Effect of Aqueous Enzymatic Extraction of Deer Oil on Its Components and Its Protective Effect on Gastric Mucosa Injury
In this study, deer suet fat was used as a raw material to study the effects of aqueous enzymatic extraction of deer oil on its components, followed by studies into the potential protective activity, and related molecular mechanisms of deer oil on ethanol-induced acute gastric mucosal injury in rats...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-11-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2021.769463/full |
| _version_ | 1819041285595463680 |
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| author | Yun-Shi Xia Yun-Shi Xia Yin-shi Sun Yin-shi Sun Chang Liu Zhi-Man Li Duo-Duo Ren Duo-Duo Ren Rui Mu Rui Mu Yan-Ting Zhang Pan-Pan Bo Pan-Pan Bo Li-juan Zhao Zi Wang |
| author_facet | Yun-Shi Xia Yun-Shi Xia Yin-shi Sun Yin-shi Sun Chang Liu Zhi-Man Li Duo-Duo Ren Duo-Duo Ren Rui Mu Rui Mu Yan-Ting Zhang Pan-Pan Bo Pan-Pan Bo Li-juan Zhao Zi Wang |
| author_sort | Yun-Shi Xia |
| collection | DOAJ |
| description | In this study, deer suet fat was used as a raw material to study the effects of aqueous enzymatic extraction of deer oil on its components, followed by studies into the potential protective activity, and related molecular mechanisms of deer oil on ethanol-induced acute gastric mucosal injury in rats. The results show that aqueous enzymatic extraction of deer oil not only has a high extraction yield and has a small effect on the content of active ingredients. Deer oil can reduce total stomach injury. Without affecting the blood lipid level, it can reduce the oxidative stress, which is manifested by reducing the content of myeloperoxidase (MPO) and enhancing the activity level of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px). It also enhances the expression of defense factors prostaglandin (E2), epidermal growth factor (EGF), and somatostatin (SS), it inhibits apoptosis evidenced by the enhanced of Bcl-2 and decreased expression of cleavage of caspase-3 and Bax. At the same time, it reduces inflammation, which is manifested by reducing the expression of IL-1β, interleukin 6 (IL-6), and tumor necrosis factor alpha (TNF-α) gastric tissue pro-inflammatory cytokines, and enhancing the expression of anti-inflammatory factors IL-4 and IL-10, and inhibiting the mitogen-activated protein kinase/nuclear factor kappa B (MAPK/NF-κB) signaling pathway in gastric tissue. |
| first_indexed | 2024-12-21T09:22:34Z |
| format | Article |
| id | doaj.art-21b05b8704b94ea5a0a223d87da0062d |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-12-21T09:22:34Z |
| publishDate | 2021-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj.art-21b05b8704b94ea5a0a223d87da0062d2022-12-21T19:08:58ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2021-11-01810.3389/fnut.2021.769463769463Effect of Aqueous Enzymatic Extraction of Deer Oil on Its Components and Its Protective Effect on Gastric Mucosa InjuryYun-Shi Xia0Yun-Shi Xia1Yin-shi Sun2Yin-shi Sun3Chang Liu4Zhi-Man Li5Duo-Duo Ren6Duo-Duo Ren7Rui Mu8Rui Mu9Yan-Ting Zhang10Pan-Pan Bo11Pan-Pan Bo12Li-juan Zhao13Zi Wang14College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun, ChinaInstitute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences, Changchun, ChinaCollege of Chinese Medicinal Materials, Jilin Agricultural University, Changchun, ChinaInstitute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences, Changchun, ChinaCollege of Food Science and Engineering, Changchun University, Changchun, ChinaInstitute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences, Changchun, ChinaCollege of Chinese Medicinal Materials, Jilin Agricultural University, Changchun, ChinaInstitute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences, Changchun, ChinaCollege of Chinese Medicinal Materials, Jilin Agricultural University, Changchun, ChinaInstitute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences, Changchun, ChinaInstitute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences, Changchun, ChinaCollege of Chinese Medicinal Materials, Jilin Agricultural University, Changchun, ChinaInstitute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences, Changchun, ChinaInstitute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences, Changchun, ChinaCollege of Chinese Medicinal Materials, Jilin Agricultural University, Changchun, ChinaIn this study, deer suet fat was used as a raw material to study the effects of aqueous enzymatic extraction of deer oil on its components, followed by studies into the potential protective activity, and related molecular mechanisms of deer oil on ethanol-induced acute gastric mucosal injury in rats. The results show that aqueous enzymatic extraction of deer oil not only has a high extraction yield and has a small effect on the content of active ingredients. Deer oil can reduce total stomach injury. Without affecting the blood lipid level, it can reduce the oxidative stress, which is manifested by reducing the content of myeloperoxidase (MPO) and enhancing the activity level of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px). It also enhances the expression of defense factors prostaglandin (E2), epidermal growth factor (EGF), and somatostatin (SS), it inhibits apoptosis evidenced by the enhanced of Bcl-2 and decreased expression of cleavage of caspase-3 and Bax. At the same time, it reduces inflammation, which is manifested by reducing the expression of IL-1β, interleukin 6 (IL-6), and tumor necrosis factor alpha (TNF-α) gastric tissue pro-inflammatory cytokines, and enhancing the expression of anti-inflammatory factors IL-4 and IL-10, and inhibiting the mitogen-activated protein kinase/nuclear factor kappa B (MAPK/NF-κB) signaling pathway in gastric tissue.https://www.frontiersin.org/articles/10.3389/fnut.2021.769463/fulldeer oilaqueous enzymatic extractionethanolgastric mucosal injuryMAPK pathwayNF-κB pathway |
| spellingShingle | Yun-Shi Xia Yun-Shi Xia Yin-shi Sun Yin-shi Sun Chang Liu Zhi-Man Li Duo-Duo Ren Duo-Duo Ren Rui Mu Rui Mu Yan-Ting Zhang Pan-Pan Bo Pan-Pan Bo Li-juan Zhao Zi Wang Effect of Aqueous Enzymatic Extraction of Deer Oil on Its Components and Its Protective Effect on Gastric Mucosa Injury Frontiers in Nutrition deer oil aqueous enzymatic extraction ethanol gastric mucosal injury MAPK pathway NF-κB pathway |
| title | Effect of Aqueous Enzymatic Extraction of Deer Oil on Its Components and Its Protective Effect on Gastric Mucosa Injury |
| title_full | Effect of Aqueous Enzymatic Extraction of Deer Oil on Its Components and Its Protective Effect on Gastric Mucosa Injury |
| title_fullStr | Effect of Aqueous Enzymatic Extraction of Deer Oil on Its Components and Its Protective Effect on Gastric Mucosa Injury |
| title_full_unstemmed | Effect of Aqueous Enzymatic Extraction of Deer Oil on Its Components and Its Protective Effect on Gastric Mucosa Injury |
| title_short | Effect of Aqueous Enzymatic Extraction of Deer Oil on Its Components and Its Protective Effect on Gastric Mucosa Injury |
| title_sort | effect of aqueous enzymatic extraction of deer oil on its components and its protective effect on gastric mucosa injury |
| topic | deer oil aqueous enzymatic extraction ethanol gastric mucosal injury MAPK pathway NF-κB pathway |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2021.769463/full |
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