Antigen incorporation on Cryptosporidium parvum oocyst walls
Cryptosporidium parvum oocysts are the infective stages responsible for transmission and survival of the organism in the environment. In the present work we show that the oocyst wall, far from being a static structure, is able to incorporate antigens by a mechanism involving vesicle fusion with the...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2001-01-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762001000200016 |
| _version_ | 1797718167762501632 |
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| author | Entrala Emilio Sbihi Younes Sánchez-Moreno Manuel Mascaró Carmen |
| author_facet | Entrala Emilio Sbihi Younes Sánchez-Moreno Manuel Mascaró Carmen |
| author_sort | Entrala Emilio |
| collection | DOAJ |
| description | Cryptosporidium parvum oocysts are the infective stages responsible for transmission and survival of the organism in the environment. In the present work we show that the oocyst wall, far from being a static structure, is able to incorporate antigens by a mechanism involving vesicle fusion with the wall, and the incorporation of the antigen to the outer oocyst wall. Using immunoelectron microscopy we show that the antigen recognized by a monoclonal antibody used for diagnosis of cryptosporidiosis (Merifluor®, Meridian Diagnostic Inc.) could be found associated with vesicles in the space between the sporozoites and the oocysts wall, and incorporated to the outer oocyst wall by an unknown mechanism. |
| first_indexed | 2024-03-12T08:46:33Z |
| format | Article |
| id | doaj.art-21b19798d8064123b24c55194e174651 |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T08:46:33Z |
| publishDate | 2001-01-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-21b19798d8064123b24c55194e1746512023-09-02T16:25:26ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80602001-01-01962233235Antigen incorporation on Cryptosporidium parvum oocyst wallsEntrala EmilioSbihi YounesSánchez-Moreno ManuelMascaró CarmenCryptosporidium parvum oocysts are the infective stages responsible for transmission and survival of the organism in the environment. In the present work we show that the oocyst wall, far from being a static structure, is able to incorporate antigens by a mechanism involving vesicle fusion with the wall, and the incorporation of the antigen to the outer oocyst wall. Using immunoelectron microscopy we show that the antigen recognized by a monoclonal antibody used for diagnosis of cryptosporidiosis (Merifluor®, Meridian Diagnostic Inc.) could be found associated with vesicles in the space between the sporozoites and the oocysts wall, and incorporated to the outer oocyst wall by an unknown mechanism.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762001000200016Cryptosporidiumoocyst wallantigen |
| spellingShingle | Entrala Emilio Sbihi Younes Sánchez-Moreno Manuel Mascaró Carmen Antigen incorporation on Cryptosporidium parvum oocyst walls Memorias do Instituto Oswaldo Cruz Cryptosporidium oocyst wall antigen |
| title | Antigen incorporation on Cryptosporidium parvum oocyst walls |
| title_full | Antigen incorporation on Cryptosporidium parvum oocyst walls |
| title_fullStr | Antigen incorporation on Cryptosporidium parvum oocyst walls |
| title_full_unstemmed | Antigen incorporation on Cryptosporidium parvum oocyst walls |
| title_short | Antigen incorporation on Cryptosporidium parvum oocyst walls |
| title_sort | antigen incorporation on cryptosporidium parvum oocyst walls |
| topic | Cryptosporidium oocyst wall antigen |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762001000200016 |
| work_keys_str_mv | AT entralaemilio antigenincorporationoncryptosporidiumparvumoocystwalls AT sbihiyounes antigenincorporationoncryptosporidiumparvumoocystwalls AT sanchezmorenomanuel antigenincorporationoncryptosporidiumparvumoocystwalls AT mascarocarmen antigenincorporationoncryptosporidiumparvumoocystwalls |