg, which was sufficient for applications such as small diameter blood vessels. Potentially, the PLGA MTAM could be used as a suitable substrate for vascular engineering. |
| first_indexed | 2024-03-10T06:23:43Z |
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| id | doaj.art-21ad3f4f5b0344dd9f989e833f8ee05d |
| institution | Directory Open Access Journal |
| issn | 2077-0375 |
| language | English |
| last_indexed | 2024-03-10T06:23:43Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Membranes |
| spelling | doaj.art-21ad3f4f5b0344dd9f989e833f8ee05d2023-11-22T19:05:45ZengMDPI AGMembranes2077-03752021-09-01111073210.3390/membranes11100732Tissue-Engineered Vascular Graft with Co-Culture of Smooth Muscle Cells and Human Endothelial Vein Cells on an Electrospun Poly(lactic-co-glycolic acid) Microtube Array MembraneChee Ho Chew0Bo-Long Sheu1Amanda Chen2Wan-Ting Huang3Tsai-Mu Cheng4Chun-Ming Shih5Austin Chang6Chien-Chung Chen7Graduate Institute of Biomedical Materials & Tissue Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei 11052, TaiwanGraduate Institute of Biomedical Materials & Tissue Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei 11052, TaiwanDepartment of Biochemistry, University of Washington, Seattle, WA 98195, USAGraduate Institute of Biomedical Materials & Tissue Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei 11052, TaiwanThe Ph.D. Program for Translational Medicine, Taipei Medical University, Taipei 11052, TaiwanTaipei Heart Institute (THI), Taipei Medical University, Taipei 11052, TaiwanCore Facility Center, Taipei Medical University, Taipei 11052, TaiwanGraduate Institute of Biomedical Materials & Tissue Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei 11052, TaiwanCoronary artery disease is one of the major diseases that plagues today’s modern society. Conventional treatments utilize synthetic vascular grafts such as Dacron<sup>®</sup> and Teflon<sup>®</sup> in bypass graft surgery. Despite the wide adaptation, these synthetic grafts are often plagued with weaknesses such as low hemocompatibility, thrombosis, intimal hyperplasia, and risks of graft infection. More importantly, these synthetic grafts are not available at diameters of less than 6 mm. In view of these challenges, we strived to develop and adapt the electrospun Poly Lactic-co-Glycolic Acid (PLGA) Microtube Array Membrane (MTAM) vascular graft for applications smaller than 6 mm in diameter. Homogenously porous PLGA MTAMs were successfully electrospun at 5.5–8.5 kV under ambient conditions. Mechanically, the PLGA MTAMs registered a maximum tensile strength of 5.57 ± 0.85 MPa and Young’s modulus value of 1.134 ± 0.01 MPa; while MTT assay revealed that seven-day Smooth Muscle Cells (SMCs) and Human Umbilical Vein Endothelial Cells (HUVECs) registered a 6 times and 2.4 times higher cell viability when cultured in a co-culture setting in medium containing α-1 haptaglobulin. When rolled into a vascular graft, the PLGA MTAMs registered an overall degradation of 82% after 60 days of cell co-culture. After eight weeks of culturing, immunohistochemistry staining revealed the formation of a monolayer of HUVECs with tight junctions on the surface of the PLGA MTAM, and as for the SMCs housed within the lumens of the PLGA MTAMs, a monolayer with high degree of orientation was observed. The PLGA MTAM registered a burst pressure of 1092.2 ± 175.3 mmHg, which was sufficient for applications such as small diameter blood vessels. Potentially, the PLGA MTAM could be used as a suitable substrate for vascular engineering.https://www.mdpi.com/2077-0375/11/10/732Poly(lactic-co-glycolic acid) (PLGA)Microtube Array Membrane (MTAM)electrospinningTissue Engineered Vascular Grafts (TEVG)Smooth Muscle Cells (SMCs)Human Endothelial Vein Cells (HUVECs) |
| spellingShingle | Chee Ho Chew Bo-Long Sheu Amanda Chen Wan-Ting Huang Tsai-Mu Cheng Chun-Ming Shih Austin Chang Chien-Chung Chen Tissue-Engineered Vascular Graft with Co-Culture of Smooth Muscle Cells and Human Endothelial Vein Cells on an Electrospun Poly(lactic-co-glycolic acid) Microtube Array Membrane Membranes Poly(lactic-co-glycolic acid) (PLGA) Microtube Array Membrane (MTAM) electrospinning Tissue Engineered Vascular Grafts (TEVG) Smooth Muscle Cells (SMCs) Human Endothelial Vein Cells (HUVECs) |
| title | Tissue-Engineered Vascular Graft with Co-Culture of Smooth Muscle Cells and Human Endothelial Vein Cells on an Electrospun Poly(lactic-co-glycolic acid) Microtube Array Membrane |
| title_full | Tissue-Engineered Vascular Graft with Co-Culture of Smooth Muscle Cells and Human Endothelial Vein Cells on an Electrospun Poly(lactic-co-glycolic acid) Microtube Array Membrane |
| title_fullStr | Tissue-Engineered Vascular Graft with Co-Culture of Smooth Muscle Cells and Human Endothelial Vein Cells on an Electrospun Poly(lactic-co-glycolic acid) Microtube Array Membrane |
| title_full_unstemmed | Tissue-Engineered Vascular Graft with Co-Culture of Smooth Muscle Cells and Human Endothelial Vein Cells on an Electrospun Poly(lactic-co-glycolic acid) Microtube Array Membrane |
| title_short | Tissue-Engineered Vascular Graft with Co-Culture of Smooth Muscle Cells and Human Endothelial Vein Cells on an Electrospun Poly(lactic-co-glycolic acid) Microtube Array Membrane |
| title_sort | tissue engineered vascular graft with co culture of smooth muscle cells and human endothelial vein cells on an electrospun poly lactic co glycolic acid microtube array membrane |
| topic | Poly(lactic-co-glycolic acid) (PLGA) Microtube Array Membrane (MTAM) electrospinning Tissue Engineered Vascular Grafts (TEVG) Smooth Muscle Cells (SMCs) Human Endothelial Vein Cells (HUVECs) |
| url | https://www.mdpi.com/2077-0375/11/10/732 |
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