Agrobacterium-mediated transformation of the wild orchid Cattleya maxima Lindl
Protocorms are unique anatomical structures; they are akin to rhizoids and are formed by young orchid seedlings under physiological conditions. Explanted orchid tissues produce similar structures called protocorm-like bodies (PLBs) when exposed to appropriate in vitro growing conditions. Both th...
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| Format: | Article |
| Language: | English |
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Pontificia Universidad Javeriana
2018-03-01
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| Series: | Universitas Scientiarum |
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| Online Access: | http://revistas.javeriana.edu.co/index.php/scientarium/article/view/19526/16941 |
| _version_ | 1811298955860901888 |
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| author | Augusta Yadira Cueva-Agila Rino Cella |
| author_facet | Augusta Yadira Cueva-Agila Rino Cella |
| author_sort | Augusta Yadira Cueva-Agila |
| collection | DOAJ |
| description | Protocorms are unique anatomical structures; they are akin to rhizoids
and are formed by young orchid seedlings under physiological conditions.
Explanted orchid tissues produce similar structures called protocorm-like
bodies (PLBs) when exposed to appropriate in vitro growing conditions.
Both the propagative nature of PLBs and the easiness by which they can
be generated, make these structures an attractive alternative to seed-mediated
production for growing large numbers of plants. To increase somatic
embryogenesis and optimize the procedure, PLBs of Cattleya maxima were
transformed using the Agrobacterium tumefaciens method. The T-DNA carried
a Hygromycin-resistance gene, a visible marker (GFP5-GUSA) and a rice
gene encoding the Somatic Embryogenesis Receptor Kinase, deemed to
be important for somatic embryogenesis. Treated PLBs generated somatic
embryos developing Hygromycin-resistant plantlets. The insertion of T-DNA
was confirmed by PCR, and GFP expression was observed using a fluorescent
stereomicroscope. Transformed Cattleya maxima PLBs were more efficient in
forming somatic embryos (60 - 80 %) than untransformed controls (45 - 57 %),
and this contrast was maximized in hormone-free, Murashige and Skoog
(MS) medium (80 % of the transformed plants compared to 57 % of the
untransformed ones). This finding supports the notion that SERK plays an
important role in Orchid embryogenesis |
| first_indexed | 2024-04-13T06:27:56Z |
| format | Article |
| id | doaj.art-21ad55778fdd4fec8b2354c4815db53f |
| institution | Directory Open Access Journal |
| issn | 0122-7483 2027-1352 |
| language | English |
| last_indexed | 2024-04-13T06:27:56Z |
| publishDate | 2018-03-01 |
| publisher | Pontificia Universidad Javeriana |
| record_format | Article |
| series | Universitas Scientiarum |
| spelling | doaj.art-21ad55778fdd4fec8b2354c4815db53f2022-12-22T02:58:17ZengPontificia Universidad JaverianaUniversitas Scientiarum0122-74832027-13522018-03-012318910710.11144/Javeriana.SC23-1.amtoAgrobacterium-mediated transformation of the wild orchid Cattleya maxima LindlAugusta Yadira Cueva-Agila0Rino Cella1 Departamento de Ciencias Biológicas, Universidad Técnica Particular de Loja, Loja, Ecuador.Department of Biology and Biotechnology. University of Pavia, Via Ferrata 9, 27100, Pavia, ItalyProtocorms are unique anatomical structures; they are akin to rhizoids and are formed by young orchid seedlings under physiological conditions. Explanted orchid tissues produce similar structures called protocorm-like bodies (PLBs) when exposed to appropriate in vitro growing conditions. Both the propagative nature of PLBs and the easiness by which they can be generated, make these structures an attractive alternative to seed-mediated production for growing large numbers of plants. To increase somatic embryogenesis and optimize the procedure, PLBs of Cattleya maxima were transformed using the Agrobacterium tumefaciens method. The T-DNA carried a Hygromycin-resistance gene, a visible marker (GFP5-GUSA) and a rice gene encoding the Somatic Embryogenesis Receptor Kinase, deemed to be important for somatic embryogenesis. Treated PLBs generated somatic embryos developing Hygromycin-resistant plantlets. The insertion of T-DNA was confirmed by PCR, and GFP expression was observed using a fluorescent stereomicroscope. Transformed Cattleya maxima PLBs were more efficient in forming somatic embryos (60 - 80 %) than untransformed controls (45 - 57 %), and this contrast was maximized in hormone-free, Murashige and Skoog (MS) medium (80 % of the transformed plants compared to 57 % of the untransformed ones). This finding supports the notion that SERK plays an important role in Orchid embryogenesishttp://revistas.javeriana.edu.co/index.php/scientarium/article/view/19526/16941Protocorm-Like-BodiesTransformationOrchidspCAMBIASERKAgrobacterium tumefaciens |
| spellingShingle | Augusta Yadira Cueva-Agila Rino Cella Agrobacterium-mediated transformation of the wild orchid Cattleya maxima Lindl Universitas Scientiarum Protocorm-Like-Bodies Transformation Orchids pCAMBIA SERK Agrobacterium tumefaciens |
| title | Agrobacterium-mediated transformation of the wild orchid Cattleya maxima Lindl |
| title_full | Agrobacterium-mediated transformation of the wild orchid Cattleya maxima Lindl |
| title_fullStr | Agrobacterium-mediated transformation of the wild orchid Cattleya maxima Lindl |
| title_full_unstemmed | Agrobacterium-mediated transformation of the wild orchid Cattleya maxima Lindl |
| title_short | Agrobacterium-mediated transformation of the wild orchid Cattleya maxima Lindl |
| title_sort | agrobacterium mediated transformation of the wild orchid cattleya maxima lindl |
| topic | Protocorm-Like-Bodies Transformation Orchids pCAMBIA SERK Agrobacterium tumefaciens |
| url | http://revistas.javeriana.edu.co/index.php/scientarium/article/view/19526/16941 |
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